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AB115772

Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8]

5

(1 Review)

|

(2 Publications)

Mouse Monoclonal Apolipoprotein A V/APOA5 antibody. Suitable for WB, sELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APOA5.

View Alternative Names

RAP3, UNQ411/PRO773, APOA5, Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3

3 Images
Sandwich ELISA - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (AB115772)
  • sELISA

Unknown

Sandwich ELISA - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (AB115772)

Sandwich ELISA using antibody pair to detect human Apolipoprotein A V/APOA5 protein.

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (AB115772)
  • WB

Unknown

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (AB115772)

All lanes:

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (ab115772) at 1/500 dilution

All lanes:

Human serum

Predicted band size: 41 kDa

false

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (AB115772)
  • WB

Unknown

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (AB115772)

All lanes:

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [2G1H11, 1F1E8] (ab115772) at 1/500 dilution

All lanes:

Human recombinant Apolipoprotein A V/APOA5 protein

Predicted band size: 41 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G1H11, 1F1E8

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human APOA5. The exact immunogen used to generate this antibody is proprietary information.

Q6Q788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1/10000", "sELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
Biological function summary

APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.

Pathways

APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.

APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).
See full target information APOA5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 51: PubMed37026514

2023

Overexpression of salusin‑α upregulates AdipoR2 and activates the PPARα/ApoA5/SREBP‑1c pathway to inhibit lipid synthesis in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Zhang,Chao Yang,Songjiao Wang,Aohong Xu,Qian Zhang,Xiuqun Duan,Guofu Gong,Yuxue Wang

Computational and structural biotechnology journal 20:4717-4732 PubMed36147669

2022

Integrating knowledge of protein sequence with protein function for the prediction and validation of new MALT1 substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Peter A Bell,Sophia Scheuermann,Florian Renner,Christina L Pan,Henry Y Lu,Stuart E Turvey,Frédéric Bornancin,Catherine H Régnier,Christopher M Overall
View all publications
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Product promise

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