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AB239579

Anti-Apolipoprotein A V/APOA5 antibody [C6]

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(2 Publications)

Mouse Monoclonal Apolipoprotein A V/APOA5 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Apoa5 aa 1-250.

View Alternative Names

Rap3, Apoa5, Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A V/APOA5 antibody [C6] (AB239579)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A V/APOA5 antibody [C6] (AB239579)

Paraffin-embedded mouse liver tissue stained for Apolipoprotein A V/APOA5 using ab239579 at 30 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [C6] (AB239579)
  • WB

Supplier Data

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [C6] (AB239579)

All lanes:

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [C6] (ab239579) at 1 µg/mL

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 41 kDa

false

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [C6] (AB239579)
  • WB

Supplier Data

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [C6] (AB239579)

All lanes:

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [C6] (ab239579) at 5 µg/mL

All lanes:

Recombinant Mouse Apolipoprotein A V/APOA5 protein

Predicted band size: 41 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C6

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Apoa5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q8C7G5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
Biological function summary

APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.

Pathways

APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.

APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages (By similarity). Binds heparin (By similarity).
See full target information Apoa5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal science 100: PubMed35235945

2022

Increasing serotonin concentrations alter calcium metabolism in periparturient dairy goats.

Applications

Unspecified application

Species

Unspecified reactive species

ZhiFei Zhang,Wei Du,WenYi Liu,Braden T Wong,HuiLing Zheng

Frontiers in physiology 12:783341 PubMed35035359

2021

Exercise Alleviates the Apolipoprotein A5-Toll-Like Receptor 4 Axis Impairment in Mice With High-Fat Diet-Induced Non-alcoholic Steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yu,Lina Yu,Nuo Cheng,Xiaoguang Liu,Chunlu Fang,Shujing Liu,Lin Zhu
View all publications
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