Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68]

• IP

Supplier Data

Immunoprecipitation - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] (AB313368)

Apolipoprotein A V/APOA5 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab313368 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313368 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : ab313368 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313368 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] (ab313368) at 1/1000 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

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• WB

Supplier Data

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] (AB313368)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : skeletal muscle, lung (PMID : 11577099), HeLa, THP-1 (PMID : 21489765). The blot of lane 5 was developed using a high sensitivity ECL substrate. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : Lanes 1-4 : 92 seconds; Lane 5 : 180 seconds; Lanes 6-8 : 92 seconds.

All lanes:

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] (ab313368) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human serum at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Lane 5:

Human liver tissue lysate at 20 µg

Lane 6:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 8:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 6 - 8:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 41 kDa

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