AB20737

Anti-Apolipoprotein B antibody

Name: Anti-Apolipoprotein B antibody (ab20737) | Abcam
Brand: Abcam Limited
SKU: AB20737
Price: 430 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(99 Publications)

Anti-Apolipoprotein B antibody (ab20737) is a rabbit polyclonal antibody detecting Apolipoprotein B in Western Blot. Suitable for Human, Mouse.

- Over 80 publications
- Trusted since 2005

View Alternative Names

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Mouse Apob.

E9Q414

Specificity

Cross-reacts with Apo B48 and B100.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Apolipoprotein B antibody (ab20737) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.

What is the molecular weight of Apolipoprotein B?
Anti-Apolipoprotein B (ab20737) specifically detects a band for Apolipoprotein B (UniProt: P04114) at a molecular weight of 515kDa.

Trusted by the scientific community
Anti-Apolipoprotein B (ab20737) was first used in a scientific publication in 2005 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-Apolipoprotein B (ab20737) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Purification notes

Heat inactivated.Product is 0.22um filtered.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

See full target information APOB

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Publications (99)

Recent publications for all applications. Explore the full list and refine your search

Lipids in health and disease 24:239 PubMed40660209

2025

Lipid infiltration promotes trans-differentiation of vascular smooth muscle cells into macrophage-like cells in early lesions of human coronary atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junhai Hao,Jiang Liu,Jiahui Zhou,Yuanfeng Liang,Wanwen Chen,Yueheng Wu,Zhanyi Lin

Biological procedures online 27:14 PubMed40229695

2025

Differences in Fatty Acid Metabolism between MCDD and HFD Induced Metabolic Dysfunction-associated Fatty Liver Disease Model Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Xuan Wang,Xin-Zhu Liu,Zhen Guo,Hui-Lin Zhang,Li Qi,Jia Liu,Ping Liu,Guo-Xiang Xie,Xiao-Ning Wang

iScience 28:111752 PubMed39898049

2025

Extracellular vesicles contribute to the beneficial effects of exercise training in APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver K Fuller,Emma D McLennan,Casey L Egan,Nimna Perera,Lauren V Terry,Jae Pyun,Mariana de Mendonca,Guilherme Defante Telles,Benoit Smeuninx,Emma L Burrows,Ghizal Siddiqui,Darren J Creek,John W Scott,Michael A Pearen,Pamali Fonseka,Joseph A Nicolazzo,Suresh Mathivanan,Anthony J Hannan,Grant A Ramm,Martin Whitham,Mark A Febbraio

International journal of molecular sciences 25: PubMed39125704

2024

Inline Raman Spectroscopy Provides Versatile Molecular Monitoring for Platelet Extracellular Vesicle Purification with Anion-Exchange Chromatography.

Applications

Unspecified application

Species

Unspecified reactive species

Heikki Saari,Heli Marttila,Minna M Poranen,Hanna M Oksanen,Jacopo Zini,Saara Laitinen

Redox biology 75:103241 PubMed38901103

2024

Extracellular vesicles from II trimester human amniotic fluid as paracrine conveyors counteracting oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Senesi,Laura Guerricchio,Maddalena Ghelardoni,Nadia Bertola,Stefano Rebellato,Nicole Grinovero,Martina Bartolucci,Ambra Costa,Andrea Raimondi,Cristina Grange,Sara Bolis,Valentina Massa,Dario Paladini,Domenico Coviello,Assunta Pandolfi,Benedetta Bussolati,Andrea Petretto,Grazia Fazio,Silvia Ravera,Lucio Barile,Carolina Balbi,Sveva Bollini

Journal of extracellular vesicles 13:e12460 PubMed38853287

2024

Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Yang,Heng Zhang,Si Chen,Kaibin Lou,Meng Zhou,Mingchao Zhang,Rui Lu,Chunxia Zheng,Limin Li,Qihan Chen,Zhihong Liu,Ke Zen,Yanggang Yuan,Hongwei Liang

American journal of physiology. Gastrointestinal and liver physiology 326:G747-G761 PubMed38591148

2024

StarD5 levels of expression correlate with onset and progression of steatosis and liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Genta Kakiyama,Kei Minoiwa,Nanah Bai-Kamara,Taishi Hashiguchi,William M Pandak,Daniel Rodriguez-Agudo

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23522 PubMed38445789

2024

Microsomal triglyceride transfer protein is necessary to maintain lipid homeostasis and retinal function.

Applications

Unspecified application

Species

Unspecified reactive species

Catharina R Grubaugh,Anuradha Dhingra,Binu Prakash,Diego Montenegro,Janet R Sparrow,Lauren L Daniele,Christine A Curcio,Brent A Bell,M Mahmood Hussain,Kathleen Boesze-Battaglia

Journal of lipid research 65:100525 PubMed38417553

2024

Mining cholesterol genes from thousands of mouse livers identifies aldolase C as a regulator of cholesterol biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

James A Votava,Steven V John,Zhonggang Li,Shuyang Chen,Jing Fan,Brian W Parks

STAR protocols 4:102515 PubMed37742179

2023

Proteomics protocol for obtaining extracellular vesicle from human plasma using asymmetrical flow field-flow fractionation technology.

Applications

Unspecified application

Species

Unspecified reactive species

Na Li,Yumeng Yan,Bowen Wu,Jifeng Wang,Fuquan Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein B antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (99)

Product promise