AB231574

Anti-Apolipoprotein B antibody

Name: Anti-Apolipoprotein B antibody (ab231574) | Abcam
Brand: Abcam Limited
SKU: AB231574

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(3 Publications)

Rabbit Polyclonal Apolipoprotein B antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APOB aa 3350-3550.

View Alternative Names

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (AB231574)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (AB231574)

Formalin-fixed, paraffin-embedded human liver tissue stained for Apolipoprotein B using ab231574 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Apolipoprotein B antibody (AB231574)

All lanes:

Western blot - Anti-Apolipoprotein B antibody (ab231574) at 2 µg/mL

All lanes:

Pig liver lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 515 kDa

false

Supplier Data

Western blot - Anti-Apolipoprotein B antibody (AB231574)

All lanes:

Western blot - Anti-Apolipoprotein B antibody (ab231574) at 2 µg/mL

All lanes:

Recombinant human Apolipoprotein B protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 515 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human APOB aa 3350-3550. The exact immunogen used to generate this antibody is proprietary information.

P04114

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231574 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

See full target information APOB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed41041859

2025

CENPU promotes tumorigenesis and stem cell properties in triple‑negative breast cancer by suppressing lysosomal furin degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Shujuan Sun,Zhenyu Hou,Ling Qiang,Dongdong Zhou

Clinical and translational medicine 11:e482 PubMed34459123

2021

Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR-485-5p-mediated CXCL10 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yingwei Chen,Xiaojie Chen,Haiyu Li,Yunpeng Li,Dong Cheng,Yi Tang,Haiqiang Sang

Frontiers in oncology 11:654689 PubMed33954113

2021

Apolipoprotein B Is Associated With the Microenvironment of Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Xu,Diyu Chen,Xiaode Feng,Jiating Hu,Jiangzhen Ge,Chaobiao Yan,Deguo Zhang,Zhenan Ling,Jianzhong Chen,Jian Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (AB231574)

Western blot - Anti-Apolipoprotein B antibody (AB231574)

Western blot - Anti-Apolipoprotein B antibody (AB231574)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise