Anti-Apolipoprotein B antibody
4
(2 Reviews)
|
(21 Publications)
Goat Polyclonal Apolipoprotein B antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human APOB.
View Alternative Names
Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100
- WB
Unknown
Western blot - Anti-Apolipoprotein B antibody (AB7616)
All lanes:
Western blot - Anti-Apolipoprotein B antibody (ab7616) at 1 µg/mL
All lanes:
Western blot - Native Human Apolipoprotein B (<a href='/en-us/products/proteins-peptides/native-human-apolipoprotein-b-ab77859'>ab77859</a>) at 0.01 µg
Secondary
All lanes:
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Predicted band size: 515 kDa
true
Exposure time: 3min
- WB
CiteAb
Western blot - Anti-Apolipoprotein B antibody (AB7616)
Apolipoprotein B western blot using anti-Apolipoprotein B antibody ab7616. Publication image and figure legend from Shimizu, M., Li, J., et al., 2015, PLoS One, PubMed 25875015.
ab7616 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab7616 please see the product overview.
Quercetin repressed the expression of genes related to lipoprotein metabolism in Caco-2 cells.(A) Differentiated Caco-2 cells were treated with vehicle or 100 μM quercetin for 24 h, after which total RNA was isolated. mRNA levels, which were determined by quantitative real-time PCR, are presented as relative expression after normalization to 36B4 mRNA. Relative mRNA levels in vehicle-treated cells were set to 1. Data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01. (B) Differentiated Caco-2 cells were treated with vehicle (Veh) or 100 μM quercetin (Q) for 12 h. Proteins were extracted and immunoblots (IB) were performed. (C) Differentiated Caco-2 cells were exposed to vehicle or the indicated doses of quercetin for 24 h, after which total RNA was isolated. Data are presented as mean ± SD (n = 3). **p < 0.01.
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Reactivity data
Product details
This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.
Pathways
ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.
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Publications (21)
Recent publications for all applications. Explore the full list and refine your search
iScience 25:104542 PubMed35754737
2022
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Journal of dairy science 105:5420-5434 PubMed35469640
2022
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Journal of dairy science 105:3588-3600 PubMed35181144
2022
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PLoS pathogens 17:e1009889 PubMed34492079
2021
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The Journal of biological chemistry 297:100948 PubMed34252459
2021
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Journal of lipid research 62:100091 PubMed34118252
2021
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Journal of dairy science 103:10728-10741 PubMed32952018
2020
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Malaria journal 19:155 PubMed32295584
2020
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EBioMedicine 43:114-126 PubMed31047861
2019
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Journal of dairy science 102:833-845 PubMed30415861
2018
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