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AB7616

Anti-Apolipoprotein B antibody

4

(2 Reviews)

|

(21 Publications)

Goat Polyclonal Apolipoprotein B antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human APOB.

View Alternative Names

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

2 Images
Western blot - Anti-Apolipoprotein B antibody (AB7616)
  • WB

Unknown

Western blot - Anti-Apolipoprotein B antibody (AB7616)

All lanes:

Western blot - Anti-Apolipoprotein B antibody (ab7616) at 1 µg/mL

All lanes:

Western blot - Native Human Apolipoprotein B (<a href='/en-us/products/proteins-peptides/native-human-apolipoprotein-b-ab77859'>ab77859</a>) at 0.01 µg

Secondary

All lanes:

Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

Predicted band size: 515 kDa

true

Exposure time: 3min

Western blot - Anti-Apolipoprotein B antibody (AB7616)
  • WB

CiteAb

Western blot - Anti-Apolipoprotein B antibody (AB7616)

Apolipoprotein B western blot using anti-Apolipoprotein B antibody ab7616. Publication image and figure legend from Shimizu, M., Li, J., et al., 2015, PLoS One, PubMed 25875015.

ab7616 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab7616 please see the product overview.

Quercetin repressed the expression of genes related to lipoprotein metabolism in Caco-2 cells.(A) Differentiated Caco-2 cells were treated with vehicle or 100 μM quercetin for 24 h, after which total RNA was isolated. mRNA levels, which were determined by quantitative real-time PCR, are presented as relative expression after normalization to 36B4 mRNA. Relative mRNA levels in vehicle-treated cells were set to 1. Data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01. (B) Differentiated Caco-2 cells were treated with vehicle (Veh) or 100 μM quercetin (Q) for 12 h. Proteins were extracted and immunoblots (IB) were performed. (C) Differentiated Caco-2 cells were exposed to vehicle or the indicated doses of quercetin for 24 h, after which total RNA was isolated. Data are presented as mean ± SD (n = 3). **p < 0.01.

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Native Full Length Protein corresponding to Human APOB.

P04114

Specificity

Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Reactivity data

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Product details

This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
Storage buffer
pH: 8 Preservative: 0.01% Sodium azide Constituents: 4.77% Sodium borate, 0.44% Sodium chloride, 0.15% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.
Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
See full target information APOB

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:104542 PubMed35754737

2022

Organoid-derived intestinal epithelial cells are a suitable model for preclinical toxicology and pharmacokinetic studies.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Takahashi,Makoto Noguchi,Yu Inoue,Shintaro Sato,Makoto Shimizu,Hirotatsu Kojima,Takayoshi Okabe,Hiroshi Kiyono,Yoshio Yamauchi,Ryuichiro Sato

Journal of dairy science 105:5420-5434 PubMed35469640

2022

Role of sortilin 1 (SORT1) on lipid metabolism in bovine liver.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Shuang Wang,Juan J Loor,Qianming Jiang,Changhong Gao,Mingmao Yang,Yan Tian,Wenwen Fan,Yingying Zhao,Bingbing Zhang,Chuang Xu

Journal of dairy science 105:3588-3600 PubMed35181144

2022

Role of diacylglycerol O-acyltransferase (DGAT) isoforms in bovine hepatic fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Shuang Wang,Juan J Loor,Matheus G Lopes,Yingying Zhao,Xinru Ma,Ming Li,Bingbing Zhang,Chuang Xu

PLoS pathogens 17:e1009889 PubMed34492079

2021

Hepatitis C virus induces oxidation and degradation of apolipoprotein B to enhance lipid accumulation and promote viral production.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Wang,Yue Zhu,Congci Yu,Chongyang Zhang,Qing Tang,He Huang,Zhendong Zhao

The Journal of biological chemistry 297:100948 PubMed34252459

2021

Recognition of lipoproteins by scavenger receptor class A members.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cheng,Enlin Zheng,Bowen Yu,Ze Zhang,Yuanyuan Wang,Yingbin Liu,Yongning He

Journal of lipid research 62:100091 PubMed34118252

2021

Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4.

Applications

Unspecified application

Species

Unspecified reactive species

Bingxiang Wang,Yishi Shen,Lei Zhai,Xiaodan Xia,Hong-Mei Gu,Maggie Wang,Yongfang Zhao,Xiaole Chang,Adekunle Alabi,Sijie Xing,Shijun Deng,Boyan Liu,Guiqing Wang,Shucun Qin,Da-Wei Zhang

Journal of dairy science 103:10728-10741 PubMed32952018

2020

Knockdown of phosphatase and tensin homolog (PTEN) inhibits fatty acid oxidation and reduces very low density lipoprotein assembly and secretion in calf hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Bichen Zhao,Chunhai Luo,Menglong Zhang,Feifei Xing,Shengbin Luo,Shixin Fu,Xudong Sun

Malaria journal 19:155 PubMed32295584

2020

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Tougan,Jyotheeswara R Edula,Masayuki Morita,Eizo Takashima,Hajime Honma,Takafumi Tsuboi,Toshihiro Horii

EBioMedicine 43:114-126 PubMed31047861

2019

Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Notarangelo,Chiara Zucal,Angelika Modelska,Isabella Pesce,Giorgina Scarduelli,Cristina Potrich,Lorenzo Lunelli,Cecilia Pederzolli,Paola Pavan,Giancarlo la Marca,Luigi Pasini,Paola Ulivi,Himisha Beltran,Francesca Demichelis,Alessandro Provenzani,Alessandro Quattrone,Vito G D'Agostino

Journal of dairy science 102:833-845 PubMed30415861

2018

Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Hongdou Jia,Xiaobing Li,Guowen Liu,Juan J Loor,Ryan Bucktrout,Xudong Sun,Guojin Li,Xin Shu,Jihong Dong,Yazhe Wang,Rankun Zuo,Zhe Wang,Xinwei Li
View all publications

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