AB312319

Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Apolipoprotein B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Apolipoprotein B-100, Apo B-100, Apob, Apolipoprotein B100

4 Images

Supplier Data

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

This data was developed using ab312318, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9837916, 10225972).

Negative control : Hela (PMID : 2501159).

The identity of the lower MW bands at approximately 300 kDa and 150 kDa is unknown.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (<a href='/en-us/products/primary-antibodies/apolipoprotein-b-antibody-epr27473-49-ab312318'>ab312318</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 509 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

This data was developed using ab312318, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9837916, 10225972).

Low expression : lung.

The identity of the lower MW band at approximately 150 kDa is unknown.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (<a href='/en-us/products/primary-antibodies/apolipoprotein-b-antibody-epr27473-49-ab312318'>ab312318</a>) at 1/1000 dilution

Lane 1:

Human serum lysate at 20 µg

Lane 2:

Human plasma lysate at 20 µg

Lane 3:

Human lu lysate at 20 µg

Lane 4:

Mouse serum lysate at 20 µg

Lane 5:

Mouse plasma lysate at 20 µg

Lane 6:

Mouse lu lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250 kDa,509 kDa

true

Exposure time: 180s

Lab

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

This data was developed using ab312318, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This product is unable to detect B-100 in liver samples due to sensitivity limitations.

The doublet observed in rat shows consistency with findings reported in PMID : 7670964 and PMID : 9837916

Lanes 1 - 3:

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (<a href='/en-us/products/primary-antibodies/apolipoprotein-b-antibody-epr27473-49-ab312318'>ab312318</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319) at 1/1000 dilution

Lane 1:

Rat serum lysate at 20 µg

Lane 2:

Rat plasma lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 509 kDa,250 kDa

false

Exposure time: 40s

Lab

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

This data was developed using ab312318, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This product is unable to detect B-100 in liver samples due to sensitivity limitations.

Antibody ab315265, which specifically targets ApoB-100, serves as an effective alternative for ApoB-100 detection.

Lane 1:

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (<a href='/en-us/products/primary-antibodies/apolipoprotein-b-antibody-epr27473-49-ab312318'>ab312318</a>) at 1/1000 dilution

Lane 2:

Western blot - Anti-Apolipoprotein B-100 antibody [EPR19946-175] (<a href='/en-us/products/primary-antibodies/apolipoprotein-b-100-antibody-epr19946-175-ab315265'>ab315265</a>) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250 kDa,509 kDa

false

Exposure time: 10s

Unconjugated

Anti-Apolipoprotein B antibody [EPR27473-49]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27473-49

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This product is unable to detect ApoB-100 in liver samples due to sensitivity limitations. Antibody <a href='/en-us/products/primary-antibodies/apolipoprotein-b-100-antibody-epr19946-175-ab315265'>ab315265</a>, which specifically targets ApoB-100, serves as an effective alternative for ApoB-100 detection.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

See full target information Apob

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise