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Rabbit Recombinant Monoclonal Apolipoprotein B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319), expandable thumbnail
  • Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319), expandable thumbnail
  • Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319), expandable thumbnail
  • Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (AB312319), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Species
Mouse
Dilution info
-
Notes

This product is unable to detect ApoB-100 in liver samples due to sensitivity limitations. Antibody Anti-Apolipoprotein B-100 antibody [EPR19946-175] ab315265, which specifically targets ApoB-100, serves as an effective alternative for ApoB-100 detection.

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Alternative names

Apolipoprotein B-100, Apo B-100, Apob

Recommended products

Rabbit Recombinant Monoclonal Apolipoprotein B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27473-49
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

Associated diseases and disorders

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319), expandable thumbnail

    Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319)

    Apolipoprotein B Western blot staining using rabbit Anti-Apolipoprotein B antibody

    This data was developed using Anti-Apolipoprotein B antibody [EPR27473-49] ab312318, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This product is unable to detect B-100 in liver samples due to sensitivity limitations.

    The doublet observed in rat shows consistency with findings reported in PMID: 7670964 and PMID: 9837916

    Lanes 1 - 3: Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (Anti-Apolipoprotein B antibody [EPR27473-49] ab312318) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319) at 1/1000 dilution

    Lane 1: Rat serum lysate at 20 µg

    Lane 2: Rat plasma lysate at 20 µg

    Lane 3: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 509 kDa, 250 kDa

    Exposure time: 40s

  • Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319), expandable thumbnail

    Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319)

    Apolipoprotein B Western blot staining using rabbit Anti-Apolipoprotein B antibody

    This data was developed using Anti-Apolipoprotein B antibody [EPR27473-49] ab312318, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This product is unable to detect B-100 in liver samples due to sensitivity limitations.

    Antibody Anti-Apolipoprotein B-100 antibody [EPR19946-175] ab315265, which specifically targets ApoB-100, serves as an effective alternative for ApoB-100 detection.

    Lane 1: Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (Anti-Apolipoprotein B antibody [EPR27473-49] ab312318) at 1/1000 dilution

    Lane 2: Western blot - Anti-Apolipoprotein B-100 antibody [EPR19946-175] (Anti-Apolipoprotein B-100 antibody [EPR19946-175] ab315265) at 1/1000 dilution

    All lanes: Mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 250 kDa, 509 kDa

    Exposure time: 10s

  • Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319), expandable thumbnail

    Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319)

    This data was developed using Anti-Apolipoprotein B antibody [EPR27473-49] ab312318, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9837916, 10225972).

    Negative control: Hela (PMID: 2501159).

    The identity of the lower MW bands at approximately 300 kDa and 150 kDa is unknown.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (Anti-Apolipoprotein B antibody [EPR27473-49] ab312318) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 509 kDa

    Exposure time: 180s

  • Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319), expandable thumbnail

    Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] - BSA and Azide free (ab312319)

    This data was developed using Anti-Apolipoprotein B antibody [EPR27473-49] ab312318, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9837916, 10225972).

    Low expression: lung.

    The identity of the lower MW band at approximately 150 kDa is unknown.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Apolipoprotein B antibody [EPR27473-49] (Anti-Apolipoprotein B antibody [EPR27473-49] ab312318) at 1/1000 dilution

    Lane 1: Human serum lysate at 20 µg

    Lane 2: Human plasma lysate at 20 µg

    Lane 3: Human lu lysate at 20 µg

    Lane 4: Mouse serum lysate at 20 µg

    Lane 5: Mouse plasma lysate at 20 µg

    Lane 6: Mouse lu lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 250, 509 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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