AB139401

Anti-Apolipoprotein B antibody [EPR2914]

RabMAb
Recombinant
What is this?

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(13 Publications)

Rabbit Recombinant Monoclonal Apolipoprotein B antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

2 Images

Unknown

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (AB139401)

All lanes:

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 515 kDa

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Lab

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (AB139401)

All lanes:

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilution

Lane 1:

Human plasma lysates at 20 µg

Lane 2:

Human serum lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 515 kDa

Observed band size: 515 kDa

false

Carrier free

Anti-Apolipoprotein B antibody [EPR2914] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2914

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

See full target information APOB

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70088 PubMed40511723

2025

Enhanced Early Detection of Colorectal Cancer via Blood Biomarker Combinations Identified Through Extracellular Vesicle Isolation and Artificial Intelligence Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Bonhan Koo,Young Il Kim,Minju Lee,Seok-Byung Lim,Yong Shin

Parasitology 152:381-394 PubMed40134311

2025

Characterization and microRNA quantification of plasma-derived extracellular vesicles in patients with infection.

Applications

Unspecified application

Species

Unspecified reactive species

Thunchanok Khammanee,Natakorn Nokchan,Piyatida Molika,Hansuk Buncherd,Kanitta Srinoun,Natta Tansila,Charinrat Saechan,Rachanida Praparatana,Nongyao Sawangjaroen,Suwannee Jitueakul,Chatree Ratcha,Churat Weeraphan,Jisnuson Svasti,Raphatphorn Navakanitworakul,Supinya Thanapongpichat

Journal of extracellular vesicles 13:e12486 PubMed39104279

2024

A Predictive Model for Initial Platinum-Based Chemotherapy Efficacy in Patients with Postoperative Epithelial Ovarian Cancer Using Tissue-Derived Small Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Shen,Conghui Wang,Jiaxin Gu,Feifei Song,Xiaodong Wu,Fangfang Qian,Xiaojing Chen,Lingfang Wang,Qiaohua Peng,Ziyu Xing,Lingkai Gu,Fenfen Wang,Xiaodong Cheng

Journal of extracellular vesicles 13:e12455 PubMed38887871

2024

Microglial activation induces nitric oxide signalling and alters protein S-nitrosylation patterns in extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha Vassileff,Jereme G Spiers,Sarah E Bamford,Rohan G T Lowe,Keshava K Datta,Paul J Pigram,Andrew F Hill

Molecular neurobiology 61:5901-5915 PubMed38252383

2024

A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha Vassileff,Jereme G Spiers,John D Lee,Trent M Woodruff,Esmaeil Ebrahimie,Manijeh Mohammadi Dehcheshmeh,Andrew F Hill,Lesley Cheng

Open medicine (Warsaw, Poland) 18:20230790 PubMed37711155

2023

Lumican silencing ameliorates β-glycerophosphate-mediated vascular smooth muscle cell calcification by attenuating the inhibition of APOB on KIF2C activity.

Applications

Unspecified application

Species

Unspecified reactive species

Haibin Li,Chunyan Zhang,Qiang Liu

International journal of molecular sciences 24: PubMed37628895

2023

Platelet-Derived Extracellular Vesicles as Lipid Carriers in Severe Allergic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Couto-Rodriguez,Alma Villaseñor,Carmela Pablo-Torres,David Obeso,María Fernanda Rey-Stolle,Héctor Peinado,José Luis Bueno,Mar Reaño-Martos,Alfredo Iglesias Cadarso,Cristina Gomez-Casado,Coral Barbas,Domingo Barber,María M Escribese,Elena Izquierdo

Science advances 9:eade0293 PubMed37235655

2023

Secreted endogenous macrosomes reduce Aβ burden and ameliorate Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Cunli Wang,Yiming Yang,Xiaoyu Zhang,Zhenqiang Shi,Huiling Gao,Manli Zhong,Yonggang Fan,Hongyan Zhang,Bo Liu,Guangyan Qing

Communications biology 5:1358 PubMed36496485

2022

Electrodeposited magnetic nanoporous membrane for high-yield and high-throughput immunocapture of extracellular vesicles and lipoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Zhang,Xiaoye Huo,Yini Zhu,James N Higginbotham,Zheng Cao,Xin Lu,Jeffrey L Franklin,Kasey C Vickers,Robert J Coffey,Satyajyoti Senapati,Ceming Wang,Hsueh-Chia Chang

Journal of extracellular biology 1:e66 PubMed38939906

2022

Comparative and integrated analysis of plasma extracellular vesicle isolation methods in healthy volunteers and patients following myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Daan Paget,Antonio Checa,Benedikt Zöhrer,Raphael Heilig,Mayooran Shanmuganathan,Raman Dhaliwal,Errin Johnson,Maléne Møller Jørgensen,Rikke Bæk,Craig E Wheelock,Keith M Channon,Roman Fischer,Daniel C Anthony,Robin P Choudhury,Naveed Akbar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein B antibody [EPR2914]

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (AB139401)

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (AB139401)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise