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AB313868

Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free

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Rabbit Recombinant Monoclonal Apolipoprotein B antibody. Carrier free. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant fragment, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human APOB.

View Alternative Names

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Apolipoprotein B with ab313868 at 1/500 dilution (green). Blue : Fluoroshield with DAPI. Scale bar= 10um.

Indirect ELISA - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

Indirect ELISA analysis was performed by coating the plate with purified native human ApoB protein (62.5-4000 ng/mL). Coated protein was probed with ab313868 at 1 ug/mL. Goat anti-rabbit IgG antibody at 1/10000 dilution was used to detect the bound primary antibody.

Western blot - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)
  • WB

Supplier Data

Western blot - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

All lanes:

Western blot - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (ab313868) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 516 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1962

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

I-ELISA, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human APOB.

P04114

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.
Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
See full target information APOB
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Product promise

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