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Rabbit Recombinant Monoclonal Apolipoprotein CI/Apo-CI antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 11 publications.

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Images

Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (AB198288), expandable thumbnail
  • Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (AB198288), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (AB198288), expandable thumbnail
  • Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (AB198288), expandable thumbnail
  • Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (AB198288), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/40
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/800
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Inhibitor of lipoprotein binding to the low density lipoprotein (LDL) receptor, LDL receptor-related protein, and very low density lipoprotein (VLDL) receptor. Associates with high density lipoproteins (HDL) and the triacylglycerol-rich lipoproteins in the plasma and makes up about 10% of the protein of the VLDL and 2% of that of HDL. Appears to interfere directly with fatty acid uptake and is also the major plasma inhibitor of cholesteryl ester transfer protein (CETP). Binds free fatty acids and reduces their intracellular esterification. Modulates the interaction of APOE with beta-migrating VLDL and inhibits binding of beta-VLDL to the LDL receptor-related protein.

Alternative names

Apolipoprotein C-I, Apo-CI, ApoC-I, Apolipoprotein C1, APOC1

Recommended products

Rabbit Recombinant Monoclonal Apolipoprotein CI/Apo-CI antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16813
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Apolipoprotein CI (Apo-CI) plays a mechanical role in lipid metabolism where it acts as an inhibitor of lipoprotein lipase and hepatic lipase hindering lipolytic activity. It is also known as 'apo 250' and 'apo atv20' with a molecular mass of approximately 6.6 kDa. Apo-CI is primarily expressed in the liver and secreted into the plasma where it associates with high-density lipoproteins (HDL) and very low-density lipoproteins (VLDL).

Biological function summary

Apo-CI influences plasma triglyceride levels and cholesterol homeostasis. It plays a role in lipid transport and metabolism by modulating the binding of other apolipoproteins such as apo A-IV and interfering with the binding of lipoproteins to their receptors. Apo-CI is part of a complex of apolipoproteins that regulate lipid metabolism working in tandem to balance lipid and cholesterol levels in the body.

Pathways

Apo-CI is actively involved in the lipoprotein metabolism pathway and cholesterol efflux pathway. These pathways are important in maintaining lipid balance and cardiovascular health. Apo-CI is related to other apolipoproteins within these pathways such as apo E and apo A-I. Its function in modulating lipoprotein receptor interactions aligns with the broader mechanisms of these pathways in lipid and cholesterol regulation.

Associated diseases and disorders

Elevated levels of Apo-CI are associated with hyperlipidemia and cardiovascular disease. These conditions may result from its inhibitory effects on lipase activity leading to increased triglyceride levels. Apo-CI's interaction with other apolipoproteins such as apo B and apo E is linked to its role in the pathogenesis of these disorders. Understanding Apo-CI's involvement in these diseases can guide therapeutic strategies aimed at regulating its expression and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288), expandable thumbnail

    Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288) at 1/1000 dilution

    All lanes: Human fetal liver lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 9 kDa

    Observed band size: 6.6 kDa

  • Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288), expandable thumbnail

    Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288) at 1/5000 dilution

    All lanes: Human plasma lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 9 kDa

    Observed band size: 6.6 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288), expandable thumbnail

    Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Immunoprecipitation analysis of Human plasma whole cell extract labeling Apolipoprotein CI/Apo-CI using ab198288 at 1/40 dilution (Lane 2).
    Lane 3: IP using Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab198288 in Human plasma whole cell extract.
    Lane 1: Input: 10 μg Human plasma whole cell extract.
    Subsequent WB detection was performed using ab198288 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

    All lanes: Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Predicted band size: 9 kDa

    Observed band size: 6.6 kDa

  • Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288), expandable thumbnail

    Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Blocking/dilution buffer: 5% NFDM/TBST.

    We suggest treating cells with BFA which inhibits protein secretion.

    All lanes: Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288) at 1/1000 dilution

    Lanes 1 and 3: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 2 and 4: HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 300ng/ml BFA for 48 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 9 kDa

    Observed band size: 88 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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