AB21032

Anti-Apolipoprotein CIII antibody

Name: Anti-Apolipoprotein CIII antibody (ab21032) | Abcam
Brand: Abcam Limited
SKU: AB21032
Price: 605 USD
Availability: InStock

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(5 Publications)

Anti-Apolipoprotein CIII antibody (ab21032) is a rabbit polyclonal antibody detecting Apolipoprotein CIII in Western Blot, ICC/IF, ELISA. Suitable for Human, Monkey.

- Trusted since 2006

View Alternative Names

Apolipoprotein C-III, Apo-CIII, ApoC-III, Apolipoprotein C3, APOC3

2 Images

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Western blot - Anti-Apolipoprotein CIII antibody (AB21032)

All lanes:

Western blot - Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/mL

Lane 1:

Native Human Apolipoprotein CIII (ab77944) at 0.1 µg

Lane 2:

Native Human Apolipoprotein CIII (ab77944) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 11 kDa

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Exposure time: 1min

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CIII antibody (AB21032)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CIII antibody (AB21032)

ICC/IF image of ab21032 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells.

The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa-Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa-Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.

DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Monkey

Applications

ELISA, WB, sELISA, ICC/IF

applications

Immunogen

Full Length Protein corresponding to Human Apolipoprotein C-III. The exact immunogen used to generate this antibody is proprietary information.

P02656

Specificity

Specifically binds to human Apo CIII.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Apolipoprotein CIII antibody (ab21032) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Monkey samples.

What is the molecular weight of Apolipoprotein CIII?
Anti-Apolipoprotein CIII (ab21032) specifically detects a band for Apolipoprotein CIII (UniProt: P02656) at a molecular weight of 11kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified by Human Apo CIII-Sepharose affinity column.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.435% Sodium chloride, 0.0292% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein CIII also known as Apo C-III or ApoC3 is an apolipoprotein with a molecular mass of approximately 8.8 kDa. It is synthesized in the liver and intestine and circulates in the bloodstream primarily as part of very low-density lipoproteins (VLDL) and high-density lipoproteins (HDL). Its main role involves the regulation of lipid metabolism particularly in relation to triglyceride-rich particles. Apo C-III serves as an inhibitor of lipoprotein lipase an enzyme essential for the hydrolysis of triglycerides into free fatty acids.

Biological function summary

Apolipoprotein CIII plays a pivotal role in lipid and lipoprotein metabolism. It is not part of a large protein complex but interacts with lipoproteins as a modulatory protein. Apo C-III affects triglyceride metabolism by delaying the clearance of VLDL and enhancing hepatic triglyceride secretion. Additionally it modulates the uptake of lipoprotein remnants by liver receptors thereby influencing the plasma levels of triglycerides and cholesterol.

Pathways

Apolipoprotein CIII is involved in the regulation of lipid metabolic pathways. It plays a role in the lipoprotein assembly pathway and the triglyceride biosynthetic process. This protein impacts the interaction between lipoprotein lipase and its substrates hindering enzyme activity which leads to elevated plasma triglyceride levels. It relates closely to other apolipoproteins such as apolipoprotein E which also participates in lipid transport and metabolism although with distinct functions and regulatory mechanisms.

Apolipoprotein CIII is implicated in conditions like hypertriglyceridemia and cardiovascular disease. Elevated levels of Apo C-III contribute to high triglyceride levels promoting atherosclerosis development due to the retention of triglyceride-rich lipoproteins in the bloodstream. This association links it to cardiovascular disease risk where its role contrasts with proteins like apolipoprotein AI which is mainly involved in protective effects via HDL metabolism. Understanding the modulation of Apo C-III levels offers potential therapeutic avenues for managing these lipid-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma (PubMed : 18201179, PubMed : 22510806). Plays a multifaceted role in triglyceride homeostasis (PubMed : 18201179, PubMed : 22510806). Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs) (PubMed : 18201179, PubMed : 22510806). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors (PubMed : 18201179, PubMed : 22510806). Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners (PubMed : 18408013).

See full target information Apolipoprotein C-III

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

European journal of pharmacology 828:60-66 PubMed29555504

2018

Alantolactone suppresses APOC3 expression and alters lipid homeostasis in L02 liver cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meiting Yang,Hanhan Zhao,Huihan Ai,Hongbin Zhu,Shuyue Wang,Yongli Bao,Yuxin Li

Nature medicine 23:1086-1094 PubMed28825717

2017

A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels.

Applications

Unspecified application

Species

Unspecified reactive species

Sumeet A Khetarpal,Xuemei Zeng,John S Millar,Cecilia Vitali,Amritha Varshini Hanasoge Somasundara,Paolo Zanoni,James A Landro,Nicole Barucci,William J Zavadoski,Zhiyuan Sun,Hans de Haard,Ildikó V Toth,Gina M Peloso,Pradeep Natarajan,Marina Cuchel,Sissel Lund-Katz,Michael C Phillips,Alan R Tall,Sekar Kathiresan,Paul DaSilva-Jardine,Nathan A Yates,Daniel J Rader

Proceedings of the National Academy of Sciences of 109:9893-8 PubMed22566642

2012

Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells.

Applications

Species

Human

Katrine T-B G Schjoldager,Sergey Y Vakhrushev,Yun Kong,Catharina Steentoft,Aaron S Nudelman,Nis B Pedersen,Hans H Wandall,Ulla Mandel,Eric P Bennett,Steven B Levery,Henrik Clausen

Neuroscience research 73:161-7 PubMed22426495

2012

Identification and quantification of differentially expressed proteins in plasma of spinocerebellar ataxia type 12.

Applications

Unspecified application

Species

Unspecified reactive species

Vishnu Swarup,Achal K Srivastava,Moganty R Rajeswari

Journal of lipid research 52:1265-71 PubMed21371998

2011

Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum.

Applications

sELISA

Species

Cynomolgus monkey

Yuxin Wang,Zhili Song,Janice D Wagner,Catherine Pachuk,Romesh R Subramanian

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Apolipoprotein CIII antibody

Western blot - Anti-Apolipoprotein CIII antibody (AB21032)

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CIII antibody (AB21032)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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