AB51015

Anti-Apolipoprotein E antibody [EP1373Y]

20ul selling size
RabMAb
Recombinant
KO Validated
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(5 Publications)

Rabbit Recombinant Monoclonal Apolipoprotein E antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Apolipoprotein E, Apo-E, APOE

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Apolipoprotein E with purified ab51015 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Flow Cytometry (Intracellular) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Intracellular Flow Cytometry analysis of 293T cells labelling Apolipoprotein E with purified ab51015 at 1/20 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling Apolipoprotein with unpurified ab51015 at 1/100.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Apolipoprotein E with purified ab51015 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/100) and secondary antibody ab150120 an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Lab

Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

ab51015 (purified) at 1/30 immunoprecipitating Apolipoprotein in human fetal liver tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Predicted band size: 36 kDa

Observed band size: 36 kDa

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Unknown

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/10000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labelled at 1/2000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Lab

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Lab

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/10000 dilution

All lanes:

Human serum at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Lab

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

False colour image of Western blot : Anti-Apolipoprotein E antibody [EP1373Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab51015 was shown to bind specifically to Apolipoprotein E. A band was observed at 32-35 kDa in wild-type HepG2 cell lysates with no signal observed at this size in APOE knockout cell line ab280758 (knockout cell lysate ab282991). To generate this image, wild-type and APOE knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/1000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

APOE knockout HepG2 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 36 kDa

false

775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Apolipoprotein E antibody [EP1373Y]
Biotin

Biotin Anti-Apolipoprotein E antibody [EP1373Y]
Carrier free

Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free
660 APC

APC Anti-Apolipoprotein E antibody [EP1373Y]
HRP

HRP Anti-Apolipoprotein E antibody [EP1373Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Apolipoprotein E antibody [EP1373Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Apolipoprotein E antibody [EP1373Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Apolipoprotein E antibody [EP1373Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Apolipoprotein E antibody [EP1373Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Apolipoprotein E antibody [EP1373Y]
578 PE

PE Anti-Apolipoprotein E antibody [EP1373Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1373Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "For unpurified use at 1/70.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/20000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "For unpurified use at 1/30. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.

Biological function summary

ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.

Pathways

In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.

In Alzheimer’s disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids (PubMed : 14754908, PubMed : 1911868, PubMed : 6860692). APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance (PubMed : 14754908, PubMed : 1911868, PubMed : 1917954, PubMed : 23620513, PubMed : 2762297, PubMed : 6860692, PubMed : 9395455). Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma (PubMed : 2762297, PubMed : 6860692, PubMed : 9395455). As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) (PubMed : 1911868, PubMed : 6860692). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (PubMed : 12950167, PubMed : 1530612, PubMed : 1917954, PubMed : 20030366, PubMed : 20303980, PubMed : 2063194, PubMed : 2762297, PubMed : 7635945, PubMed : 7768901, PubMed : 8756331, PubMed : 8939961). Finally, APOE also has a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed : 23676495, PubMed : 7635945, PubMed : 9395455, PubMed : 9488694). A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes (PubMed : 1911868, PubMed : 1917954, PubMed : 23676495, PubMed : 29516132, PubMed : 9395455). APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues (PubMed : 2762297, PubMed : 29516132). By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis (PubMed : 1917954, PubMed : 2762297, PubMed : 29516132). APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis (PubMed : 14754908, PubMed : 23620513, PubMed : 9395455). First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues (PubMed : 14754908, PubMed : 23620513). Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes (PubMed : 9395455). APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting (PubMed : 25173806, PubMed : 8939961). APOE is also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells (By similarity). Binds to the immune cell receptor LILRB4 (PubMed : 30333625). APOE may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP (PubMed : 28111074).. (Microbial infection) Through its interaction with HCV envelope glycoprotein E2, participates in the attachment of HCV to HSPGs and other receptors (LDLr, VLDLr, and SR-B1) on the cell surface and to the assembly, maturation and infectivity of HCV viral particles (PubMed : 25122793, PubMed : 29695434). This interaction is probably promoted via the up-regulation of cellular autophagy by the virus (PubMed : 29695434).

See full target information APOE

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70172 PubMed39810624

2025

Single-cell RNA-sequencing and spatial transcriptomic analysis reveal a distinct population of APOE cells yielding pathological lymph node metastasis in papillary thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guohui Xiao,Rongli Xie,Jianhua Gu,Yishu Huang,Min Ding,Dongjie Shen,Jiqi Yan,Jianming Yuan,Qiong Yang,Wen He,Siyu Xiao,Haizhen Chen,Dan Xu,Jian Wu,Jian Fei

F1000Research 12:810 PubMed38161428

2024

Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation.

Applications

Species

Human

Riham Ayoubi,Kathleen Southern,Carl Laflamme

Journal of virology 96:e0209221 PubMed34935435

2021

The Host Factor ANP32A Is Required for Influenza A Virus vRNA and cRNA Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin E Nilsson-Payant,Benjamin R tenOever,Aartjan J W Te Velthuis

PloS one 15:e0227667 PubMed31978088

2020

Redefining transcriptional regulation of the APOE gene and its association with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Gyung Lee,Jessica Tulloch,Sunny Chen,Lesley Leong,Aleen D Saxton,Brian Kraemer,Martin Darvas,C Dirk Keene,Andrew Shutes-David,Kaitlin Todd,Steve Millard,Chang-En Yu

Cell metabolism 26:719-737.e6 PubMed28965825

2017

The Glia-Neuron Lactate Shuttle and Elevated ROS Promote Lipid Synthesis in Neurons and Lipid Droplet Accumulation in Glia via APOE/D.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Liu,Kevin R MacKenzie,Nagireddy Putluri,Mirjana Maletić-Savatić,Hugo J Bellen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein E antibody [EP1373Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Flow Cytometry (Intracellular) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (AB51015)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise