Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Apolipoprotein E with purified ab51015 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 293T cells labelling Apolipoprotein E with purified ab51015 at 1/20 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling Apolipoprotein with unpurified ab51015 at 1/100.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Apolipoprotein E with purified ab51015 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

• IP

Lab

Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

ab51015 (purified) at 1/30 immunoprecipitating Apolipoprotein in human fetal liver tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-ep1373y-ab51015'>ab51015</a>)

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Unknown

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-ep1373y-ab51015'>ab51015</a>) at 1/10000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labelled at 1/2000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Lab

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-ep1373y-ab51015'>ab51015</a>) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Lab

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-ep1373y-ab51015'>ab51015</a>) at 1/10000 dilution

All lanes:

Human serum at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Lab

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (AB171357)

This data was developed using ab51015, the same antibody clone in a different buffer formulation. False colour image of Western blot : Anti-Apolipoprotein E antibody [EP1373Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab51015 was shown to bind specifically to Apolipoprotein E. A band was observed at 32-35 kDa in wild-type HepG2 cell lysates with no signal observed at this size in APOE knockout cell line ab280758 (knockout cell lysate ab282991). To generate this image, wild-type and APOE knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-ep1373y-ab51015'>ab51015</a>) at 1/1000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

APOE knockout HepG2 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 36 kDa

false