Anti-Apolipoprotein E antibody [EPR19378]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophages of rat spleen is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of rat cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from APOE knockout mice staining with ab183596 at 1/10000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The section was incubated with ab183596 for 30 mins at room temperature. Positive staining on Brown (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from APOE knockout mice. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID : T001458).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Immunohistochemical analysis of paraffin-embedded (A) Brain tissue from wild-type C57BL/6JGpt mice and (B) Brian tissue from APOE knockout mice staining with ab183596 at 1/10000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. The section was incubated with ab183596 for 30 mins at room temperature.Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on Brown (A) Brain tissue from wild-type C57BL/6JGpt mice and no staining on (B) Brian tissue from APOE knockout mice.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID : T001458)

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse cortex tissue section labeling Apolipoprotein E with ab183596 at 1/500 dilution followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. The result showed cytoplasmic staining on mouse cortex. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatocytes of mouse liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Apolipoprotein E was immunoprecipitated from 1mg of Mouse plasma with ab183596 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183596 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse plasma, 10μg (Input).

Lane 2 : ab183596 IP in Mouse plasma.

Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183596 in Mouse plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

Predicted band size: 36 kDa

false

• WB

Lab

Western blot - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID : T001458).

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596) at 1/1000 dilution

Lane 1:

Wild-type mouse brain tissue lysate (male)

Lane 2:

APOE knockout mouse brain tissue lysate (male)

Lane 3:

Wild-type mouse liver tissue lysate (male)

Lane 4:

APOE knockout mouse liver tissue lysate (male)

Lane 5:

Wild-type mouse brain tissue lysate (female)

Lane 6:

APOE knockout mouse brain tissue lysate (female)

Lane 7:

Wild-type mouse liver tissue lysate (female)

Lane 8:

APOE knockout mouse liver tissue lysate (female)

false

• WB

Supplier Data

Western blot - Anti-Apolipoprotein E antibody [EPR19378] (AB183596)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2,3 and 4 : 4 seconds; Lane 5,6 and 7 : 15 seconds; Lane 8 : 1 minute.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596) at 1/5000 dilution

Lane 1:

Mouse plasma at 20 µg

Lane 2:

Mouse liver lysate at 20 µg

Lane 3:

Rat plasma at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Lane 5:

Mouse brain lysate at 20 µg

Lane 6:

Rat brain lysate at 20 µg

Lane 7:

Rat kidney lysate at 20 µg

Lane 8:

Mouse kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 21 kDa,36 kDa

Observed band size: 22 kDa,36 kDa

false