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AB245999

Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free

  • BOND RX™ Validated
  • KO Validated
  • RabMAb
  • Recombinant
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Apolipoprotein E antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Apolipoprotein E, Apo-E, Apoe

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophages of rat spleen is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of rat cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from APOE knockout mice staining with ab183596 at 1/10000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The section was incubated with ab183596 for 30 mins at room temperature. Positive staining on Brown (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from APOE knockout mice. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID : T001458).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Brain tissue from wild-type C57BL/6JGpt mice and (B) Brian tissue from APOE knockout mice staining with ab183596 at 1/10000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. The section was incubated with ab183596 for 30 mins at room temperature.Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on Brown (A) Brain tissue from wild-type C57BL/6JGpt mice and no staining on (B) Brian tissue from APOE knockout mice.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cortex tissue section labeling Apolipoprotein E with ab183596 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. The result showed cytoplasmic staining on mouse cortex. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatocytes of mouse liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • IP

Supplier Data

Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation.

Apolipoprotein E was immunoprecipitated from 1mg of Mouse plasma with ab183596 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183596 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse plasma, 10μg (Input).
Lane 2 : ab183596 IP in Mouse plasma.
Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183596 in Mouse plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-epr19378-ab183596'>ab183596</a>)

Predicted band size: 36 kDa

false

Western blot - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • WB

Lab

Western blot - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID : T001458).

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EPR19378] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-epr19378-ab183596'>ab183596</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse brain tissue lysate (male)

Lane 2:

APOE knockout mouse brain tissue lysate (male)

Lane 3:

Wild-type mouse liver tissue lysate (male)

Lane 4:

APOE knockout mouse liver tissue lysate (male)

Lane 5:

Wild-type mouse brain tissue lysate (female)

Lane 6:

APOE knockout mouse brain tissue lysate (female)

Lane 7:

Wild-type mouse liver tissue lysate (female)

Lane 8:

APOE knockout mouse liver tissue lysate (female)

false

Western blot - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)
  • WB

Supplier Data

Western blot - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (AB245999)

This data was developed using ab183596, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-4 : 4 seconds; Lanes 5-7 : 15 seconds; Lane 8 : 1 minute.

All lanes:

Western blot - Anti-Apolipoprotein E antibody [EPR19378] (<a href='/en-us/products/primary-antibodies/apolipoprotein-e-antibody-epr19378-ab183596'>ab183596</a>) at 1/5000 dilution

Lane 1:

Mouse plasma at 20 µg

Lane 2:

Mouse liver lysate at 20 µg

Lane 3:

Rat plasma at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Lane 5:

Mouse brain lysate at 20 µg

Lane 6:

Rat brain lysate at 20 µg

Lane 7:

Rat kidney lysate at 20 µg

Lane 8:

Mouse kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 21 kDa,36 kDa

Observed band size: 22 kDa,36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19378

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IHC-Fr, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Not suitable for testing cell lines in WB.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab245999 is the carrier-free version of ab183596.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.
Biological function summary

ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.

Pathways

In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.

In Alzheimer’s disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (By similarity). Finally, APOE also has a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed : 23676495).
See full target information Apoe
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering and regenerative medicine 16:515-529 PubMed35278347

2022

Transposon-mediated glial cell line-derived neurotrophic factor overexpression in human adipose tissue-derived mesenchymal stromal cells: A potential approach for neuroregenerative medicine?

Applications

Unspecified application

Species

Unspecified reactive species

Justyna Rasińska,Charlotte Klein,Laura Stahn,Felix Maidhof,Anna Pfeffer,Stefanie Schreyer,Manfred Gossen,Andreas Kurtz,Barbara Steiner,Shabnam Hemmati-Sadeghi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com