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AB231523

Anti-Apolipoprotein L1/APOL1 antibody

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(2 Publications)

Rabbit Polyclonal Apolipoprotein L1/APOL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APOL1 aa 1-250.

View Alternative Names

APOL, APOL1, Apolipoprotein L1, Apolipoprotein L, Apolipoprotein L-I, Apo-L, ApoL, ApoL-I

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody (AB231523)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody (AB231523)

Paraffin-embedded human kidney tissue stained for Apolipoprotein L 1/APOL1 using ab231523 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Apolipoprotein L1/APOL1 antibody (AB231523)
  • WB

Supplier Data

Western blot - Anti-Apolipoprotein L1/APOL1 antibody (AB231523)

All lanes:

Western blot - Anti-Apolipoprotein L1/APOL1 antibody (ab231523) at 3 µg/mL

All lanes:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 15 kDa,44 kDa,49 kDa

false

Western blot - Anti-Apolipoprotein L1/APOL1 antibody (AB231523)
  • WB

Supplier Data

Western blot - Anti-Apolipoprotein L1/APOL1 antibody (AB231523)

All lanes:

Western blot - Anti-Apolipoprotein L1/APOL1 antibody (ab231523) at 5 µg/mL

All lanes:

Recombinant human Apolipoprotein L 1/APOL1 protein

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human APOL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O14791

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231523 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein L1 also known as APOL1 is a protein with a mass of approximately 42 kDa. It is primarily expressed in the liver but can also be found in various tissues including the placenta pancreas and kidney. As a part of the apolipoprotein family APOL1 contributes to lipid binding and transport processes. The protein is involved in innate immunity due to its ability to bind and kill certain trypanosomes which are parasites that can cause disease in humans.
Biological function summary

APOL1 functions as a component in lipid transport and immune response. It plays an important role in the lytic pathway against trypanosomes by forming a pore in their lysosomal membranes leading to cell death. While it does not form part of a larger complex its activity is important when interacting with other factors and lipids. APOL1's actions demonstrate an integral aspect of the body's defense system particularly with its influence on cellular structures through lipid interactions.

Pathways

APOL1 is involved in the regulation of lipid metabolism and immune response pathways. It plays a role in the high-density lipoprotein (HDL) particle remodeling pathway. Another significant pathway involving APOL1 is the APOL family protein pathway which includes related proteins like APOL2 and APOL3. These proteins collaboratively contribute to intracellular lipid transport and immune defense establishing an interconnected network among them.

There is a significant connection between APOL1 variants and chronic kidney disease (CKD) and non-diabetic forms of End-Stage Renal Disease (ESRD). The risk alleles of the APOL1 gene are associated with increased incidence of CKD especially in individuals of African descent. These associations highlight the interaction between APOL1 and renal pathology as well as its linkage to other proteins like MYH9 which also relate to kidney diseases. Understanding APOL1's role in these conditions may aid in developing targeted medical interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
See full target information APOL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed34499625

2021

Recipient APOL1 risk alleles associate with death-censored renal allograft survival and rejection episodes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyang Zhang,Zeguo Sun,Jia Fu,Qisheng Lin,Khadija Banu,Kinsuk Chauhan,Marina Planoutene,Chengguo Wei,Fadi Salem,Zhengzi Yi,Ruijie Liu,Paolo Cravedi,Haoxiang Cheng,Ke Hao,Philip J O'Connell,Shuta Ishibe,Weijia Zhang,Steven G Coca,Ian W Gibson,Robert B Colvin,John Cijiang He,Peter S Heeger,Barbara Murphy,Madhav C Menon

Journal of vascular research 55:255-267 PubMed30179877

2018

Perfusion Tissue Culture Initiates Differential Remodeling of Internal Thoracic Arteries, Radial Arteries, and Saphenous Veins.

Applications

Unspecified application

Species

Unspecified reactive species

David A Prim,Vinal Menon,Shahd Hasanian,Laurel Carter,Tarek Shazly,Jay D Potts,John F Eberth
View all publications
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