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Rabbit Recombinant Monoclonal Apolipoprotein L1/APOL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.


Images

Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315), expandable thumbnail
  • Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

Function

May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.

Alternative names

APOL, APOL1, Apolipoprotein L1, Apolipoprotein L, Apolipoprotein L-I, Apo-L, ApoL, ApoL-I

Recommended products

Rabbit Recombinant Monoclonal Apolipoprotein L1/APOL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2907(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Apolipoprotein L1 also known as APOL1 is a protein with a mass of approximately 42 kDa. It is primarily expressed in the liver but can also be found in various tissues including the placenta pancreas and kidney. As a part of the apolipoprotein family APOL1 contributes to lipid binding and transport processes. The protein is involved in innate immunity due to its ability to bind and kill certain trypanosomes which are parasites that can cause disease in humans.

Biological function summary

APOL1 functions as a component in lipid transport and immune response. It plays an important role in the lytic pathway against trypanosomes by forming a pore in their lysosomal membranes leading to cell death. While it does not form part of a larger complex its activity is important when interacting with other factors and lipids. APOL1's actions demonstrate an integral aspect of the body's defense system particularly with its influence on cellular structures through lipid interactions.

Pathways

APOL1 is involved in the regulation of lipid metabolism and immune response pathways. It plays a role in the high-density lipoprotein (HDL) particle remodeling pathway. Another significant pathway involving APOL1 is the APOL family protein pathway which includes related proteins like APOL2 and APOL3. These proteins collaboratively contribute to intracellular lipid transport and immune defense establishing an interconnected network among them.

Associated diseases and disorders

There is a significant connection between APOL1 variants and chronic kidney disease (CKD) and non-diabetic forms of End-Stage Renal Disease (ESRD). The risk alleles of the APOL1 gene are associated with increased incidence of CKD especially in individuals of African descent. These associations highlight the interaction between APOL1 and renal pathology as well as its linkage to other proteins like MYH9 which also relate to kidney diseases. Understanding APOL1's role in these conditions may aid in developing targeted medical interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315), expandable thumbnail

    Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    All lanes: Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315) at 1/1000 dilution

    Lane 1: Human plasma lysate at 10 µg

    Lane 2: Human ovary cancer lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labeling Apolipoprotein L1/APOL1 with purified ab108315 at 1/50 dilution (9.2 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315), expandable thumbnail

    Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.

    Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.

    Lane 1:Human plasma

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    Predicted band size: 44 kDa

    Observed band size: 39 kDa, 42 kDa

  • Flow Cytometry (Intracellular) - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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