Rabbit Recombinant Monoclonal Apolipoprotein L1/APOL1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (AB252391), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-l1-apol1-antibody-eprbob-2-f1-1-bsa-and-azide-free-ab252391--immunoprecipitation-img144808.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (AB252391)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (AB252391), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-l1-apol1-antibody-eprbob-2-f1-1-bsa-and-azide-free-ab252391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72633.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (AB252391)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (AB252391), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-l1-apol1-antibody-eprbob-2-f1-1-bsa-and-azide-free-ab252391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13011.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (AB252391)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Alternative names
APOL, APOL1, Apolipoprotein L1, Apolipoprotein L, Apolipoprotein L-I, Apo-L, ApoL, ApoL-I
Recommended products
Rabbit Recombinant Monoclonal Apolipoprotein L1/APOL1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPRBOB-2-F1-1
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab252391 is the carrier-free version of Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein L1 also known as APOL1 is a protein with a mass of approximately 42 kDa. It is primarily expressed in the liver but can also be found in various tissues including the placenta pancreas and kidney. As a part of the apolipoprotein family APOL1 contributes to lipid binding and transport processes. The protein is involved in innate immunity due to its ability to bind and kill certain trypanosomes which are parasites that can cause disease in humans.
- Biological function summary
APOL1 functions as a component in lipid transport and immune response. It plays an important role in the lytic pathway against trypanosomes by forming a pore in their lysosomal membranes leading to cell death. While it does not form part of a larger complex its activity is important when interacting with other factors and lipids. APOL1's actions demonstrate an integral aspect of the body's defense system particularly with its influence on cellular structures through lipid interactions.
- Pathways
APOL1 is involved in the regulation of lipid metabolism and immune response pathways. It plays a role in the high-density lipoprotein (HDL) particle remodeling pathway. Another significant pathway involving APOL1 is the APOL family protein pathway which includes related proteins like APOL2 and APOL3. These proteins collaboratively contribute to intracellular lipid transport and immune defense establishing an interconnected network among them.
- Associated diseases and disorders
There is a significant connection between APOL1 variants and chronic kidney disease (CKD) and non-diabetic forms of End-Stage Renal Disease (ESRD). The risk alleles of the APOL1 gene are associated with increased incidence of CKD especially in individuals of African descent. These associations highlight the interaction between APOL1 and renal pathology as well as its linkage to other proteins like MYH9 which also relate to kidney diseases. Understanding APOL1's role in these conditions may aid in developing targeted medical interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-l1-apol1-antibody-eprbob-2-f1-1-bsa-and-azide-free-ab252391--immunoprecipitation-img144808.jpg "Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391)")
Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391)
Apolipoprotein L 1/APOL1 was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: HepG2 whole cell lysate 10 μg (Input).
Lane 2: Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217 in HepG2 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217).
All lanes: Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] (Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217)
Predicted band size: 44 kDa
Observed band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-l1-apol1-antibody-eprbob-2-f1-1-bsa-and-azide-free-ab252391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72633.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Apolipoprotein L 1/APOL1 with Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on glomerulus and weak cytoplasmic staining on renal tubules (PMID: 21997392, PMID: 28724794, PMID: 27138898) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-l1-apol1-antibody-eprbob-2-f1-1-bsa-and-azide-free-ab252391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13011.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] - BSA and Azide free (ab252391)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Apolipoprotein L 1/APOL1 with Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in plasma in blood vessels is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-1] ab252217).
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