AB246865

Anti-Apolipoprotein O antibody

Name: Anti-Apolipoprotein O antibody (ab246865) | Abcam
Brand: Abcam Limited
SKU: AB246865
Price: 655 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Apolipoprotein O antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human APOO aa 100 to C-terminus.

View Alternative Names

FAM121B, MIC23, MIC26, My025, UNQ1866/PRO4302, APOO, MICOS complex subunit MIC26, Apolipoprotein O, MICOS complex subunit MIC23, Protein FAM121B

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein O antibody (AB246865)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein O antibody (AB246865)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Apolipoprotein O (green) using ab246865 at 4μg/ml in ICC/IF.

Unknown

Western blot - Anti-Apolipoprotein O antibody (AB246865)

All lanes:

Western blot - Anti-Apolipoprotein O antibody (ab246865) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human APOO aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BUR5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein O (ApoO) also known as mitochondrial apolipoprotein is a protein with a molecular mass of 23 kDa. It interacts with lipoproteins and plays a role in lipid metabolism. ApoO is highly expressed in human tissues including the liver heart and skeletal muscle. It associates with high-density lipoprotein (HDL) particles and has been detected in the mitochondria indicating a potential role in energy metabolism. Researchers have identified this protein as part of the apolipoprotein family which is involved in transporting lipids throughout the body.

Biological function summary

ApoO contributes to the regulation of lipid and energy homeostasis. It is not known to associate with a larger protein complex but it may influence mitochondrial functions due to its localization. ApoO shows potential roles in modulating oxidative stress and maintaining cellular energy through influencing lipid peroxidation and mitochondrial respiration. This suggests that ApoO might play a part in cellular protection against oxidative damage which is important for maintaining the health of cells particularly those with high energy demands like cardiac and muscle cells.

Pathways

ApoO affects the lipid metabolism and energy production pathways. This protein impacts the pathway involved in cholesterol efflux and mitochondrial function. ApoO's action overlaps with other apolipoproteins especially ApoA-I which is a critical component of HDL involved in cholesterol transport. By modulating these pathways ApoO may contribute to systemic lipid balance and influence HDL-related cholesterol pathways an essential process in maintaining cardiovascular health.

ApoO relates to cardiovascular diseases and mitochondrial dysfunction. Abnormalities in ApoO expression or function could contribute to dyslipidemia and heart disease as this protein impacts lipid metabolism and heart tissue energy levels. ApoA-I which partners with ApoO in the HDL complex also plays a significant role in cardiovascular health. Furthermore altered ApoO levels or function could link to mitochondrial disorders affecting mitochondrial energy production and oxidative stress leading to broader implications for diseases involving energy metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed : 25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed : 24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed : 16956892).

See full target information APOO

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:548 PubMed39238028

2024

A nanoscale visual exploration of the pathogenic effects of bacterial extracellular vesicles on host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjae Kang,Min Jeong Kim,Dokyung Jeong,Hyung-Jun Lim,Ga-Eun Go,Uidon Jeong,Eunyoung Moon,Hee-Seok Kweon,Nae-Gyu Kang,Seung Jin Hwang,Sung Hun Youn,Bo Kyoung Hwang,Doory Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Apolipoprotein O antibody

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein O antibody (AB246865)

Western blot - Anti-Apolipoprotein O antibody (AB246865)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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