JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB234638

Anti-APOPT1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal APOPT1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COA8 aa 1-150.

View Alternative Names

APOP1, APOPT1, C14orf153, COA8, Cytochrome c oxidase assembly factor 8, APOP-1

1 Images
Western blot - Anti-APOPT1 antibody (AB234638)
  • WB

Supplier Data

Western blot - Anti-APOPT1 antibody (AB234638)

All lanes:

Western blot - Anti-APOPT1 antibody (ab234638) at 1/500 dilution

Lane 1:

Mouse brain lysate

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Lane 3:

A549 (human lung carcinoma cell line) cell lysate

Lane 4:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human COA8 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q96IL0

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOPT1 also known as Apo-T1 or CCDC86 is a protein involved in the regulation of mitochondrial function. It has a molecular mass of approximately 22 kDa. APOPT1 is mainly expressed in the tissues with high energy demands such as the heart and liver making it significant for energy metabolism. The protein functions within the mitochondria where it participates in the regulation of oxidative phosphorylation and influences mitochondrial integrity.
Biological function summary

The protein plays a significant role in protecting cells from oxidative stress by maintaining the proper function of the electron transport chain. APOPT1 is not part of a larger complex but acts as a solitary operator within mitochondrial pathways. It helps ensure that energy production remains efficient preventing cellular damage through the accumulation of reactive oxygen species. This protective mechanism supports the survival of cells under stress conditions.

Pathways

APOPT1 has a notable role in the apoptosis and mitochondrial biogenesis pathways. It interacts with proteins like Cytochrome c (CYCS) and Apoptotic protease activating factor 1 (APAF1) which are important in the intrinsic pathway of apoptosis. APOPT1's activity within the mitochondrial biogenesis pathway supports the creation and maintenance of new mitochondria which is essential for energy balance and cellular health.

APOPT1 is linked to mitochondrial disorders particularly those affecting the nervous and muscular systems. These disorders often lead to symptoms such as muscle weakness and neurodegeneration. The protein has connections to Cytochrome c oxidase deficiency a condition causing impaired electron transport chain function. Studies suggest that mutations or dysfunctions in APOPT1 can lead to improved understanding of the pathogenesis and potential therapeutic targets for these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for cytochrome c complex (COX) IV assembly and function Protects COX assembly from oxidation-induced degradation, COX being the terminal component of the mitochondrial respiratory chain.
See full target information COA8
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35159267

2022

Effect of Withanolide A on 7-Ketocholesterol Induced Cytotoxicity in hCMEC/D3 Brain Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Soh,Wei-Yi Ong
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB130751

Anti-Villin antibody [SP145]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [SP145] (AB130751)

  • 5
  • 5
Primary Antibodies

AB247258

Anti-ZAP70 antibody [YE291] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [YE291] - BSA and Azide free (AB247258)

  • 0
  • 0
Primary Antibodies

AB234744

Anti-YME1L1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YME1L1 antibody (AB234744)

  • 0
  • 0
Primary Antibodies

AB174854

Anti-WNK1 antibody [EPR2743(3)]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-WNK1 antibody [EPR2743(3)] (AB174854)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com