AB220832

Anti-APP-1 antibody

Name: Anti-APP-1 antibody (ab220832) | Abcam
Brand: Abcam Limited
SKU: AB220832

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(2 Publications)

Rabbit Polyclonal APP-1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PABPC4 aa 350-450.

View Alternative Names

APP1, PABP4, PABPC4, Polyadenylate-binding protein 4, PABP-4, Poly(A)-binding protein 4, Activated-platelet protein 1, Inducible poly(A)-binding protein, APP-1, iPABP

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-APP-1 antibody (AB220832)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-APP-1 antibody (AB220832)

Immunofluorescent analysis of PFA fixed, triton X-100 permeabilized U-2 OS cells labeling APP-1 with ab220832 at 4 μg/mL (green).

Supplier Data

Western blot - Anti-APP-1 antibody (AB220832)

All lanes:

Western blot - Anti-APP-1 antibody (ab220832) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 71 kDa

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Unknown

Western blot - Anti-APP-1 antibody (AB220832)

All lanes:

Western blot - Anti-APP-1 antibody (ab220832) at 0.4 µg/mL

Lane 1:

U-2197 cell line

Lane 2:

HeLa cell line

Predicted band size: 71 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PABPC4 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q13310

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APP-1 also known as Amyloid precursor protein 1 plays a significant mechanical role in cellular processes. It is a type I transmembrane glycoprotein with a molecular weight of around 110-130 kDa. APP-1 is widely expressed in various tissues but its highest levels are found in the central nervous system particularly within neurons. The processing of APP-1 occurs through cleavage by multiple secretases leading to the production of several fragments with different physiological roles.

Biological function summary

The alternative name Amyloid precursor protein reflects its pivotal involvement in neuronal growth and repair. APP-1 contributes to synaptic formation and plasticity being a part of a larger protein complex on the cell surface. Beyond its neuronal functions it plays roles in neural adhesion by interacting with extracellular matrix proteins. APP-1's fragments like amyloid-beta become relevant in cellular signaling and synaptic maintenance further influencing neuronal health.

Pathways

Research indicates that APP-1 is integral to the amyloidogenic and non-amyloidogenic pathways. It is connected to enzymes such as alpha beta and gamma secretases which modulate its cleavage and influence its downstream pathways. Interaction with proteins like presenilin is critical for processing APP-1 within the amyloidogenic pathway. These pathways collectively impact neurite outgrowth and neural development essential for maintaining cognitive functions.

Scientific studies have linked APP-1 to Alzheimer's disease due to the accumulation of its cleavage fragment amyloid-beta. This component aggregates to form plaques contributing to neurodegeneration and cognitive decline. Besides Alzheimer's variations in the APP-1 gene relate to cerebral amyloid angiopathy where deposition in blood vessels impairs circulation. Its interaction with Tau protein another key player in neurodegenerative diseases further associates APP-1 with the pathophysiology of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds the poly(A) tail of mRNA (PubMed : 8524242). Binds to SMIM26 mRNA and plays a role in its post-transcriptional regulation (PubMed : 37009826). May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo (By similarity).

See full target information PABPC4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 296:100036 PubMed33410401

2021

The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Evan P Booy,Daniel Gussakovsky,Taegi Choi,Sean A McKenna

Nucleic acids research 46:11575-11591 PubMed30247708

2018

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Applications

Unspecified application

Species

Unspecified reactive species

Evan P Booy,Ewan Ks McRae,Peyman Ezzati,Taegi Choi,Daniel Gussakovsky,Sean A McKenna

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APP-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-APP-1 antibody (AB220832)

Western blot - Anti-APP-1 antibody (AB220832)

Western blot - Anti-APP-1 antibody (AB220832)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise