AB187142

Anti-APPBP1 antibody [EPR15732]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal APPBP1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 4 publications.

View Alternative Names

APPBP1, HPP1, NAE1, NEDD8-activating enzyme E1 regulatory subunit, Amyloid protein-binding protein 1, Proto-oncogene protein 1, APP-BP1

4 Images

Flow Cytometry (Intracellular) - Anti-APPBP1 antibody [EPR15732] (AB187142)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-APPBP1 antibody [EPR15732] (AB187142)

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling APPBP1 with ab187142 at 1/120 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Western blot - Anti-APPBP1 antibody [EPR15732] (AB187142)

All lanes:

Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Human fetal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa

false

Supplier Data

Western blot - Anti-APPBP1 antibody [EPR15732] (AB187142)

All lanes:

Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 10 µg

Lane 2:

Rat heart tissue lysate at 10 µg

Lane 3:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa

false

Supplier Data

Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (AB187142)

Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab187142 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)

Predicted band size: 60 kDa

false

Carrier free

Anti-APPBP1 antibody [EPR15732] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15732

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/120", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein 'APPBP1' also known as APP-binding protein 1 functions as part of cellular processes through its role in protein modification. APPBP1 forms a dimeric complex with Uba3 together activating NEDD8 which is a small ubiquitin-like modifier. This protein has a molecular weight of approximately 58 kDa and is widely expressed across various tissues including the brain and liver. Its comprehensive roles make it essential for normal cellular operations.

Biological function summary

APPBP1 interacts within a complex known as the NEDD8-activating enzyme. This complex is essential in the neddylation pathway influencing protein degradation through the ubiquitin-proteasome system. By conjugating NEDD8 to target proteins APPBP1 controls many processes such as cell cycle regulation signal transduction and transcription. It is of special interest in research involving cellular signaling because neddylation modifies the activity and stability of many substrate proteins involved in these critical cellular functions.

Pathways

The involvement of APPBP1 in ubiquitin-like protein pathways highlights its influence on cellular homeostasis. The protein is associated with the neddylation and ubiquitination pathways influencing other members such as cullin proteins involved in cell cycle control and other cellular processes. APPBP1-related interactions play a significant role in ensuring the timely degradation of specific proteins maintaining balance within the cell's regulatory mechanisms.

Abnormalities in APPBP1 activity have been linked to neurodegenerative diseases such as Alzheimer's disease and cancer. In Alzheimer's disease APPBP1 is related to the APP protein and altered neddylation might contribute to disease pathology through dysfunctional protein degradation pathways. In cancer disrupted neddylation can result in uncontrolled cell proliferation due to improper regulation of cell cycle pathways. Understanding the function of APPBP1 could provide insights into developing therapeutic strategies for these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development.

See full target information NAE1

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 19:377-392 PubMed36632463

2023

Neddylation of HER2 Inhibits its Protein Degradation and promotes Breast Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xia,Tumei Hu,Xiaoyue He,Yuan Liu,Cuifu Yu,Weiyao Kong,Yuning Liao,Daolin Tang,Jinbao Liu,Hongbiao Huang

Cell reports 38:110460 PubMed35235781

2022

Proteome-centric cross-omics characterization and integrated network analyses of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Qi Gong,Yi-Zhou Jiang,Chen Shao,Wen-Ting Peng,Ming-Wei Liu,Da-Qiang Li,Ben-Yu Zhang,Peng Du,Yin Huang,Fei-Fei Li,Mu-Yun Li,Zhao-Lian Han,Xi Jin,Ding Ma,Yi Xiao,Peng-Yuan Yang,Jun Qin,Zhi-Ming Shao,Weimin Zhu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21800 PubMed34324733

2021

Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability.

Applications

Unspecified application

Species

Unspecified reactive species

Dacai Xu,Jiawen Wu,Jinghong Chen,Liling Jiang,Juan Chen,Wenhao Bao,Xin Chen,Qianqian Yang,Xiaolan Zhang,Leyi Yao,Huabo Su,Jinbao Liu

Oncology letters 18:2724-2732 PubMed31404297

2019

An overactive neddylation pathway serves as a therapeutic target and MLN4924 enhances the anticancer activity of cisplatin in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zeng,Yong-Shuang Iv,Qi-Hua Pan,Yi-Guo Zhou,He Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APPBP1 antibody [EPR15732]

Flow Cytometry (Intracellular) - Anti-APPBP1 antibody [EPR15732] (AB187142)

Western blot - Anti-APPBP1 antibody [EPR15732] (AB187142)

Western blot - Anti-APPBP1 antibody [EPR15732] (AB187142)

Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (AB187142)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise