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AB151305

Anti-APPBP2 antibody

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(2 Publications)

Rabbit Polyclonal APPBP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APPBP2 aa 300 to C-terminus.

View Alternative Names

KIAA0228, PAT1, APPBP2, Amyloid protein-binding protein 2, Amyloid beta precursor protein-binding protein 2, Protein interacting with APP tail 1, APP-BP2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-APPBP2 antibody (AB151305)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-APPBP2 antibody (AB151305)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling APPBP2 with ab151305 antibody at 1/100 dilution. Bottom figure represents cells costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPBP2 antibody (AB151305)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPBP2 antibody (AB151305)

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft tissue labeling APPBP2 with ab151305 antibody at 1/100 dilution.

Western blot - Anti-APPBP2 antibody (AB151305)
  • WB

Unknown

Western blot - Anti-APPBP2 antibody (AB151305)

7.5% SDS PAGE

All lanes:

Western blot - Anti-APPBP2 antibody (ab151305) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human APPBP2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92624

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APPBP2 is also known as amyloid precursor protein-binding protein 2 or PAT1. This protein has a molecular weight of roughly 135 kDa. APPBP2 acts as an adaptor molecule and plays a mechanical role in intracellular transport processes. It interacts directly with microtubule-associated proteins facilitating vesicular transport along the cytoskeletal network. APPBP2 expression is found prominently in the brain and lymphocytes hinting at its significance in these areas.
Biological function summary

APPBP2 contributes to cellular processes by involving itself in the regulation of protein transport. It is part of a larger protein complex which includes kinesins that mediates cargo movement within cells. This action influences various cellular functions including cell signaling and neurotransmitter release. Through its interactions APPBP2 can affect synaptic activity by modulating the trafficking of synaptic vesicles and other cargo pertinent to neuronal communication.

Pathways

APPBP2 engages with the Wnt signaling pathway and neuronal apoptosis pathway. APPBP2 demonstrates a regulatory role by interacting with proteins such as beta-catenin in the Wnt pathway. These interactions influence gene expression and cell proliferation. In neuronal apoptosis APPBP2 acts in coordination with amyloid precursor protein (APP) further dictating cell survival and neurodegeneration processes.

APPBP2 has implications in Alzheimer's disease and lymphomas. In Alzheimer's disease APPBP2 affects the processing of amyloid precursor protein connecting it to the pathogenesis of amyloid plaques characteristic of this condition. In lymphomas altered expression levels of APPBP2 may interact with oncogenic proteins contributing to tumor progression. This involvement links APPBP2 to broader cellular pathways and emphasizes its role in pathological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed : 29775578, PubMed : 29779948, PubMed : 37844242). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed : 29775578, PubMed : 29779948, PubMed : 37844242). The CRL2(APPBP2) complex specifically recognizes proteins with a -Arg-Xaa-Xaa-Gly degron at the C-terminus, leading to their ubiquitination and degradation (PubMed : 29775578, PubMed : 29779948, PubMed : 37844242). The CRL2(APPBP2) complex mediates ubiquitination and degradation of truncated SELENOV selenoproteins produced by failed UGA/Sec decoding, which end with a -Arg-Xaa-Xaa-Gly degron (PubMed : 26138980, PubMed : 37844242). The CRL2(APPBP2) complex negatively regulates beige adipocyte differentiation by mediating ubiquitination and degradation of PRDM16 (By similarity). May play a role in intracellular protein transport : may be involved in the translocation of APP along microtubules toward the cell surface (PubMed : 9843960).
See full target information APPBP2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:6605-6628 PubMed32111743

2020

A kinesin adapter directly mediates dendritic mRNA localization during neural development in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Jing Zhou,Tianhui Zhu,Ivan Cohen,Jason Dictenberg

EBioMedicine 44:138-149 PubMed31105033

2019

APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyuan Gong,Fei Liu,Xiaoyu Liu,Shengping Min,Nan Wu,Xincheng Liu,Yueguang Liu,Sue Han,Yijie Zhang,Yuefang Zhang,Yudong Hu,Xuegang Liu,Xiaojing Wang
View all publications
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Product promise

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