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AB326696

Anti-APPL antibody [SR3775]

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Rabbit Recombinant Monoclonal APPL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

APPL, DIP13A, KIAA1428, APPL1, DCC-interacting protein 13-alpha, Dip13-alpha

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [SR3775] (AB326696)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [SR3775] (AB326696)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for APPL using antibody at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [SR3775] (AB326696)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [SR3775] (AB326696)

ICC/IF staining of APPL in HepG2 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Western blot - Anti-APPL antibody [SR3775] (AB326696)
  • WB

Supplier Data

Western blot - Anti-APPL antibody [SR3775] (AB326696)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : C2C12 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 80 kDa
Observed band size : 80 kDa

Exposure time : 5 seconds

All lanes:

Western blot - Anti-APPL antibody [SR3775] (ab326696) at 1/1000 dilution

Lane 1:

Hela whole cell lysates at 20 µg

Lane 2:

C2C12 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

Exposure time: 5s

Western blot - Anti-APPL antibody [SR3775] (AB326696)
  • WB

Supplier Data

Western blot - Anti-APPL antibody [SR3775] (AB326696)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : APPL Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-APPL antibody [SR3775] (ab326696) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

APPL Rabbit mAb knockdown Hela cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR3775

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism (PubMed : 10490823, PubMed : 15016378, PubMed : 19661063, PubMed : 26073777, PubMed : 26583432). Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (PubMed : 15016378). Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (By similarity). Regulates TLR4 signaling in activated macrophages (By similarity). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner (PubMed : 26583432). Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways (PubMed : 19661063, PubMed : 24879834, PubMed : 26073777). Required for fibroblast migration through HGF cell signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex (PubMed : 19433865).
See full target information APPL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com