Anti-Aprataxin antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aprataxin antibody (AB154413)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Aprataxin using ab154413 at a 1/500 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aprataxin antibody (AB154413)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human A549 xenograft tissue, labeling Aprataxin using ab154413 at a 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aprataxin antibody (AB154413)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labelling Aprataxin protein at nucleus with ab154413 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aprataxin antibody (AB154413)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labelling Aprataxin protein at nucleus with ab154413 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-Aprataxin antibody (AB154413)

False colour image of Western blot : Anti-Aprataxin antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab154413 was shown to bind specifically to Aprataxin. A band was observed at 41 kDa in wild-type HeLa cell lysates with no signal observed at this size in APTX knockout cell line ab265118 (knockout cell lysate ab257837). The band observed in the knockout lysate lane below 41 kDa is likely to represent a truncated form of Aprataxin. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and APTX knockout HeLa cell lysates were analysed.First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Aprataxin antibody (ab154413) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

APTX knockout HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Saos-2 cell lysate at 20 µg

false

• WB

Unknown

Western blot - Anti-Aprataxin antibody (AB154413)

10% SDS PAGE

All lanes:

Western blot - Anti-Aprataxin antibody (ab154413) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 41 kDa,56 kDa

false

• WB

Lab

Western blot - Anti-Aprataxin antibody (AB154413)

False colour image of Western blot : Anti-Aprataxin antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab154413 was shown to bind specifically to Aprataxin. A band was observed at 41 kDa in wild-type HeLa cell lysates with no signal observed at this size in APTX CRISPR-Cas9 edited cell line ab265118 (CRISPR-Cas9 edited cell lysate ab257837). The band observed in the CRISPR-Cas9 edited lysate lane below 41 kDa is likely to represent a truncated form of Aprataxin. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and APTX CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Lanes 1, 2 and 4:

Western blot - Anti-Aprataxin antibody (ab154413) at 1/500 dilution

Lane 3:

Western blot - Anti-Aprataxin antibody (ab154413) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

APTX CRISPR-Cas9 edited HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human APTX (Aprataxin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-aptx-aprataxin-knockout-hela-cell-line-ab265118'>ab265118</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Saos-2 cell lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 41 kDa

false