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AB317382

Anti-Aprataxin Antibody [S03-9F6]

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Rabbit Monoclonal Aprataxin antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human APTX.

View Alternative Names

AXA1, APTX, Aprataxin, Forkhead-associated domain histidine triad-like protein, FHA-HIT

1 Images
Western blot - Anti-Aprataxin Antibody [S03-9F6] (AB317382)
  • WB

Supplier Data

Western blot - Anti-Aprataxin Antibody [S03-9F6] (AB317382)

All lanes:

Western blot - Anti-Aprataxin Antibody [S03-9F6] (ab317382) at 1/1000 dilution

Lane 1:

K562 cell lysate

Lane 2:

HeLa cell lysate

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S03-9F6

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human APTX.

Q7Z2E3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aprataxin also known as APTX is a protein involved in DNA repair. It belongs to the histidine triad superfamily and has a molecular mass of approximately 40 kDa. The protein is expressed in various tissues including the brain liver and kidney. Aprataxin's main role involves the repair of DNA strand breaks by removing abnormal DNA termini. It restores DNA integrity which is critical for maintaining cellular functions.
Biological function summary

Aprataxin plays an important role in the DNA single-strand break repair processes. It functions within the DNA repair machinery often as part of complexes with other repair proteins. This protein mainly interacts with other partners like XRCC1 and PARP1 to facilitate accurate DNA repair. Its interaction ensures proper response to DNA damage safeguarding the genome's stability and function.

Pathways

Aprataxin is integrally connected to the base excision repair (BER) pathway and the DNA damage response pathway. These pathways are fundamental mechanisms for repairing damaged DNA and preserving cell viability. Within these pathways Aprataxin closely collaborates with related proteins like XRCC1 and PARP1. These associations highlight Aprataxin's role in ensuring the efficiency and success of DNA repair processes.

Mutations or malfunctions in Aprataxin are linked to neurological conditions such as ataxia with oculomotor apraxia 1 (AOA1) and spinocerebellar ataxia with axonal neuropathy (SCAN1). These disorders are marked by progressive loss of movement coordination and peripheral neuropathy. In the context of these diseases Aprataxin interacts with proteins involved in neuronal maintenance and protection such as TDP-43 and p53. Disruptions in such interactions often result in the observed pathological manifestations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair (PubMed : 15044383, PubMed : 15380105, PubMed : 16964241, PubMed : 17276982, PubMed : 24362567). Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species (PubMed : 16964241, PubMed : 24362567). Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed : 16964241, PubMed : 17276982, PubMed : 24362567). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (PubMed : 16547001). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).
See full target information APTX
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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