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AB247609

Anti-APRIL/TNFSF13 antibody [EPR4286] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal APRIL/TNFSF13 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CD256, APRIL, TALL2, ZTNF2, UNQ383/PRO715, TNFSF13, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TALL-2, TRDL-1

1 Images
Western blot - Anti-APRIL/TNFSF13 antibody [EPR4286] - BSA and Azide free (AB247609)
  • WB

Unknown

Western blot - Anti-APRIL/TNFSF13 antibody [EPR4286] - BSA and Azide free (AB247609)

This data was developed using ab108206, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-APRIL/TNFSF13 antibody [EPR4286] (<a href='/en-us/products/primary-antibodies/april-tnfsf13-antibody-epr4286-ab108206'>ab108206</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

PC-12 cell lysate at 10 µg

Lane 5:

NIH 3T3 cell lysate at 10 µg

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4286

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247609 is the carrier-free version of ab108206.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APRIL also known as TNFSF13 is a member of the tumor necrosis factor (TNF) ligand superfamily. This protein has a mass of approximately 31 kDa and undergoes incorporation into different cellular processes. APRIL is mainly expressed in cells of the immune system such as T cells B cells and myeloid cells. The protein plays a critical role in modulating various biological activities including cell survival proliferation and apoptosis.
Biological function summary

APRIL functions as a cytokine involved in immune regulation. The molecule acts independently or as part of a ligand-receptor interaction binding specifically to receptors like BCMA and TACI. This interaction influences B-cell maturation and differentiation driving immune responses. APRIL helps maintain homeostasis within the immune system ensuring appropriate immune activation and preventing overactive immune responses.

Pathways

APRIL coordinates with several immune-related pathways notably the B-cell signaling pathway and the NF-kB signaling pathway. These pathways are essential in immune system modulation influencing how the body responds to pathogens. APRIL interacts with proteins such as BAFF (B-cell activating factor) which share common receptors shaping the immune response through these pathways and ensuring proper immune cell function.

APRIL has connections with conditions like autoimmune diseases and certain lymphomas. Elevated levels of APRIL often occur in diseases like lupus contributing to abnormal B-cell activity and autoantibody production. Additionally its involvement in lymphomas links to excessive proliferation of B cells. Proteins such as BAFF and their receptors play a significant role in these conditions highlighting APRIL's impact on disease mechanisms and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
See full target information TNFSF13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

TH open : companion journal to thrombosis and haemostasis 7:e217-e225 PubMed37501780

2023

Levels of Fibrinogen Variants Are Altered in Severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Judith J de Vries,Chantal Visser,Maureen van Ommen,Casper Rokx,Els van Nood,Eric C M van Gorp,Marco Goeijenbier,Johannes P C van den Akker,Henrik Endeman,Dingeman C Rijken,Marieke J H A Kruip,Miranda Weggeman,Jaap Koopman,Moniek P M de Maat
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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