AB196558

Anti-APRT antibody

Name: Anti-APRT antibody (ab196558) | Abcam
Brand: Abcam Limited
SKU: AB196558
Price: 545 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal APRT antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Adenine phosphoribosyltransferase.

View Alternative Names

Adenine phosphoribosyltransferase, APRT

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-APRT antibody (AB196558)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-APRT antibody (AB196558)

Immunofluorescence analysis of A549 cells labeling APRT using ab196558, at 1/50 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APRT antibody (AB196558)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APRT antibody (AB196558)

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling APRT with ab196558 at 1/200 dilution.

Supplier Data

Western blot - Anti-APRT antibody (AB196558)

All lanes:

Western blot - Anti-APRT antibody (ab196558) at 1/500 dilution

Lane 1:

MCF7 extract

Lane 2:

22RV-1 extract

Lane 3:

THP1 extract

Lane 4:

A549 extract

Lane 5:

K562 extract

Lane 6:

HeLa extract

Lane 7:

Mouse intestine extract

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Adenine phosphoribosyltransferase.

P07741

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenine phosphoribosyltransferase (APRT) also known as APTR is an enzyme involved in the purine salvage pathway. It has a molecular mass of approximately 23 kDa and gets expressed in numerous tissues including the liver kidney and brain. This protein catalyzes the conversion of adenine and phosphoribosyl pyrophosphate (PRPP) to adenosine monophosphate (AMP) facilitating the reutilization of adenine into nucleotides which is necessary for DNA and RNA synthesis.

Biological function summary

Adenine phosphoribosyltransferase plays a role in cellular nucleotide pool maintenance by rescuing adenine from degradation and incorporating it back into cellular metabolism. This enzyme does not form part of a larger enzyme complex but acts independently to perform its catalytic function. Properly maintaining nucleotide balance is fundamental for cellular activities and imbalances can lead to cellular stress or DNA damage responses.

Pathways

The purine salvage pathway is where adenine phosphoribosyltransferase functions critically. This pathway operates alongside the de novo synthesis of purine nucleotides working to conserve energy and resources during nucleotide synthesis. APRT interacts closely with hypoxanthine-guanine phosphoribosyltransferase (HGPRT) within this salvage process to recycle purines effectively reducing the need for energy-expensive de novo synthesis.

Defects in the adenine phosphoribosyltransferase gene can result in the condition known as APRT deficiency. This genetic disorder leads to the buildup of 28-dihydroxyadenine causing nephrolithiasis and potential kidney damage. APRT's function connects with xanthine oxidase activity influencing uric acid metabolism. The accumulation of poorly soluble adenine derivatives can also cause crystalluria and can influence uric acid levels connecting APRT to the pathophysiology of gout in some cases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

See full target information Adenine phosphoribosyltransferase

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1612390 PubMed40927188

2025

Identification and validation for biomarkers associated with mitochondrial metabolism in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Wang,Lanxiang Wu,Chao Ouyang,Tao Huang

Cell 187:3602-3618.e20 PubMed38823389

2024

De novo and salvage purine synthesis pathways across tissues and tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Diem H Tran,Dohun Kim,Rushendhiran Kesavan,Harrison Brown,Trishna Dey,Mona Hoseini Soflaee,Hieu S Vu,Alpaslan Tasdogan,Jason Guo,Divya Bezwada,Houssam Al Saad,Feng Cai,Ashley Solmonson,Halie Rion,Rawand Chabatya,Salma Merchant,Nathan J Manales,Vanina T Tcheuyap,Megan Mulkey,Thomas P Mathews,James Brugarolas,Sean J Morrison,Hao Zhu,Ralph J DeBerardinis,Gerta Hoxhaj

Nature communications 13:2698 PubMed35577785

2022

Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mona Hoseini Soflaee,Rushendhiran Kesavan,Umakant Sahu,Alpaslan Tasdogan,Elodie Villa,Zied Djabari,Feng Cai,Diem H Tran,Hieu S Vu,Eunus S Ali,Halie Rion,Brendan P O'Hara,Sherwin Kelekar,James Hughes Hallett,Misty Martin,Thomas P Mathews,Peng Gao,John M Asara,Brendan D Manning,Issam Ben-Sahra,Gerta Hoxhaj

Cell reports 39:110609 PubMed35385733

2022

Metabolism drives macrophage heterogeneity in the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Li,Jiali Yu,Amanda Huber,Ilona Kryczek,Zhuwen Wang,Long Jiang,Xiong Li,Wan Du,Gaopeng Li,Shuang Wei,Linda Vatan,Wojciech Szeliga,Arul M Chinnaiyan,Michael D Green,Marcin Cieslik,Weiping Zou

Nature metabolism 3:571-585 PubMed33833463

2021

Mitochondrial NADP is essential for proline biosynthesis during cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Diem H Tran,Rushendhiran Kesavan,Halie Rion,Mona Hoseini Soflaee,Ashley Solmonson,Divya Bezwada,Hieu S Vu,Feng Cai,John A Phillips,Ralph J DeBerardinis,Gerta Hoxhaj

Frontiers in cell and developmental biology 9:636802 PubMed33869182

2021

Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Chen,Mian Wu,Nan Liang,Junxi Lu,Shen Qu,Haibing Chen

Cell chemical biology 25:666-676.e4 PubMed29576532

2018

Structural Insights into the Forward and Reverse Enzymatic Reactions in Human Adenine Phosphoribosyltransferase.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Huyet,Mohammad Ozeir,Marie-Claude Burgevin,Benoît Pinson,Françoise Chesney,Jean-Marc Remy,Abdul Rauf Siddiqi,Roland Lupoli,Gregory Pinon,Christelle Saint-Marc,Jean-Francois Gibert,Renaud Morales,Irène Ceballos-Picot,Robert Barouki,Bertrand Daignan-Fornier,Anne Olivier-Bandini,Franck Augé,Pierre Nioche

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APRT antibody

Immunocytochemistry/ Immunofluorescence - Anti-APRT antibody (AB196558)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APRT antibody (AB196558)

Western blot - Anti-APRT antibody (AB196558)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise