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AB222414

Anti-APX2 antibody

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(3 Publications)

Rabbit Polyclonal APX2 antibody. Suitable for WB and reacts with Arabidopsis thaliana samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Arabidopsis thaliana APX2 aa 1-250.

View Alternative Names

APX1B, At3g09640, F11F8_23, APX2, L-ascorbate peroxidase 1b, APX1b, AtAPx02

1 Images
Western blot - Anti-APX2 antibody (AB222414)
  • WB

Supplier Data

Western blot - Anti-APX2 antibody (AB222414)

All lanes:

Western blot - Anti-APX2 antibody (ab222414) at 1/2000 dilution

Lane 1:

Arabidopsis thaliana lysate at 40 µg

Lane 2:

Arabidopsis thaliana lysate at 20 µg

Lane 3:

Arabidopsis thaliana lysate at 10 µg

Lane 4:

Arabidopsis thaliana lysate at 5 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Arabidopsis thaliana

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Arabidopsis thaliana APX2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q1PER6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Arabidopsis thaliana": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Precipitation Caprylic Acid and Ammonium Sulphate
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APX2 also known by its full name Ascorbate Peroxidase 2 plays a significant role in cellular mechanisms. This enzyme has a molecular mass of approximately 30 kDa and is predominantly found in chloroplasts in plant cells. APX2 catalyzes the conversion of hydrogen peroxide into water and oxygen using ascorbate as an electron donor. This action helps protect cells against oxidative damage by reducing reactive oxygen species levels.
Biological function summary

Enzymes like APX2 are vital components for maintaining cell health in plants. APX2 is a part of the antioxidant defense system which regulates reactive oxygen species and repairs damaged cellular components. It operates independently and does not appear to form part of a large protein complex. APX2 expression can change in response to environmental stressors such as high light intensity or drought showcasing its key role in plant stress responses.

Pathways

This protein plays an integral part in both the ascorbate-glutathione cycle and the oxidative stress response pathway which are important for detoxifying hydrogen peroxide in plants. Within these pathways it collaborates with enzymes like glutathione reductase and superoxide dismutase to coordinate the reduction of harmful oxygen species and support cellular resilience against stress.

The involvement of APX2 in managing oxidative stress may connect to several plant stress-related conditions such as leaf senescence and drought tolerance. The protein may interact with other stress-related proteins like catalase to mediate responses that prevent cellular damage in adverse conditions. Enhancing APX2 activity is considered a potential strategy in developing stress-resistant plants improving crop safety and yield under environmental pressure.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a key role in hydrogen peroxide removal.
See full target information APX2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bone research 13:60 PubMed40500265

2025

Inflammatory macrophage-derived itaconate inhibits DNA demethylase TET2 to prevent excessive osteoclast activation in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kewei Rong,Dezheng Wang,Xiting Pu,Cheng Zhang,Pu Zhang,Xiankun Cao,Jinglin Zheng,Xiao Yang,Kexin Liu,Lei Shi,Yin Li,Peixiang Ma,Dan Ye,Jie Zhao,Pu Wang,An Qin

Nature chemical biology 21:360-370 PubMed39223388

2024

An engineered trafficking biosensor reveals a role for DNAJC13 in DOR downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon Novy,Aleksandra Dagunts,Tatum Weishaar,Emily E Holland,Hayden Adoff,Emily Hutchinson,Monica De Maria,Martin Kampmann,Nikoleta G Tsvetanova,Braden T Lobingier

Nucleic acids research 48:6855-6873 PubMed32406909

2020

G3BP1-linked mRNA partitioning supports selective protein synthesis in response to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Syam Prakash Somasekharan,Fan Zhang,Neetu Saxena,Jia Ni Huang,I-Chih Kuo,Caitlin Low,Robert Bell,Hans Adomat,Nikolay Stoynov,Leonard Foster,Martin Gleave,Poul H Sorensen
View all publications
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