Rabbit Polyclonal AQP-6 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AQP6 aa 250 to C-terminus.
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Publications
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- JOR spine 3:e10982020Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbbie Binch et. al.PubMed 33015573
- Research in veterinary science 125:162-1692019Investigating cellular location of aquaporins in the bovine kidney. A new view on renal physiology in cattle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Michałek et. al.PubMed 31233960
- JOR spine 2:e10492019Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph W Snuggs et. al.PubMed 31463463
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human AQP6 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13520
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Target data
Function
Forms a water-specific channel that participates in distinct physiological functions such as glomerular filtration, tubular endocytosis and acid-base metabolism.
Alternative names
AQP2L, AQP6, Aquaporin-6, AQP-6, Aquaporin-2-like, Kidney-specific aquaporin, hKID
Recommended products
Rabbit Polyclonal AQP-6 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AQP6 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human AQP6 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13520
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aquaporin-6 (AQP-6) is an integral membrane protein and part of the aquaporin family primarily involved in water transport across cell membranes. It weighs approximately 28 kilodaltons. Unlike other aquaporins AQP-6 also allows passage of anions making it unique in its function within the family. AQP-6 is expressed mainly in kidney tissues particularly in renal collecting ducts where it facilitates fluid balance and ion exchange.
- Biological function summary
This protein plays a role in maintaining osmotic balance and modulating cell volume. AQP-6 interacts with other membrane proteins but it is not typically part of larger macromolecular complexes. Its activity contributes significantly to renal urine concentration influencing water reabsorption and ion transport in the kidneys. AQP-6 ion permeability distinguishes it from other aquaporins allowing it to assist in the rapid response to cellular pH changes and osmotic shifts.
- Pathways
AQP-6 is involved in the renal water reabsorption and acid-base balance pathways. It interacts with proteins such as AQP-1 and AQP-2 which are also prominent in water transport systems. AQP-6 affects physiological processes governed by hormonal regulation responding to vasopressin to modify water reabsorption rates accordingly.
- Associated diseases and disorders
Abnormalities in AQP-6 function or expression can relate to conditions like nephrogenic diabetes insipidus and acid-base disturbances. Dysfunctional AQP-6 impacts water balance and pH regulation leading to potential kidney-related disorders. The protein's interaction with AQP-2 in water reabsorption processes suggests a possible compensatory mechanism in these pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-AQP-6 antibody - C-terminal (ab191061)
All lanes: Western blot - Anti-AQP-6 antibody - C-terminal (ab191061) at 0.5 µg/mL
Lane 1: U87 cell lysate
Lane 2: COLO320 cell lysate
Lane 3: MCF7 cell lysate
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling AQP-6 with ab191061 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling AQP-6 with ab191061 at 1 μg/ml.
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