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AB134695

Anti-AQP0 antibody

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(4 Publications)

Rabbit Polyclonal AQP0 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MIP.

View Alternative Names

AQP0, MIP, Lens fiber major intrinsic protein, Aquaporin-0, MIP26, MP26

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP0 antibody (AB134695)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP0 antibody (AB134695)

Immunohistochemical analysis of AQP0 in paraffin-embedded Human brain tissue using ab134695 at 1/50 dilution.

Western blot - Anti-AQP0 antibody (AB134695)
  • WB

Unknown

Western blot - Anti-AQP0 antibody (AB134695)

All lanes:

Western blot - Anti-AQP0 antibody (ab134695) at 1/500 dilution

All lanes:

HT29 cell extract at 30 µg

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MIP. The exact immunogen used to generate this antibody is proprietary information.

P30301

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AQP0 also known as Aquaporin-0 or MIP (Major Intrinsic Protein) is a water channel protein with a molecular mass of approximately 28 kDa. This protein facilitates the transport of water molecules across cell membranes acting as a critical component in maintaining cellular water homeostasis. AQP0 is expressed primarily in the lens fiber cells of the eye where it plays a significant role in providing transparency and maintaining lens structure.
Biological function summary

AQP0 is essential for lens transparency and proper refraction facilitating the alignment and compaction of lens fiber cells. AQP0 acts as both a water channel and a cell-to-cell adhesion molecule in these cells. Through interactions with other proteins in the lens AQP0 contributes to maintaining the lens's unique architecture and mechanical stability although it typically does not form a larger complex on its own.

Pathways

AQP0 plays an important role in the regulation of water balance in the eye lens. The protein functions as an integral part of the water transport pathway in the lens and interacts with other aquaporins such as AQP1 and AQP5. Additionally AQP0 modulates lens permeability affecting pathways tied to lens osmolarity and transparency which are essential for normal vision function.

Mutations or misregulation of AQP0 are associated with cataracts a condition characterized by the clouding of the lens leading to impaired vision. Disruption in AQP0 functionality can alter lens transparency and homeostasis causing protein aggregation. Research has shown that AQP0 mutations can influence the interactions with other lens proteins like crystallins which are important for lens transparency ultimately leading to cataract development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (PubMed : 11001937, PubMed : 24120416). Specifically expressed in lens fiber cells, this aquaporin is crucial for maintaining lens water homeostasis and transparency. Beyond water permeability, it also acts as a cell-to-cell adhesion molecule, forming thin junctions between lens fiber cells that are essential for maintaining the ordered structure and transparency of the lens (PubMed : 24120416).
See full target information MIP
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

EJHaem 2:196-210 PubMed35845268

2021

Bimodal expression of during myelomonocytic differentiation: Implications for the expansion of AML differentiation therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvie Galiègue-Zouitina,Qiangwei Fu,Céline Carton-Latreche,Nicolas Poret,Meyling Cheok,Frédéric Leprêtre,Martin Figeac,Bruno Quesnel,Hassiba El Bouazzati,Carl S Shelley

JOR spine 3:e1098 PubMed33015573

2020

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie Binch,Joseph Snuggs,Christine L Le Maitre

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:2380-2391 PubMed32167668

2020

Signaling through tumor necrosis receptor 2 induces stem cell marker in CD133 regenerating tubular epithelial cells in acute cell-mediated rejection of human renal allografts.

Applications

Unspecified application

Species

Unspecified reactive species

John R Bradley,Jun Wang,Victoria Bardsley,Verena Broecker,Sathia Thiru,Jordan S Pober,Rafia S Al-Lamki

JOR spine 2:e1049 PubMed31463463

2019

Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph W Snuggs,Rebecca E Day,Frances C Bach,Matthew T Conner,Rowena A D Bunning,Marianna A Tryfonidou,Christine L Le Maitre
View all publications
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