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AB9566

Anti-Aquaporin 1 antibody [1/22]

4

(19 Reviews)

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(84 Publications)

Anti-Aquaporin 1 antibody [1/22] (ab9566) is a mouse monoclonal antibody detecting Aquaporin 1 in Western Blot, Flow Cytometry, IHC-P. Suitable for Human, Rat.

- Over 60 publications
- Trusted since 2002

View Alternative Names

CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (AB9566)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (AB9566)

Immunohistochemical analysis of exocrine pancreas cells labeling Aquaporin 1 with ab9566.

Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (AB9566)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (AB9566)

Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (AB9566)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (AB9566)

Immunohistochemical staining of aquaporin 1 in rat brain.

Western blot - Anti-Aquaporin 1 antibody [1/22] (AB9566)
  • WB

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Western blot - Anti-Aquaporin 1 antibody [1/22] (AB9566)

All lanes:

Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/mL

All lanes:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 28 kDa

Observed band size: 29 kDa,50 kDa,75 kDa

true

Exposure time: 30s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1/22

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human AQP1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P29972

Epitope

Intracellular C-terminal AQP 1 epitope (active channel loop)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Aquaporin 1 antibody [1/22] (ab9566) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Rat samples.

What is the molecular weight of Aquaporin 1?
Anti-Aquaporin 1 [1/22] (ab9566) specifically detects a band for Aquaporin 1 (UniProt: P29972) at a molecular weight of 29kDa.

Trusted by the scientific community
Anti-Aquaporin 1 [1/22] (ab9566) was first used in a scientific publication in 2002 and has been cited over 60 times in peer-reviewed journals.

Reviewed by scientists
Anti-Aquaporin 1 [1/22] (ab9566) has over 15 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.
Biological function summary

Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.

Pathways

The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.

Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed : 1373524, PubMed : 23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed : 16682607, PubMed : 17012249, PubMed : 19273840, PubMed : 33028705, PubMed : 8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed : 17012249, PubMed : 35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed : 36949749, PubMed : 8703053).
See full target information AQP1
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Publications (84)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadt1318 PubMed40668934

2025

Harnessing tissue-derived mitochondria-rich extracellular vesicles (Ti-mitoEVs) to boost mitochondrial biogenesis for regenerative medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Lou,Xiyue Zhou,Yimeng Zhang,Yijing Xie,Yizhuo Wang,Chengshi Wang,Shuyun Liu,Meihua Wan,Yanrong Lu,Jingping Liu

International journal of molecular sciences 26: PubMed40565279

2025

Molecular Determinants of the Human Retinal Pigment Epithelium Cell Fate and Potential Pharmacogenomic Targets for Precision Medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Zibetti

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 282:2911-2920 PubMed39725698

2024

Morphological studies of labyrinthine tissue in patients affected with Meniere's disease and vestibular schwannoma following labyrinthectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Ankit Ajmera,Nikeith John,Adrienne Morey,Nigel Biggs,Sean Flanagan,Peter Earls,Daniel Brown,Payal Mukherjee

Kidney diseases (Basel, Switzerland) 10:504-518 PubMed39664329

2024

Hypoxia Reduces Mouse Urine Output via HIF1α-Mediated Upregulation of Renal AQP1.

Applications

Unspecified application

Species

Unspecified reactive species

Rongfang Qiao,Xiaohui Cui,Yitong Hu,Haoqing Wei,Hu Xu,Cong Zhang,Chunxiu Du,Jiazhen Chang,Yaqing Li,Wenhua Ming,Yinghui Qi,Youfei Guan,Xiaoyan Zhang

Cell death discovery 10:448 PubMed39443466

2024

C/EBPα-mediated ACSL4-dependent ferroptosis exacerbates tubular injury in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ziru Xia,Zhaonan Wei,Xin Li,Yunzi Liu,Xiangchen Gu,Jianhua Tong,Siyi Huang,Xiaoyue Zhang,Weiming Wang

Communications biology 7:1278 PubMed39375428

2024

Generation of human-pig chimeric renal organoids using iPSC technology.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Fujimori,Shuichiro Yamanaka,Kentaro Shimada,Kenji Matsui,Shiho Kawagoe,Takao Kuroda,Atsushi Ikeda,Makoto Inoue,Eiji Kobayashi,Takashi Yokoo

Cell communication and signaling : CCS 22:357 PubMed38987851

2024

Macrophage-derived exosomes promote telomere fragility and senescence in tubular epithelial cells by delivering miR-155.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Yin,Tao-Tao Tang,Xiao-Yu Lu,Wei-Jie Ni,Di Yin,Yi-Lin Zhang,Wei Jiang,Yue Zhang,Zuo-Lin Li,Yi Wen,Wei-Hua Gan,Ai-Qing Zhang,Lin-Li Lv,Bin Wang,Bi-Cheng Liu

Cell reports 43:114395 PubMed38941187

2024

Macrophages preserve endothelial cell specialization in the adrenal gland to modulate aldosterone secretion and blood pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Fan,Mara Karakone,Shunmugam Nagarajan,Nadine Nagy,Wiebke Mildenberger,Ekaterina Petrova,Laura Catharina Hinte,Mitchell Bijnen,Philipp Häne,Eric Nelius,Jing Chen,Irina Ferapontova,Ferdinand von Meyenn,Francesco Trepiccione,Mesut Berber,David Penton Ribas,Anne Eichmann,Maria-Christina Zennaro,Norihiko Takeda,Jens W Fischer,Ariadni Spyroglou,Martin Reincke,Felix Beuschlein,Johannes Loffing,Melanie Greter,Christian Stockmann

Nanoscale advances 6:276-286 PubMed38125591

2023

Lithographically defined encoded magnetic heterostructures for the targeted screening of kidney cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Selma Leulmi Pichot,Tarun Vemulkar,Jeroen Verheyen,Lauren Wallis,James O Jones,Andrew P Stewart,Sarah J Welsh,Grant D Stewart,Russell P Cowburn

Acta pharmacologica Sinica 45:125-136 PubMed37684381

2023

PGAM5 exacerbates acute renal injury by initiating mitochondria-dependent apoptosis by facilitating mitochondrial cytochrome c release.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Yao Li,Xi-Ang Sun,Xin Wang,Ning-Hao Yang,Hong-Yan Xie,Heng-Jiang Guo,Li Lu,Xin Xie,Li Zhou,Jun Liu,Wei Zhang,Li-Min Lu
View all publications
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