Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387 is a rabbit monoclonal antibody that is used in Aquaporin 1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR11588(B) is cited in over 50 publications
- One antibody for all your Aquaporin 1 staining
Images
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--western-blot-img167968.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)")
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--western-blot-img166643.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175701.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)")
![Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunocytochemistry-immunofluorescence-img170724.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)")
![Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--flow-cytometry-intracellular-img164745.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)")
Publications
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- BMC neuroscience 24:602023Cycle-dependent sex differences in expression of membrane proteins involved in cerebrospinal fluid secretion at rat choroid plexus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIda Marchen Egerod Israelsen,Christina Kamp-Jensen,Connar Stanley James Westgate,Bjarne Styrishave,Rigmor H Jensen,Sajedeh EftekhariPubMed 37946101
- iScience 26:1082112023Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan Guo,Daoqi Shen,Yiqi Su,Jiarui Xu,Shuan Zhao,Weidong Zhang,Yaqiong Wang,Wuhua Jiang,Jialin Wang,Xuemei Geng,Xiaoqiang Ding,Xialian XuPubMed 37942007
- Cell death & disease 14:6632023Pyruvate kinase M2 regulates mitochondrial homeostasis in cisplatin-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjia Xie,Qingyun He,Yan Zhang,Xinxin Xu,Ping Wen,Hongdi Cao,Yang Zhou,Jing Luo,Junwei Yang,Lei JiangPubMed 37816709
- PloS one 18:e02885072023Endotoxin induced acute kidney injury modulates expression of AQP1, P53 and P21 in rat kidney, heart, lung and small intestine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWuyang Lv,Longge Xue,Lei Liang,Dongyang Liu,Cuicui Li,Jia Liao,Yingyu JinPubMed 37490500
- Pathology oncology research : POR 29:16111792023Expression of aquaporin 1, 3 and 5 in colorectal carcinoma: correlation with clinicopathological characteristics and prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangwen Zhang,Yongfei Hao,Ling Chen,Zengshan Li,Langlang Gao,Jian Tian,Qing Qiao,Jinsong ZhangPubMed 37334171
- Frontiers in endocrinology 14:10310662023Modulation of vulvovaginal atrophy (VVA) by Gelam honey in bilateral oophorectomized rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNur Hilwani Ismail,Siti Fatimah Ibrahim,Mohd Helmy Mokhtar,Azyani Yahaya,Aini Farzana Zulkefli,Sheril June Ankasha,Khairul OsmanPubMed 36923220
- Scientific reports 12:185702022Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyuki Nakamura,Tsutomu Tanaka,Changyu Zheng,Sandra A Afione,Blake M Warner,Masayuki Noguchi,Tatsuya Atsumi,John A ChioriniPubMed 36329045
- JCI insight 7:2022Integrated single-cell transcriptomics and proteomics reveal cellular-specific responses and microenvironment remodeling in aristolochic acid nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayun Chen,Piao Luo,Chen Wang,Chuanbin Yang,Yunmeng Bai,Xueling He,Qian Zhang,Junzhe Zhang,Jing Yang,Shuang Wang,Jigang WangPubMed 35852860
- Scientific reports 12:91022022The impact of obesity-related raised intracranial pressure in rodents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesConnar Stanley James Westgate,Snorre Malm Hagen,Ida Marchen Egerod Israelsen,Steffen Hamann,Rigmor Højland Jensen,Sajedeh EftekhariPubMed 35650312
- Scientific reports 12:62632022Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHideto Deguchi,Tomoko Yamashita,Nao Hiramoto,Yohei Otsuki,Atsushi Mukai,Morio Ueno,Chie Sotozono,Shigeru Kinoshita,Junji HamuroPubMed 35428816
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Expected |
| Mouse | Expected | Expected | Not recommended | Expected | Tested |
| Rat | Expected | Expected | Not recommended | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/8000 | Notes For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes For unpurified use at 1/1000 - 1/10000. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Species Rat | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed:1373524, PubMed:23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed:16682607, PubMed:17012249, PubMed:19273840, PubMed:33028705, PubMed:8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed:17012249, PubMed:35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed:36949749, PubMed:8703053).
Alternative names
CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel
Recommended products
Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387 is a rabbit monoclonal antibody that is used in Aquaporin 1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR11588(B) is cited in over 50 publications
- One antibody for all your Aquaporin 1 staining
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11588(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.
- Biological function summary
Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.
- Pathways
The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.
- Associated diseases and disorders
Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--western-blot-img167968.jpg "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Unglycosylated AQP-1: 28kDa; Glycosylated AQP-1: 35-50kDa
All lanes: Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution
Lane 1: Human heart lysate unboiled
Lane 2: Human spleen lysate unboiled
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDa
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--western-blot-img166643.jpg "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Unglycosylated AQP-1: 28kDa; Glycosylated AQP-1: 35-50kDa
All lanes: Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution
Lane 1: Mouse kidney lysate boiled at 20 µg
Lane 2: Mouse kidney lysate unboiled at 20 µg
Lane 3: Rat kidney lysate boiled at 20 µg
Lane 4: Rat kidney lysate unboiled at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175701.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunocytochemistry-immunofluorescence-img170724.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labeling Aquaporin 1 with purified ab168387 at 1/50 dilution (3.9 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with N/A. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--flow-cytometry-intracellular-img164745.jpg "Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Flow Cytometry (Intracellular) analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labelling Aquaporin 1 with purified ab168387 at 1/2000 dilution (0.1 μg/mL, Right) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabelled control - /.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166566.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170473.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166566.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-ab168387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170473.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
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