Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)

This data was developed using ab168387, the same antibody clone in a different buffer formulation.
Flow Cytometry (Intracellular) analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labelling Aquaporin 1 with purified ab168387 at 1/2000 dilution (0.1 μg/mL, Right) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabelled control - /.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)

This data was developed using ab168387, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labeling Aquaporin 1 with purified ab168387 at 1/50 dilution (3.9 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with N/A. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)

This data was developed using ab168387, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)

This data was developed using ab168387, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)

This data was developed using ab168387, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Supplier Data

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)

Unglycosylated AQP-1 : 28kDa; Glycosylated AQP-1 : 35-50kDa.

This data was developed using ab168387, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (<a href='/en-us/products/primary-antibodies/aquaporin-1-antibody-epr11588b-ab168387'>ab168387</a>) at 1/10000 dilution

Lane 1:

Human heart lysate unboiled at 20 µg

Lane 2:

Human spleen lysate unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 28 kDa

false

• WB

Unknown

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)

Unglycosylated AQP-1 : 28kDa; Glycosylated AQP-1 : 35-50kDa. This data was developed using ab168387, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (<a href='/en-us/products/primary-antibodies/aquaporin-1-antibody-epr11588b-ab168387'>ab168387</a>) at 1/10000 dilution

Lane 1:

Mouse kidney lysate boiled at 20 µg

Lane 2:

Mouse kidney lysate unboiled at 20 µg

Lane 3:

Rat kidney lysate boiled at 20 µg

Lane 4:

Rat kidney lysate unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 28 kDa

false