Rabbit Recombinant Monoclonal Aquaporin 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--western-blot-img167968.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)")
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--western-blot-img166643.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175701.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)")
![Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunocytochemistry-immunofluorescence-img170724.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)")
![Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--flow-cytometry-intracellular-img164745.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (AB178352)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Tested | Expected |
| Mouse | Expected | Tested | Not recommended | Tested | Tested |
| Rat | Expected | Tested | Not recommended | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
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Target data
Function
Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed:1373524, PubMed:23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed:16682607, PubMed:17012249, PubMed:19273840, PubMed:33028705, PubMed:8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed:17012249, PubMed:35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed:36949749, PubMed:8703053).
Alternative names
CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel
Recommended products
Rabbit Recombinant Monoclonal Aquaporin 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11588(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab178352 is the carrier-free version of Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.
- Biological function summary
Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.
- Pathways
The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.
- Associated diseases and disorders
Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--western-blot-img167968.jpg "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
Unglycosylated AQP-1: 28kDa; Glycosylated AQP-1: 35-50kDa.
This data was developed using Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387) at 1/10000 dilution
Lane 1: Human heart lysate unboiled
Lane 2: Human spleen lysate unboiled
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDa
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--western-blot-img166643.jpg "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
Unglycosylated AQP-1: 28kDa; Glycosylated AQP-1: 35-50kDa.
This data was developed using Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387, the same antibody clone in a different buffer formulation.All lanes: Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387) at 1/10000 dilution
Lane 1: Mouse kidney lysate boiled at 20 µg
Lane 2: Mouse kidney lysate unboiled at 20 µg
Lane 3: Rat kidney lysate boiled at 20 µg
Lane 4: Rat kidney lysate unboiled at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175701.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
This data was developed using Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aquaporin 1 with purified Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunocytochemistry-immunofluorescence-img170724.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
This data was developed using Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labeling Aquaporin 1 with purified Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387 at 1/50 dilution (3.9 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with N/A. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--flow-cytometry-intracellular-img164745.jpg "Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
This data was developed using Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387, the same antibody clone in a different buffer formulation.
Flow Cytometry (Intracellular) analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labelling Aquaporin 1 with purified Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387 at 1/2000 dilution (0.1 μg/mL, Right) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabelled control - /.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166566.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
This data was developed using Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aquaporin 1 with purified Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170473.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
This data was developed using Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aquaporin 1 with purified Anti-Aquaporin 1 antibody [EPR11588(B)] ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166566.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-1-antibody-epr11588b-bsa-and-azide-free-ab178352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170473.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)")
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