AB219055

Anti-Aquaporin 1 antibody [EPR20325]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal Aquaporin 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel

8 Images

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Aquaporin 1 construct cellslabeling Aquaporin 1 with ab219055 at 1/700 dilution (right). Arabbit monoclonal IgG (ab172730) was used as isotype control (left). Goat anti rabbit IgG (Alexa Fluor^® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

Quadrant markers were set based on control antibody staining (ab172730) and GFP expression. Only GFP positive population (Q2 of right panel) give Aquaporin 1positive staining.

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged Aquaporin 1 expression construct or a GFP only control, labeling Aquaporin 1 with ab219055 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 647) (ab150079) secondary antibody at 1/1000 dilution.

Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 647) (ab150079) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on proximal tubules of human kidney is observed [PMID : 14592814].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on centroacinar cells of human pancreas is observed [PMID : 12801959].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on vessels of human cardiac muscle is observed [PMID : 14592814].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Aquaporin 1 was immunoprecipitated from 0.35 mg of whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Auaporin 1 construct, with ab219055 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab219055 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct, 10 μg (Input).

Lane 2 : ab219055 IP in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219055 in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Predicted band size: 28 kDa

Observed band size: 48 kDa

false

Supplier Data

Western blot - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Aquaporin 1 antibody [EPR20325] (ab219055) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Aquaporin 1 antibody [EPR20325] (ab219055) at 1/1000 dilution

Lane 1:

Whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with an empty expression vector at 10 µg

Lane 2:

Whole cell lysate from HEK-293T transfected with a GFP-tagged human Aquaporin 1 construct at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 28 kDa

Observed band size: 48 kDa

false

Exposure time: 2s

Carrier free

Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free
578 PE

PE Anti-Aquaporin 1 antibody [EPR20325]
660 APC

APC Anti-Aquaporin 1 antibody [EPR20325]
HRP

HRP Anti-Aquaporin 1 antibody [EPR20325]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Aquaporin 1 antibody [EPR20325]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Aquaporin 1 antibody [EPR20325]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Aquaporin 1 antibody [EPR20325]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Aquaporin 1 antibody [EPR20325]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Aquaporin 1 antibody [EPR20325]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20325

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/700", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.

Biological function summary

Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.

Pathways

The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.

Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed : 1373524, PubMed : 23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed : 16682607, PubMed : 17012249, PubMed : 19273840, PubMed : 33028705, PubMed : 8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed : 17012249, PubMed : 35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed : 36949749, PubMed : 8703053).

See full target information AQP1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:3821-3836 PubMed40213659

2025

Investigating the relationships of structural and functional neural networks of primary visual cortex with engineered AAVs and chemogenetic-fMRI techniques.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Gui,Kunzhang Lin,Nengsong Luo,Zengpeng Han,Zhuang Liu,Ziyue Zhao,Lei Gu,Fanyong Xu,Ning Zheng,Fuqiang Xu,Xihai Li,Jie Wang

Bioengineering (Basel, Switzerland) 11: PubMed38247931

2024

Electrospun Nanofiber Membrane for Cultured Corneal Endothelial Cell Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Euisun Song,Karen M Chen,Mathew S Margolis,Thitima Wungcharoen,Won-Gun Koh,David Myung

Cellular and molecular biology (Noisy-le-Grand, France) 69:227-232 PubMed38015518

2023

Actin cytoskeleton aggregation involves the water channel protein aquaporin 1-mediated human chondrocyte degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Chen,Xiucai Ma,Peng Liu,Xiao Yang,Yajun Yang

NeuroImage 258:119402 PubMed35732245

2022

A novel technology for in vivo detection of cell type-specific neural connection with AQP1-encoding rAAV2-retro vector and metal-free MRI.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Zheng,Mei Li,Yang Wu,Challika Kaewborisuth,Zhen Li,Zhu Gui,Jinfeng Wu,Aoling Cai,Kangguang Lin,Kuan-Pin Su,Hongbing Xiang,Xuebi Tian,Anne Manyande,Fuqiang Xu,Jie Wang

Cancers 13: PubMed34066403

2021

Anti-Angiogenic Properties of Ginsenoside Rg3 Epimers: In Vitro Assessment of Single and Combination Treatments.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Nakhjavani,Eric Smith,Kenny Yeo,Helen M Palethorpe,Yoko Tomita,Tim J Price,Amanda R Townsend,Jennifer E Hardingham

EBioMedicine 41:200-213 PubMed30796006

2019

LncRNA EPB41L4A-AS1 regulates glycolysis and glutaminolysis by mediating nucleolar translocation of HDAC2.

Applications

Unspecified application

Species

Unspecified reactive species

Meijian Liao,Weijie Liao,Naihan Xu,Bing Li,Fuhai Liu,Shikuan Zhang,Yanzhi Wang,Songmao Wang,Yuanchang Zhu,Deheng Chen,Weidong Xie,Yuyang Jiang,Liu Cao,Burton B Yang,Yaou Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aquaporin 1 antibody [EPR20325]

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Western blot - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

Western blot - Anti-Aquaporin 1 antibody [EPR20325] (AB219055)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise