AB232399

Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal Aquaporin 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged Aquaporin 1 expression construct or a GFP only control, labeling Aquaporin 1 with ab219055 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 647) (ab150079) secondary antibody at 1/1000 dilution.

Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 647) (ab150079) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on proximal tubules of human kidney is observed [PMID : 14592814].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on centroacinar cells of human pancreas is observed [PMID : 12801959]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on vessels of human cardiac muscle is observed [PMID : 14592814].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Aquaporin 1 construct cells labeling Aquaporin 1 with ab219055 at 1/700 dilution (right). A rabbit monoclonal IgG (ab172730) was used as isotype control (left). Goat anti rabbit IgG (Alexa Fluor^® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

Quadrant markers were set based on control antibody staining (ab172730) and GFP expression. Only GFP positive population (Q2 of right panel) give Aquaporin 1 positive staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

Supplier Data

Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Aquaporin 1 was immunoprecipitated from 0.35 mg of whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Auaporin 1 construct, with ab219055 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab219055 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct, 10 μg (Input).

Lane 2 : ab219055 IP in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219055 in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

All lanes:

Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] (<a href='/en-us/products/primary-antibodies/aquaporin-1-antibody-epr20325-ab219055'>ab219055</a>)

Predicted band size: 28 kDa

Observed band size: 48 kDa

false

Unconjugated

Anti-Aquaporin 1 antibody [EPR20325]
578 PE

PE Anti-Aquaporin 1 antibody [EPR20325]
660 APC

APC Anti-Aquaporin 1 antibody [EPR20325]
HRP

HRP Anti-Aquaporin 1 antibody [EPR20325]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Aquaporin 1 antibody [EPR20325]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Aquaporin 1 antibody [EPR20325]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Aquaporin 1 antibody [EPR20325]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Aquaporin 1 antibody [EPR20325]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Aquaporin 1 antibody [EPR20325]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20325

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab232399 is the carrier-free version of ab219055.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.

Biological function summary

Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.

Pathways

The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.

Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed : 1373524, PubMed : 23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed : 16682607, PubMed : 17012249, PubMed : 19273840, PubMed : 33028705, PubMed : 8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed : 17012249, PubMed : 35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed : 36949749, PubMed : 8703053).

See full target information AQP1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer chemotherapy and pharmacology 92:71-81 PubMed37272931

2023

Tumor-associated macrophages promote cisplatin resistance in ovarian cancer cells by enhancing WTAP-mediated N6-methyladenosine RNA methylation via the CXCL16/CXCR6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Hong,Xiuzhen Wang,Lang Zheng,Shengtan Wang,Genhai Zhu

Molecular & cellular proteomics : MCP 22:100526 PubMed36889440

2023

Integrated MicroRNA and Secretome Analysis of Human Endometrial Organoids Reveal the miR-3194-5p/Aquaporin/S100A9 Module in Regulating Trophoblast Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Dong,Jianlin Li,Dandan Cao,Jiangming Zhong,Xiaofeng Liu,Yong-Gang Duan,Kai-Fai Lee,William S B Yeung,Cheuk-Lun Lee,Philip C N Chiu

Cell death & disease 11:544 PubMed32681069

2020

Effects of ZnT8 on epithelial-to-mesenchymal transition and tubulointerstitial fibrosis in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuli Zhang,Tingwen Guan,Boxuan Yang,Harvest F Gu,Zhihong Chi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise