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Rabbit Recombinant Monoclonal Aquaporin 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399), expandable thumbnail
  • Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (AB232399), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Expected
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

8 products for Alternative Product

8 products for Alternative Version

Target data

Function

Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed:1373524, PubMed:23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed:16682607, PubMed:17012249, PubMed:19273840, PubMed:33028705, PubMed:8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed:17012249, PubMed:35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed:36949749, PubMed:8703053).

Alternative names

CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel

Recommended products

Rabbit Recombinant Monoclonal Aquaporin 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR20325
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab232399 is the carrier-free version of Anti-Aquaporin 1 antibody [EPR20325] ab219055.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.

Biological function summary

Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.

Pathways

The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.

Associated diseases and disorders

Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aquaporin 1 with Anti-Aquaporin 1 antibody [EPR20325] ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on centroacinar cells of human pancreas is observed [PMID: 12801959]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Aquaporin 1 antibody [EPR20325] ab219055).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged Aquaporin 1 expression construct or a GFP only control, labeling Aquaporin 1 with Anti-Aquaporin 1 antibody [EPR20325] ab219055 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) secondary antibody at 1/1000 dilution.

    Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Aquaporin 1 antibody [EPR20325] ab219055).

  • Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399), expandable thumbnail

    Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Aquaporin 1 was immunoprecipitated from 0.35 mg of whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Auaporin 1 construct, with Anti-Aquaporin 1 antibody [EPR20325] ab219055 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using Anti-Aquaporin 1 antibody [EPR20325] ab219055 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct, 10 μg (Input).

    Lane 2: Anti-Aquaporin 1 antibody [EPR20325] ab219055 IP in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Aquaporin 1 antibody [EPR20325] ab219055 in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Aquaporin 1 antibody [EPR20325] ab219055).

    All lanes: Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] (Anti-Aquaporin 1 antibody [EPR20325] ab219055)

    Predicted band size: 28 kDa

    Observed band size: 48 kDa

  • Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Aquaporin 1 construct cells labeling Aquaporin 1 with Anti-Aquaporin 1 antibody [EPR20325] ab219055 at 1/700 dilution (right). A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as isotype control (left). Goat anti rabbit IgG (Alexa Fluor® 647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody.

    Quadrant markers were set based on control antibody staining (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) and GFP expression. Only GFP positive population (Q2 of right panel) give Aquaporin 1 positive staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Aquaporin 1 antibody [EPR20325] ab219055).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 1 with Anti-Aquaporin 1 antibody [EPR20325] ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on proximal tubules of human kidney is observed [PMID: 14592814].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Aquaporin 1 antibody [EPR20325] ab219055).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Aquaporin 1 with Anti-Aquaporin 1 antibody [EPR20325] ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
    Membranous staining on vessels of human cardiac muscle is observed [PMID: 14592814].
    Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Aquaporin 1 antibody [EPR20325] ab219055).
    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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