Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)

Rabbit Monoclonal Aquaporin 1 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	ICC/IF	IP	Flow Cyt
Human	Tested	Tested	Expected	Not recommended	Expected	Not recommended
Mouse	Tested	Tested	Tested	Not recommended	Tested	Not recommended
Rat	Tested	Tested	Tested	Not recommended	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Target data

See full target information AQP1

Function

Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed:1373524, PubMed:23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed:16682607, PubMed:17012249, PubMed:19273840, PubMed:33028705, PubMed:8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed:17012249, PubMed:35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed:36949749, PubMed:8703053).

Alternative names

CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel

Recommended products

Rabbit Monoclonal Aquaporin 1 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR26554-46
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.

Biological function summary

Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.

Pathways

The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.

Associated diseases and disorders

Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling Aquaporin-1 with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/500 (0.912 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling Aquaporin-1 with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/500 (0.912 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
Aquaporin-1 was immunoprecipitated from 0.35 mg Mouse kidney tissue lysate 10 ug with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

Lane 1: Mouse kidney tissue lysate 10 µg
Lane 2: Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 IP in Mouse kidney tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 in Mouse kidney tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5.5 seconds
All lanes: Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR26554-46] (Anti-Aquaporin 1 antibody [EPR26554-46] ab300463) at 1/1000 dilution
All lanes: Mouse kidney tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 5.5s
Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (Anti-Aquaporin 1 antibody [EPR26554-46] ab300463) at 1/1000 dilution
Lane 1: Rat kidney tissue lysate
Lane 2: Rat brain tissue lysate
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution
Performed under reducing conditions.
Observed band size: 28 kDa, 35 kDa
Exposure time: 3.25s
Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (Anti-Aquaporin 1 antibody [EPR26554-46] ab300463) at 1/1000 dilution
Lane 1: Human kidney tissue lysate
Lane 2: Human lu tissue lysate 20
Lane 3: Mouse kidney tissue lysate
Lane 4: Mouse lu tissue lysate 20
Lane 5: Mouse brain tissue lysate
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution
Performed under reducing conditions.
Observed band size: 28 kDa, 35 kDa
Exposure time: 7.75s
Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (Anti-Aquaporin 1 antibody [EPR26554-46] ab300463) at 1/1000 dilution
Lane 1: Untreated Mouse kidney tissue lysate
Lane 2: Mouse kidney tissue lysate treated with Protein Deglycosylation MIX II
Lane 3: Untreated Rat kidney tissue lysate
Lane 4: Rat kidney tissue lysate treated with Protein Deglycosylation MIX II
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Performed under reducing conditions.
Observed band size: 28 kDa, 35 kDa
Exposure time: 1s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Aquaporin-1 with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/5000 (0.091 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on rat kidney (PMID: 11316845).The section was incubated with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Aquaporin-1 with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/5000 (0.091 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse kidney (PMID: 21712517).The section was incubated with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Aquaporin-1 with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/5000 (0.091 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Membraneous staining on human pancreas (PMID: 12801959).The section was incubated with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (ab300464)
This data was developed using Anti-Aquaporin 1 antibody [EPR26554-46] ab300463, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Aquaporin-1 with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 at 1/5000 (0.091 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human kidney (PMID: 14514735).The section was incubated with Anti-Aquaporin 1 antibody [EPR26554-46] ab300463 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins