AB233742

Anti-Aquaporin 2 antibody [7H8B5]

Name: Anti-Aquaporin 2 antibody [7H8B5] (ab233742) | Abcam
Brand: Abcam Limited
SKU: AB233742

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(1 Publication)

Mouse Monoclonal Aquaporin 2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AQP2 aa 100 to C-terminus.

View Alternative Names

Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, AQP-CD, AQP2

3 Images

Supplier Data

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (AB233742)

All lanes:

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (ab233742) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

HCT116 (human colorectal carcinoma cell line) cell lysate

Lane 4:

SW480 (Human colorectal adenocarcinoma cell line) cell lysate

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (AB233742)

All lanes:

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (ab233742) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Aquaporine 2-hIgGFc transfected HEK-293 cell lysate.

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (AB233742)

All lanes:

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (ab233742) at 1/500 dilution

All lanes:

Recombinant human Aquaporin 2 protein

Predicted band size: 28 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7H8B5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human AQP2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P41181

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 2 often referred to as AQP2 or aquaporin-2 channels is a water channel protein facilitating water transport across cell membranes. This protein belongs to the larger family of aquaporins which includes variants such as aquaporine often referred to interchangeably. Aquaporin 2 is a glycosylated integral membrane protein with an estimated mass of about 29 kDa. It expresses mainly in the kidney specifically in the collecting duct cells where it plays a pivotal role in water reabsorption.

Biological function summary

Aquaporin 2 serves a critical function in maintaining body water balance homeostasis. AQP2 responds to vasopressin a hormone that triggers its translocation to the apical membrane of collecting duct cells enabling water permeability. It is not part of a broader protein complex but operates independently within the cell membrane. This activity regulates urine concentration contributing to the maintenance of body fluids under variable hydration conditions.

Pathways

Aquaporin 2 contributes prominently to the vasopressin-regulated water reabsorption pathway and relates to the renal water reabsorption pathway. These pathways are important for interpreting how water homeostasis is managed by the body. Proteins such as the vasopressin receptor (also known as AVPR2) interact directly with AQP2 enabling its regulation and function. The interaction with cAMP signaling pathways facilitates the relocation of aquaporin 2 to its active site on the cell membrane ensuring efficient water reabsorption.

Mutations or dysregulation in aquaporin 2 can cause conditions like nephrogenic diabetes insipidus characterized by an inability to concentrate urine leading to excessive urination and thirst. Another disorder associated with AQP2 is syndrome of inappropriate antidiuretic hormone secretion (SIADH). These conditions highlight the importance of aquaporin 2 in fluid homeostasis and they connect it to regulatory proteins such as vasopressin itself emphasizing the interconnected nature of cellular proteins in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed : 15509592, PubMed : 7510718, PubMed : 7524315, PubMed : 8140421, PubMed : 8584435). Plays an essential role in renal water homeostasis (PubMed : 15509592, PubMed : 7524315, PubMed : 8140421). Could also be permeable to glycerol (PubMed : 8584435).

See full target information AQP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:34 PubMed40260066

2025

A disintegrin-like and metalloproteinase 15 facilitates glioblastoma proliferation and metastasis through activation of the protease-activated receptor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Ren,Zuowei Li,Qiong Fang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Aquaporin 2 antibody [7H8B5]

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (AB233742)

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (AB233742)

Western blot - Anti-Aquaporin 2 antibody [7H8B5] (AB233742)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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