AB62628

Anti-Aquaporin 2 antibody

Name: Anti-Aquaporin 2 antibody (ab62628) | Abcam
Brand: Abcam Limited
SKU: AB62628
Price: 515 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(12 Publications)

Anti-Aquaporin 2 antibody (ab62628) is a rabbit polyclonal antibody detecting Aquaporin 2 in ICC/IF. Suitable for Human.

- Trusted since 2008

View Alternative Names

Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, AQP-CD, AQP2

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 2 antibody (AB62628)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 2 antibody (AB62628)

Immunofluorescence analysis of HeLa cells, using 1/500-1/1000 ab62628.

Left image untreated.

Right image treated with peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human AQP2. The exact immunogen used to generate this antibody is proprietary information.

P41181

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Aquaporin 2 antibody (ab62628) is a rabbit polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 2 often referred to as AQP2 or aquaporin-2 channels is a water channel protein facilitating water transport across cell membranes. This protein belongs to the larger family of aquaporins which includes variants such as aquaporine often referred to interchangeably. Aquaporin 2 is a glycosylated integral membrane protein with an estimated mass of about 29 kDa. It expresses mainly in the kidney specifically in the collecting duct cells where it plays a pivotal role in water reabsorption.

Biological function summary

Aquaporin 2 serves a critical function in maintaining body water balance homeostasis. AQP2 responds to vasopressin a hormone that triggers its translocation to the apical membrane of collecting duct cells enabling water permeability. It is not part of a broader protein complex but operates independently within the cell membrane. This activity regulates urine concentration contributing to the maintenance of body fluids under variable hydration conditions.

Pathways

Aquaporin 2 contributes prominently to the vasopressin-regulated water reabsorption pathway and relates to the renal water reabsorption pathway. These pathways are important for interpreting how water homeostasis is managed by the body. Proteins such as the vasopressin receptor (also known as AVPR2) interact directly with AQP2 enabling its regulation and function. The interaction with cAMP signaling pathways facilitates the relocation of aquaporin 2 to its active site on the cell membrane ensuring efficient water reabsorption.

Mutations or dysregulation in aquaporin 2 can cause conditions like nephrogenic diabetes insipidus characterized by an inability to concentrate urine leading to excessive urination and thirst. Another disorder associated with AQP2 is syndrome of inappropriate antidiuretic hormone secretion (SIADH). These conditions highlight the importance of aquaporin 2 in fluid homeostasis and they connect it to regulatory proteins such as vasopressin itself emphasizing the interconnected nature of cellular proteins in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed : 15509592, PubMed : 7510718, PubMed : 7524315, PubMed : 8140421, PubMed : 8584435). Plays an essential role in renal water homeostasis (PubMed : 15509592, PubMed : 7524315, PubMed : 8140421). Could also be permeable to glycerol (PubMed : 8584435).

See full target information AQP2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:11486 PubMed40181096

2025

Glucagon like peptide-1 modulates urinary sodium excretion in diabetic kidney disease via ENaC activation.

Applications

Unspecified application

Species

Unspecified reactive species

Goh Kodama,Kensei Taguchi,Sakuya Ito,Yuri Nishino,Kayo Mori,Satomi Taguchi,Yuta Mitsuishi,Yuya Yamashita,Sho-Ichi Yamagishi,Kei Fukami

Scientific reports 14:15140 PubMed38956234

2024

Rapamycin-encapsulated nanoparticle delivery in polycystic kidney disease mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shinobu Yamaguchi,Randee Sedaka,Chintan Kapadia,Jifeng Huang,Jung-Shan Hsu,Taylor F Berryhill,Landon Wilson,Stephen Barnes,Caleb Lovelady,Yasin Oduk,Ryan M Williams,Edgar A Jaimes,Daniel A Heller,Takamitsu Saigusa

Frontiers in cell and developmental biology 11:1171637 PubMed37215091

2023

Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Shiting Liang,Youliang Wang,Meixia Kang,Juan Deng,Liting Chen,Xizhen Hong,Fan Fan Hou,Fujian Zhang

Nature communications 13:5805 PubMed36195583

2022

Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Letizia De Chiara,Carolina Conte,Roberto Semeraro,Paula Diaz-Bulnes,Maria Lucia Angelotti,Benedetta Mazzinghi,Alice Molli,Giulia Antonelli,Samuela Landini,Maria Elena Melica,Anna Julie Peired,Laura Maggi,Marta Donati,Gilda La Regina,Marco Allinovi,Fiammetta Ravaglia,Daniele Guasti,Daniele Bani,Luigi Cirillo,Francesca Becherucci,Francesco Guzzi,Alberto Magi,Francesco Annunziato,Laura Lasagni,Hans-Joachim Anders,Elena Lazzeri,Paola Romagnani

Cell reports 33:108525 PubMed33357427

2020

Single-Cell Transcriptomics Reveal Immune Mechanisms of the Onset and Progression of IgA Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zheng,Ping Lu,Yiyao Deng,Lu Wen,Yong Wang,Xin Ma,Zhongxin Wang,Lingling Wu,Quan Hong,Shuwei Duan,Zhong Yin,Bo Fu,Guangyan Cai,Xiangmei Chen,Fuchou Tang

Human gene therapy methods 29:251-258 PubMed30458119

2018

Transparenchymal Renal Pelvis Injection of Recombinant Adeno-Associated Virus Serotype 9 Vectors Is a Practical Approach for Gene Delivery in the Kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Xufeng Shen,Yuchen Xu,Zhengming Bai,Dongyue Ma,Qingsong Niu,Jialin Meng,Song Fan,Li Zhang,Zongyao Hao,Xiansheng Zhang,Chaozhao Liang

Cell death discovery 4:49 PubMed30345080

2018

Estrogen nuclear receptors affect cell migration by altering sublocalization of AQP2 in glioma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Wan,Juanjuan Jiang,Chuanming Zheng,Ning Wang,Xia Zhai,Xiangwei Fei,Ruijin Wu,Xiuxiu Jiang

Cell reports 24:3554-3567.e3 PubMed30257215

2018

Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Wang,Yidong Chen,Jun Yong,Yueli Cui,Rui Wang,Lu Wen,Jie Qiao,Fuchou Tang

Clinical and experimental pharmacology & physiology 43:1225-1233 PubMed27593225

2016

Brattleboro rats have impaired apical membrane water permeability regulation in the outer medullary collecting duct principal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Galina S Baturina,Liubov E Katkova,Sotirios G Zarogiannis,Evgeniy I Solenov

Journal of the American Society of Nephrology : JA 27:3220-3228 PubMed26984885

2016

Autoantibodies Targeting a Collecting Duct-Specific Water Channel in Tubulointerstitial Nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Nils Landegren,Mina Pourmousa Lindberg,Jakob Skov,Åsa Hallgren,Daniel Eriksson,Trine Lisberg Toft-Bertelsen,Nanna MacAulay,Eva Hagforsen,Anne Räisänen-Sokolowski,Heikki Saha,Thomas Nilsson,Gunnel Nordmark,Sophie Ohlsson,Jan Gustafsson,Eystein S Husebye,Erik Larsson,Mark S Anderson,Jaakko Perheentupa,Fredrik Rorsman,Robert A Fenton,Olle Kämpe

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