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Rabbit Recombinant Monoclonal Aquaporin 2 antibody. Suitable for mIHC, IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 12 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (AB199975), expandable thumbnail
  • Western blot - Anti-Aquaporin 2 antibody [EPR21080] (AB199975), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (AB199975), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (AB199975), expandable thumbnail
  • Immunoprecipitation - Anti-Aquaporin 2 antibody [EPR21080] (AB199975), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIPWBIHC-FrIHC-P
Human
Tested
Expected
Tested
Expected
Tested
Mouse
Expected
Tested
Tested
Tested
Tested
Rat
Expected
Expected
Expected
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/3000

Notes

-

Species

Rat

Dilution info

1/3000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:8140421, PubMed:7524315, PubMed:7510718, PubMed:15509592). Plays an essential role in renal water homeostasis (PubMed:8140421, PubMed:7524315, PubMed:15509592).

Alternative names

Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, AQP-CD, AQP2

Recommended products

Rabbit Recombinant Monoclonal Aquaporin 2 antibody. Suitable for mIHC, IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21080

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aquaporin 2 often referred to as AQP2 or aquaporin-2 channels is a water channel protein facilitating water transport across cell membranes. This protein belongs to the larger family of aquaporins which includes variants such as aquaporine often referred to interchangeably. Aquaporin 2 is a glycosylated integral membrane protein with an estimated mass of about 29 kDa. It expresses mainly in the kidney specifically in the collecting duct cells where it plays a pivotal role in water reabsorption.

Biological function summary

Aquaporin 2 serves a critical function in maintaining body water balance homeostasis. AQP2 responds to vasopressin a hormone that triggers its translocation to the apical membrane of collecting duct cells enabling water permeability. It is not part of a broader protein complex but operates independently within the cell membrane. This activity regulates urine concentration contributing to the maintenance of body fluids under variable hydration conditions.

Pathways

Aquaporin 2 contributes prominently to the vasopressin-regulated water reabsorption pathway and relates to the renal water reabsorption pathway. These pathways are important for interpreting how water homeostasis is managed by the body. Proteins such as the vasopressin receptor (also known as AVPR2) interact directly with AQP2 enabling its regulation and function. The interaction with cAMP signaling pathways facilitates the relocation of aquaporin 2 to its active site on the cell membrane ensuring efficient water reabsorption.

Associated diseases and disorders

Mutations or dysregulation in aquaporin 2 can cause conditions like nephrogenic diabetes insipidus characterized by an inability to concentrate urine leading to excessive urination and thirst. Another disorder associated with AQP2 is syndrome of inappropriate antidiuretic hormone secretion (SIADH). These conditions highlight the importance of aquaporin 2 in fluid homeostasis and they connect it to regulatory proteins such as vasopressin itself emphasizing the interconnected nature of cellular proteins in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of human kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Western blot - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds.

    We recommend customers optimize their lysis buffer and sample preparation for WB. This protein might aggregate during heating of samples.

    The molecular mass is consistent with literature (PMID 27405971; PMID 26109089).

    All lanes: Western blot - Anti-Aquaporin 2 antibody [EPR21080] (ab199975) at 1/500 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: Mouse kidney lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of mouse kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of rat kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Immunoprecipitation - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Aquaporin 2 was immunoprecipitated from 0.35 mg mouse kidney lysate with ab199975 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse kidney lysate 10 μg (input).
    Lane 2: ab199975 IP in mouse kidney lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199975 in mouse kidney lysate (-).

    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

    We recommend customers optimize their lysis buffer and sample preparation for IP. This protein might aggregate during heating of samples.

    The molecular mass is consistent with the literature (PMID 27405971; PMID 26109089).

    All lanes: Immunoprecipitation - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Predicted band size: 28 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on mouse kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Nuclear counterstain: DAPI (blue).
    Permeabilization: 0.2% Triton X-100

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on rat kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Nuclear counterstain: DAPI (blue).
    Permeabilization: 0.2% Triton X-100

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Multiplex immunohistochemistry - Anti-Aquaporin 2 antibody [EPR21080] (ab199975), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Fluorescence multiplex immunohistochemical analysis of the human kidney (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-Hexokinase 1 (Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker ab150423, green; Opal™690), anti-Angiotensin Converting Enzyme 1 (Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222, gray; Opal™520) and anti-Aquaporin 2 (ab199975, red; Opal™570) on human kidney. Panel B: anti-Aquaporin 2 stained on collecting tubules. Panel C: anti-Angiotensin Converting Enzyme 1 stained on proximal tubules. Panel D: anti-Hexokinase 1 stained on distal tubules and collecting tubules. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

    The section was incubated in three rounds of staining: in the order of Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker ab150423 at 1/250 dilution (4.224 μg/ml), Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222 at 1/4000 dilution (0.141 μg/ml) and ab199975 at 1/4000 dilution (0.152 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

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