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AB254072

Anti-Aquaporin 2 (phospho S269) antibody

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(2 Publications)

Rabbit Polyclonal Aquaporin 2 phospho S269 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications.

View Alternative Names

Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, AQP-CD, Aqp2

1 Images
Western blot - Anti-Aquaporin 2 (phospho S269) antibody (AB254072)
  • WB

Supplier Data

Western blot - Anti-Aquaporin 2 (phospho S269) antibody (AB254072)

All lanes:

Western blot - Anti-Aquaporin 2 (phospho S269) antibody (ab254072) at 1/1000 dilution

Lane 1:

Rat kidney (untreated) lysate

Lane 2:

Rat kidney (treated with vasopressin) lysate

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specific for endogenous levels of the ~29 kDa Aquaporin 2 protein phosphorylated at Ser269. Also recognizes the glycosylated form of Aquaporin 2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Storage buffer
pH: 7.5 Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 2 often referred to as AQP2 or aquaporin-2 channels is a water channel protein facilitating water transport across cell membranes. This protein belongs to the larger family of aquaporins which includes variants such as aquaporine often referred to interchangeably. Aquaporin 2 is a glycosylated integral membrane protein with an estimated mass of about 29 kDa. It expresses mainly in the kidney specifically in the collecting duct cells where it plays a pivotal role in water reabsorption.
Biological function summary

Aquaporin 2 serves a critical function in maintaining body water balance homeostasis. AQP2 responds to vasopressin a hormone that triggers its translocation to the apical membrane of collecting duct cells enabling water permeability. It is not part of a broader protein complex but operates independently within the cell membrane. This activity regulates urine concentration contributing to the maintenance of body fluids under variable hydration conditions.

Pathways

Aquaporin 2 contributes prominently to the vasopressin-regulated water reabsorption pathway and relates to the renal water reabsorption pathway. These pathways are important for interpreting how water homeostasis is managed by the body. Proteins such as the vasopressin receptor (also known as AVPR2) interact directly with AQP2 enabling its regulation and function. The interaction with cAMP signaling pathways facilitates the relocation of aquaporin 2 to its active site on the cell membrane ensuring efficient water reabsorption.

Mutations or dysregulation in aquaporin 2 can cause conditions like nephrogenic diabetes insipidus characterized by an inability to concentrate urine leading to excessive urination and thirst. Another disorder associated with AQP2 is syndrome of inappropriate antidiuretic hormone secretion (SIADH). These conditions highlight the importance of aquaporin 2 in fluid homeostasis and they connect it to regulatory proteins such as vasopressin itself emphasizing the interconnected nature of cellular proteins in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed : 7508187, PubMed : 8429910). Plays an essential role in renal water homeostasis (By similarity). Could also be permeable to glycerol (By similarity).
See full target information Aqp2 phospho S269
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:29650 PubMed39609591

2024

Goreisan promotes diuresis by regulating the abundance of aquaporin 2 phosphorylated at serine 269 through calcium-sensing receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Ogura,Naoki Fujitsuka,Miwa Nahata,Yohei Tokita

Frontiers in cardiovascular medicine 9:897244 PubMed35722114

2022

Evidence for a Prehypertensive Water Dysregulation Affecting the Development of Hypertension: Results of Very Early Treatment of Vasopressin V1 and V2 Antagonism in Spontaneously Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ignazio Verzicco,Stefano Tedeschi,Gallia Graiani,Alice Bongrani,Maria Luisa Carnevali,Simona Dancelli,Jessica Zappa,Silvia Mattei,Achiropita Bovino,Stefania Cavazzini,Rossana Rocco,Anna Calvi,Barbara Palladini,Riccardo Volpi,Valentina Cannone,Pietro Coghi,Alberico Borghetti,Aderville Cabassi
View all publications
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