AB125045

Anti-Aquaporin 3 antibody

Name: Anti-Aquaporin 3 antibody (ab125045) | Abcam
Brand: Abcam Limited
SKU: AB125045

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(6 Publications)

Rabbit Polyclonal Aquaporin 3 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Rat Aqp3 aa 250 to C-terminus.

View Alternative Names

Aquaporin-3, AQP-3, 31.4 kDa water channel protein, Aquaglyceroporin-3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody (AB125045)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody (AB125045)

ab125045, at 1/200 dilution, staining Aquaporin 3 in Rat kidney tissues by Immunofluorescence.

Unknown

Western blot - Anti-Aquaporin 3 antibody (AB125045)

All lanes:

Western blot - Anti-Aquaporin 3 antibody (ab125045) at 1/2000 dilution

All lanes:

Rat kidney lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Rat Aqp3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P47862

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/400", "ICCIF-species-notes": "<p></p>" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 3 (AQP3) also known as AQP-3 is a water and glycerol channel protein with a molecular weight of approximately 31 kDa. It facilitates the transport of water glycerol and other small solutes across cell membranes. AQP3 is expressed in a variety of tissues including the epidermis kidney respiratory tract and gastrointestinal tract. The protein's ability to transport glycerol is especially important in maintaining skin hydration and elasticity.

Biological function summary

Aquaporin channels such as AQP3 aid in the osmotic balance and are part of the cell membrane's permeability machinery. AQP3 forms functional homotetrameric structures that integrate into the plasma membrane. Each monomer of AQP3 contains a pore for the passage of water and other small molecules. This structural arrangement allows cells to regulate fluid movement and maintain homeostasis particularly in epithelial tissues.

Pathways

AQP3 engages in the regulation of transepithelial water movement and is involved in glycerol metabolism pathways. It interacts with proteins such as AQP1 and AQP4 supporting fluid transport across various organs. In epidermal cells AQP3 plays a role in the glycerol transport pathway important for skin barrier function and wound healing processes. Its role in these pathways highlights its contribution to cellular hydration and metabolic functions.

AQP3 has links to conditions like psoriasis and kidney dysfunction. Aberrant expression of AQP3 can contribute to skin disorders by disrupting hydration balance and affecting glycerol transport. In the kidney altered AQP3 expression impacts water reabsorption and can exacerbate renal pathologies. The interaction of AQP3 with other aquaporins like AQP1 suggests a collaborative role in disease progression through shared pathways involving fluid balance anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Aquaglyceroporins form homotetrameric transmembrane channels, with each monomer independently mediating glycerol and water transport across the plasma membrane along their osmotic gradient (PubMed : 7517548, PubMed : 7526388). Could also be permeable to urea (PubMed : 7517548, PubMed : 7526388). Also participates in cell permeability to H2O2 and H2O2-mediated signaling (By similarity). In skin, transports glycerol to the epidermis and stratum corneum, where it maintains hydration, elasticity, and supports lipid biosynthesis for barrier repair. In kidney, contributes to the reabsorption of water, helping the body maintain proper fluid balance (By similarity).

See full target information Aqp3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40710335

2025

Sigma1 Receptor Modulates Plasma Membrane and Mitochondrial Peroxiporins.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Pellavio,Giorgia Senise,Chiara Pia Vicenzo,Umberto Laforenza

International journal of molecular sciences 23: PubMed36142747

2022

Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Pellavio,Patrizia Sommi,Umberto Anselmi-Tamburini,Maria Paola DeMichelis,Stefania Coniglio,Umberto Laforenza

Cells 11: PubMed35741021

2022

Aquaporin-6 May Increase the Resistance to Oxidative Stress of Malignant Pleural Mesothelioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Pellavio,Simona Martinotti,Mauro Patrone,Elia Ranzato,Umberto Laforenza

TheScientificWorldJournal 2021:1393372 PubMed34887703

2021

Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Heba R Salem,Manar A Faried

International journal of molecular sciences 22: PubMed34575952

2021

Sigma-1 Receptor Agonists Acting on Aquaporin-Mediated HO Permeability: New Tools for Counteracting Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Pellavio,Giacomo Rossino,Giulia Gastaldi,Daniela Rossi,Pasquale Linciano,Simona Collina,Umberto Laforenza

Journal of the American Society of Nephrology : JA 28:2322-2336 PubMed28336721

2017

Tubule-Derived Wnts Are Required for Fibroblast Activation and Kidney Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhou,Haiyan Fu,Lu Zhang,Ke Zhang,Yali Min,Liangxiang Xiao,Lin Lin,Sheldon I Bastacky,Youhua Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Aquaporin 3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody (AB125045)

Western blot - Anti-Aquaporin 3 antibody (AB125045)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise