Name: Anti-Aquaporin 3 antibody
SKU: ab125219
Rating: 4 (5 reviews)

Anti-Aquaporin 3 antibody ab125219 is a rabbit polyclonal antibody that is used in Aquaporin 3 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- One antibody for all your Aquaporin 3 staining

Images

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody (AB125219), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Form: Liquid
Clonality: Polyclonal

Immunogen

Synthetic Peptide within Human AQP3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92482

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Expected
Rat	Tested	Expected	Tested	Expected
African green monkey	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 2-5 µg/mL	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 2-5 µg/mL	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 2-5 µg/mL	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 5 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 0.10000-0.50000 µg/mL	Notes -
Species Rat	Dilution info 0.10000-0.50000 µg/mL	Notes -
Species African green monkey	Dilution info 0.10000-0.50000 µg/mL	Notes -
Species Human	Dilution info 0.10000-0.50000 µg/mL	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1.00000-3.00000 µg/cells for 6.00000 µg/cells	Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information AQP3

Function

Aquaglyceroporins form homotetrameric transmembrane channels, with each monomer independently mediating glycerol and water transport across the plasma membrane along their osmotic gradient (PubMed:12239222, PubMed:30420639). Could also be permeable to urea (By similarity). Also participates in cell permeability to H2O2 and H2O2-mediated signaling (PubMed:20724658). In skin, transports glycerol to the epidermis and stratum corneum, where it maintains hydration, elasticity, and supports lipid biosynthesis for barrier repair (By similarity). In kidney, contributes to the reabsorption of water, helping the body maintain proper fluid balance (By similarity).

Alternative names

Aquaporin-3, AQP-3, Aquaglyceroporin-3, AQP3

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Form: Liquid
Clonality: Polyclonal
Immunogen: Synthetic Peptide within Human AQP3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92482
Purification technique: Affinity purification Immunogen
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aquaporin 3 (AQP3) also known as AQP-3 is a water and glycerol channel protein with a molecular weight of approximately 31 kDa. It facilitates the transport of water glycerol and other small solutes across cell membranes. AQP3 is expressed in a variety of tissues including the epidermis kidney respiratory tract and gastrointestinal tract. The protein's ability to transport glycerol is especially important in maintaining skin hydration and elasticity.

Biological function summary

Aquaporin channels such as AQP3 aid in the osmotic balance and are part of the cell membrane's permeability machinery. AQP3 forms functional homotetrameric structures that integrate into the plasma membrane. Each monomer of AQP3 contains a pore for the passage of water and other small molecules. This structural arrangement allows cells to regulate fluid movement and maintain homeostasis particularly in epithelial tissues.

Pathways

AQP3 engages in the regulation of transepithelial water movement and is involved in glycerol metabolism pathways. It interacts with proteins such as AQP1 and AQP4 supporting fluid transport across various organs. In epidermal cells AQP3 plays a role in the glycerol transport pathway important for skin barrier function and wound healing processes. Its role in these pathways highlights its contribution to cellular hydration and metabolic functions.

Associated diseases and disorders

AQP3 has links to conditions like psoriasis and kidney dysfunction. Aberrant expression of AQP3 can contribute to skin disorders by disrupting hydration balance and affecting glycerol transport. In the kidney altered AQP3 expression impacts water reabsorption and can exacerbate renal pathologies. The interaction of AQP3 with other aquaporins like AQP1 suggests a collaborative role in disease progression through shared pathways involving fluid balance anomalies.

Product promise

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12 product images

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody (ab125219)
Immunocytochemistry analysis of A431 (human epidermoid carcinoma cell line) cells labeling Aquaporin 3 with ab125219 at 2 μg/mL. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with ab125219 overnight at 4°C. DyLight^® 488 Conjugated Goat Anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI and visualized using a fluorescence microscope with filter sets appropriate for the label used.
Western blot - Anti-Aquaporin 3 antibody (ab125219)
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was then blocked with 5% Non-fat Milk / TBS for 1.5 hours at room temperature followed by incubation with ab125219. The membrane was then washed with TBS-0.1% Tween 3 times for 5 minutes each and probed with a Goat anti-rabbit IgG (HRP) secondary antibody.
All lanes: Western blot - Anti-Aquaporin 3 antibody (ab125219) at 0.5 µg/mL
Lane 1: Rat kidney tissue lysate at 50 µg
Lane 2: Mouse kidney tissue lysate at 50 µg
Lane 3: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 50 µg
Lane 4: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 50 µg
Secondary
All lanes: Goat Anti-rabbit IgG (HRP) for 1.5 hour at room temperature at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 32 kDa, 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody (ab125219)
Overlay histogram showing RT4 cells stained with ab125219 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with ab125219 (1µg/1x10⁶ cells) for 30 min at 20°C. DyLight^®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot - Anti-Aquaporin 3 antibody (ab125219)
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was then blocked with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 3 antigen affinity purified polyclonal antibody at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at room temperature.
All lanes: Western blot - Anti-Aquaporin 3 antibody (ab125219) at 0.5 µg/mL
Lane 1: HeLa whole cell lysates at 30 µg
Lane 2: Rat kidney tissue lysate at 30 µg
Lane 3: Mouse kidney tissue lysate at 30 µg
Secondary
All lanes: Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody (ab125219)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Aquaporin 3 with ab125219 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125219 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).