AB215853

Anti-Aquaporin 3 antibody [EPR19932]

Name: Anti-Aquaporin 3 antibody [EPR19932] (ab215853) | Abcam
Brand: Abcam Limited
SKU: AB215853
Price: 4680 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(3 Publications)

Rabbit Recombinant Monoclonal Aquaporin 3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Aquaporin-3, AQP-3, Aquaglyceroporin-3, AQP3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemical analysis of paraffin-embedded human skin squamous cell cancer tissue labeling Aquaporin 3 with ab215853 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on human skin squamous cell cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling Aquaporin 3 with ab215853 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on cervix cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 3 with ab215853 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on kidney collecting ducts is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Aquaporin 3 with ab215853 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on bladder cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Aquaporin 3 with ab215853 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on skin squamous cell is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) labelling Aquaporin 3 with ab215853 at 1/100 (5.03 μg/ml) dilution (Green). Confocal image showing mainly membranous staining in A431 cell line.

Negative control : U-87 MG

Nuclear DNA was labelled with DAPI (shown in blue). Secondary used was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5μg/ml) dilution (Magenta).

Negative control : U-87 MG

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Carrier free

Anti-Aquaporin 3 antibody [EPR19932] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Aquaporin 3 antibody [EPR19932]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Aquaporin 3 antibody [EPR19932]
660 APC

APC Anti-Aquaporin 3 antibody [EPR19932]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Aquaporin 3 antibody [EPR19932]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Aquaporin 3 antibody [EPR19932]
578 PE

PE Anti-Aquaporin 3 antibody [EPR19932]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Aquaporin 3 antibody [EPR19932]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19932

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 3 (AQP3) also known as AQP-3 is a water and glycerol channel protein with a molecular weight of approximately 31 kDa. It facilitates the transport of water glycerol and other small solutes across cell membranes. AQP3 is expressed in a variety of tissues including the epidermis kidney respiratory tract and gastrointestinal tract. The protein's ability to transport glycerol is especially important in maintaining skin hydration and elasticity.

Biological function summary

Aquaporin channels such as AQP3 aid in the osmotic balance and are part of the cell membrane's permeability machinery. AQP3 forms functional homotetrameric structures that integrate into the plasma membrane. Each monomer of AQP3 contains a pore for the passage of water and other small molecules. This structural arrangement allows cells to regulate fluid movement and maintain homeostasis particularly in epithelial tissues.

Pathways

AQP3 engages in the regulation of transepithelial water movement and is involved in glycerol metabolism pathways. It interacts with proteins such as AQP1 and AQP4 supporting fluid transport across various organs. In epidermal cells AQP3 plays a role in the glycerol transport pathway important for skin barrier function and wound healing processes. Its role in these pathways highlights its contribution to cellular hydration and metabolic functions.

AQP3 has links to conditions like psoriasis and kidney dysfunction. Aberrant expression of AQP3 can contribute to skin disorders by disrupting hydration balance and affecting glycerol transport. In the kidney altered AQP3 expression impacts water reabsorption and can exacerbate renal pathologies. The interaction of AQP3 with other aquaporins like AQP1 suggests a collaborative role in disease progression through shared pathways involving fluid balance anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Aquaglyceroporins form homotetrameric transmembrane channels, with each monomer independently mediating glycerol and water transport across the plasma membrane along their osmotic gradient (PubMed : 12239222, PubMed : 30420639). Could also be permeable to urea (By similarity). Also participates in cell permeability to H2O2 and H2O2-mediated signaling (PubMed : 20724658). In skin, transports glycerol to the epidermis and stratum corneum, where it maintains hydration, elasticity, and supports lipid biosynthesis for barrier repair (By similarity). In kidney, contributes to the reabsorption of water, helping the body maintain proper fluid balance (By similarity).

See full target information AQP3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 14:1351-1370 PubMed40386722

2025

was stabilized through N-methyladenosine methylation and exerted its anticancer effects in lung squamous cell carcinoma by inhibiting SIRT1 activity via CCAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Yan,Wingshing Wong,Jinsong Lei,Dachuan Liang,Jie Yang,Li Gong,Rossana Berardi,Shuqin Dai,Junye Wang

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:1160-1167 PubMed37946478

2023

Hetong decoction relieves loperamide-induced constipation in rats by regulating expression of aquaporins.

Applications

Unspecified application

Species

Unspecified reactive species

Huang Xi,Xia Youguang,Han Kai,Q I Weiwei,Linghu Qing,Zhang Qing,Xue Jingbo

Journal of the European Academy of Dermatology and Venereology : JEADV 33:e208-e209 PubMed30739344

2019

Epidermal aquaporin-3 downexpression in symmetrical acrokeratoderma.

Applications

Unspecified application

Species

Unspecified reactive species

M-L Rong,Z-W Zhang,Y Luo,G Shi,Y-M Fan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aquaporin 3 antibody [EPR19932]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 3 antibody [EPR19932] (AB215853)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise