Rabbit Recombinant Monoclonal Aquaporin 3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, mIHC and reacts with Human samples.
Images
![Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunoprecipitation-img432092.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970)")
![Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--multiplex-immunohistochemistry-img422539.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970)")
![Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--flow-cytometry-intracellular-img408904.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408903.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408902.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | ICC/IF | IP | IHC-Fr | mIHC | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Water channel required to promote glycerol permeability and water transport across cell membranes (PubMed:12239222, PubMed:30420639). Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).
Alternative names
Aquaporin-3, AQP-3, Aquaglyceroporin-3, AQP3
Recommended products
Rabbit Recombinant Monoclonal Aquaporin 3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, mIHC and reacts with Human samples.
Alternative names
Aquaporin-3, AQP-3, Aquaglyceroporin-3, AQP3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR28053-11
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Aquaporin 3 (AQP3) also known as AQP-3 is a water and glycerol channel protein with a molecular weight of approximately 31 kDa. It facilitates the transport of water glycerol and other small solutes across cell membranes. AQP3 is expressed in a variety of tissues including the epidermis kidney respiratory tract and gastrointestinal tract. The protein's ability to transport glycerol is especially important in maintaining skin hydration and elasticity.
- Biological function summary
Aquaporin channels such as AQP3 aid in the osmotic balance and are part of the cell membrane's permeability machinery. AQP3 forms functional homotetrameric structures that integrate into the plasma membrane. Each monomer of AQP3 contains a pore for the passage of water and other small molecules. This structural arrangement allows cells to regulate fluid movement and maintain homeostasis particularly in epithelial tissues.
- Pathways
AQP3 engages in the regulation of transepithelial water movement and is involved in glycerol metabolism pathways. It interacts with proteins such as AQP1 and AQP4 supporting fluid transport across various organs. In epidermal cells AQP3 plays a role in the glycerol transport pathway important for skin barrier function and wound healing processes. Its role in these pathways highlights its contribution to cellular hydration and metabolic functions.
- Associated diseases and disorders
AQP3 has links to conditions like psoriasis and kidney dysfunction. Aberrant expression of AQP3 can contribute to skin disorders by disrupting hydration balance and affecting glycerol transport. In the kidney altered AQP3 expression impacts water reabsorption and can exacerbate renal pathologies. The interaction of AQP3 with other aquaporins like AQP1 suggests a collaborative role in disease progression through shared pathways involving fluid balance anomalies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunoprecipitation-img432092.jpg "Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
Aquaporin 3 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2: Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 IP in A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 in A431 (human epidermoid carcinoma epithelial cell) whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] (Anti-Aquaporin 3 antibody [EPR28053-11] ab307969) at 1/30 dilution
All lanes: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Aquaporin 3 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2: Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 IP in A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 in A431 (human epidermoid carcinoma epithelial cell) whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
![Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--multiplex-immunohistochemistry-img422539.jpg "Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue labelling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 µg/mL) (B), Loricrin with Anti-Loricrin antibody [EPR7148(2)(B)] ab176322 at 1/3000 dilution (0.031 μg/ml) (C) and Filaggrin with Anti-Filaggrin antibody [EPR21892] ab221155 at 1/2000 dilution (0.523 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Aquaporin 3 (magenta; Opal™690), anti-Loricrin (green; Opal™520) and anti-Filaggrin (red; Opal™570) on human skin.
Panel B: anti-Aquaporin 3 stained on keratinocytes from the stratum basale to the stratum granulosum of the epidermis.
Panel C: anti-Loricrin stained on stratum corneum.
Panel D: anti-Filaggrin stained on the granular to stratum corneum transition.
The section was incubated in three rounds of staining: in the order of Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, Anti-Loricrin antibody [EPR7148(2)(B)] ab176322, and Anti-Filaggrin antibody [EPR21892] ab221155 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.![Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--flow-cytometry-intracellular-img408904.jpg "Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) (Left panel) compared to A431 (human epidermoid carcinoma epithelial cell) (Right panel) cells labelling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408903.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining on human cerebrum.
The section was incubated with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408902.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on human skin.
The section was incubated with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408901.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on human kidney.
The section was incubated with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--western-blot-img408840.jpg "Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
This data was developed using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time:180 secondsAll lanes: Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] (Anti-Aquaporin 3 antibody [EPR28053-11] ab307969) at 1/1000 dilution
All lanes: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds![Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--western-blot-img408841.jpg "Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)
This data was developed using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: brain(PMID: 10737773)
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time:180 seconds
Negative control: brain(PMID: 10737773)All lanes: Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] (Anti-Aquaporin 3 antibody [EPR28053-11] ab307969)
Lane 1: Human kidney tissue lysate at 20 mg/mL
Lane 2: Human brain tissue lysate at 20 mg/mL
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: brain(PMID: 10737773)
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
Negative control: brain(PMID: 10737773)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408901.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
![Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--western-blot-img408840.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
![Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail](https://content.abcam.com/products/images/aquaporin-3-antibody-epr28053-11-bsa-and-azide-free-ab307970--western-blot-img408841.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)")
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