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Rabbit Recombinant Monoclonal Aquaporin 3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, mIHC and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (AB307970), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)ICC/IFIPIHC-FrmIHC
Human
Tested
Tested
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Aquaglyceroporins form homotetrameric transmembrane channels, with each monomer independently mediating glycerol and water transport across the plasma membrane along their osmotic gradient (PubMed:12239222, PubMed:30420639). Could also be permeable to urea (By similarity). Also participates in cell permeability to H2O2 and H2O2-mediated signaling (PubMed:20724658). In skin, transports glycerol to the epidermis and stratum corneum, where it maintains hydration, elasticity, and supports lipid biosynthesis for barrier repair (By similarity). In kidney, contributes to the reabsorption of water, helping the body maintain proper fluid balance (By similarity).

Alternative names

Aquaporin-3, AQP-3, Aquaglyceroporin-3, AQP3

Recommended products

Rabbit Recombinant Monoclonal Aquaporin 3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, mIHC and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28053-11
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aquaporin 3 (AQP3) also known as AQP-3 is a water and glycerol channel protein with a molecular weight of approximately 31 kDa. It facilitates the transport of water glycerol and other small solutes across cell membranes. AQP3 is expressed in a variety of tissues including the epidermis kidney respiratory tract and gastrointestinal tract. The protein's ability to transport glycerol is especially important in maintaining skin hydration and elasticity.

Biological function summary

Aquaporin channels such as AQP3 aid in the osmotic balance and are part of the cell membrane's permeability machinery. AQP3 forms functional homotetrameric structures that integrate into the plasma membrane. Each monomer of AQP3 contains a pore for the passage of water and other small molecules. This structural arrangement allows cells to regulate fluid movement and maintain homeostasis particularly in epithelial tissues.

Pathways

AQP3 engages in the regulation of transepithelial water movement and is involved in glycerol metabolism pathways. It interacts with proteins such as AQP1 and AQP4 supporting fluid transport across various organs. In epidermal cells AQP3 plays a role in the glycerol transport pathway important for skin barrier function and wound healing processes. Its role in these pathways highlights its contribution to cellular hydration and metabolic functions.

Associated diseases and disorders

AQP3 has links to conditions like psoriasis and kidney dysfunction. Aberrant expression of AQP3 can contribute to skin disorders by disrupting hydration balance and affecting glycerol transport. In the kidney altered AQP3 expression impacts water reabsorption and can exacerbate renal pathologies. The interaction of AQP3 with other aquaporins like AQP1 suggests a collaborative role in disease progression through shared pathways involving fluid balance anomalies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.

    Aquaporin 3 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 IP in A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 in A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Aquaporin 3 antibody [EPR28053-11] (Anti-Aquaporin 3 antibody [EPR28053-11] ab307969) at 1/30 dilution

    All lanes: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 31 kDa

    Observed band size: 31 kDa

  • Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue labelling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 µg/mL) (B), Loricrin with Anti-Loricrin antibody [EPR7148(2)(B)] ab176322 at 1/3000 dilution (0.031 μg/ml) (C) and Filaggrin with Anti-Filaggrin antibody [EPR21892] ab221155 at 1/2000 dilution (0.523 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-Aquaporin 3 (magenta; Opal™690), anti-Loricrin (green; Opal™520) and anti-Filaggrin (red; Opal™570) on human skin.
    Panel B: anti-Aquaporin 3 stained on keratinocytes from the stratum basale to the stratum granulosum of the epidermis.
    Panel C: anti-Loricrin stained on stratum corneum.
    Panel D: anti-Filaggrin stained on the granular to stratum corneum transition.

    The section was incubated in three rounds of staining: in the order of Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, Anti-Loricrin antibody [EPR7148(2)(B)] ab176322, and Anti-Filaggrin antibody [EPR21892] ab221155 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) (Left panel) compared to A431 (human epidermoid carcinoma epithelial cell) (Right panel) cells labelling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Negative control: no staining on human cerebrum.

    The section was incubated with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining on human skin.

    The section was incubated with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    This data was produced using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same clone but in a different formulation.
    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining on human kidney.

    The section was incubated with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    This data was developed using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time:180 seconds

    All lanes: Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] (Anti-Aquaporin 3 antibody [EPR28053-11] ab307969) at 1/1000 dilution

    All lanes: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa

    Observed band size: 35 kDa

    Exposure time: 180s

  • Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970), expandable thumbnail

    Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] - BSA and Azide free (ab307970)

    This data was developed using Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: Negative control: brain(PMID: 10737773)

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time:180 seconds

    Negative control: brain(PMID: 10737773)

    All lanes: Western blot - Anti-Aquaporin 3 antibody [EPR28053-11] (Anti-Aquaporin 3 antibody [EPR28053-11] ab307969)

    Lane 1: Human kidney tissue lysate at 20 mg/mL

    Lane 2: Human brain tissue lysate at 20 mg/mL

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 31 kDa

    Observed band size: 35 kDa

    Exposure time: 180s

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