AB9512

Anti-Aquaporin 4 antibody [4/18]

Name: Anti-Aquaporin 4 antibody [4/18] (ab9512) | Abcam
Brand: Abcam Limited
SKU: AB9512
Price: 495 USD
Availability: InStock
Rating: 5 (7 reviews)

(7 Reviews)

(58 Publications)

Anti-Aquaporin 4 antibody [4/18] (ab9512) is a mouse monoclonal antibody detecting Aquaporin 4 in IHC-P, IHC-Fr, ELISA. Suitable for Human, Mouse, Rabbit, Rat, Zebrafish.

- Over 40 publications
- Trusted since 2002

View Alternative Names

Aquaporin-4, AQP-4, Mercurial-insensitive water channel, WCH4, MIWC, AQP4

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [4/18] (AB9512)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [4/18] (AB9512)

Immunohistochemical staining of aquaporin 4 in rat brain.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4/18

Isotype

IgG3

Light chain type

kappa

Carrier free

Reacts with

Rat, Zebrafish, Mouse, Rabbit, Human

Applications

IHC-FoFr, IHC-Fr, IHC-P, ELISA

applications

Immunogen

Synthetic Peptide within Rat Aqp4 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P47863

Epitope

Intracellular C-terminal AQP 4 epitope

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Aquaporin 4 antibody [4/18] (ab9512) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human, Mouse, Rabbit, Rat, Zebrafish samples.

Trusted by the scientific community
Anti-Aquaporin 4 [4/18] (ab9512) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Aquaporin 4 [4/18] (ab9512) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.097% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 4 also known as AQP4 functions as a water channel protein that facilitates the transport of water across cell membranes. It is composed of approximately 323 amino acids resulting in a molecular mass of around 34-38 kDa. This target expresses most in the central nervous system especially in the astrocytes of the brain and spinal cord. Other expression sites include skeletal muscle kidneys and lungs. AQP4 is often studied using various tools including anti-aquaporin 4 antibodies and specific reagents like AQP4 Ab and anti-AQP4.

Biological function summary

Water balance and neural activity regulation are significantly influenced by aquaporin 4. This protein forms a part of the orthogonal array of particles (OAPs) in the plasma membrane which enhances its water permeability function. Through its concentration at the blood-brain barrier AQP4 contributes to maintaining brain homeostasis by regulating osmotic gradients and extracellular space ion concentrations. Its expression and activity may affect critical processes like neuroexcitation and neuroprotection.

Pathways

Aquaporin 4 actively engages in critical water transport and blood-brain barrier integrity pathways. The passive yet selective water transport facilitated by AQP4 impacts pathways including the transcellular pathway that helps in maintaining the homeostatic and osmotic balance important for brain function. Furthermore AQP4 acts in relation to other proteins such as aquaporin 1 and connexin 43 indicating its role in gap junction communication within the brain.

Aquaporin 4 is intricately linked to conditions like neuromyelitis optica (NMO) and cerebral edema. In NMO autoantibodies such as anti-AQP4 target the protein causing demyelination and inflammation of the optic nerve and spinal cord. In cases of cerebral edema an imbalance in AQP4 expression can disrupt water homeostasis leading to exacerbated swelling. AQP4's interaction with proteins like myelin basic protein in these diseases underlines its significance in neurodegeneration and inflammatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a water-specific channel (PubMed : 19383790, PubMed : 7559426, PubMed : 8601457). Plays an important role in brain water homeostasis (PubMed : 37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).

See full target information AQP4

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Publications (58)

Recent publications for all applications. Explore the full list and refine your search

Non-coding RNA research 14:96-106 PubMed40529227

2025

MicroRNA-29a-5p attenuates hemorrhagic transformation and improves outcomes after mechanical reperfusion for acute ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Luo Li,Jin-Kun Zhuang,Zhong Liu,Zhong-Run Huang,Chun Xiang,Qian-Yu Chen,Ze-Xin Chen,Zhong-Song Shi

Journal of neuroinflammation 22:69 PubMed40055743

2025

Intravitreal delivery of NMO-IgG causes primary retinal damage in the absence of optic nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Biyue Chen,Huanfen Zhou,Mingming Sun,Wanqun Yang,Qianqian Li,Kaishu Yang,Honglu Song,Quangang Xu,Xintong Xu,Yuyu Li,Yanyan Yu,Shihui Wei,Tingjun Chen

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 282:2911-2920 PubMed39725698

2024

Morphological studies of labyrinthine tissue in patients affected with Meniere's disease and vestibular schwannoma following labyrinthectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Ankit Ajmera,Nikeith John,Adrienne Morey,Nigel Biggs,Sean Flanagan,Peter Earls,Daniel Brown,Payal Mukherjee

Aging and disease 16:3137-3153 PubMed39571157

2024

Differential Roles of Astrocytic CSF1 in Alzheimer's Disease and Cerebral Amyloid Angiopathy: Insights from Transcriptomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyuan Li,Yashuang Chen,Shiqi Luo,Yan Yang,Xinnan Liu,Sijie Li,Wei Ge,Cong Han

International journal of molecular sciences 25: PubMed38892076

2024

Anti-HMGB1 mAb Therapy Reduces Epidural Hematoma Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shangze Gao,Dengli Wang,Keyue Liu,Yasuko Tomono,Li Fu,Yuan Gao,Yohei Takahashi,Mariko Yata,Masahiro Nishibori

Aging and disease 15:2752-2769 PubMed38502590

2024

The identification of a Distinct Astrocyte Subtype that Diminishes in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Haichao Wei,Joseph Withrow,Jyotirmoy Rakshit,Faiz Ul Amin,Joshua Nahm,Francesca E Mowry,Zhengmei Mao,Meenakshi B Bhattacharjee,Jay-Jiguang Zhu,Yongjie Yang,Jia Qian Wu

Brain, behavior, & immunity - health 34:100684 PubMed37822873

2023

Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Wakid,Daniel Almeida,Zahia Aouabed,Reza Rahimian,Maria Antonietta Davoli,Volodymyr Yerko,Elena Leonova-Erko,Vincent Richard,René Zahedi,Christoph Borchers,Gustavo Turecki,Naguib Mechawar

eNeuro 10: PubMed37491366

2023

Single-Nucleus Profiling Identifies Accelerated Oligodendrocyte Precursor Cell Senescence in a Mouse Model of Down Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Rusu,Bharti Kukreja,Taiyi Wu,Sophie J Dan,Min Yi Feng,Brian T Kalish

The Journal of clinical investigation 133: PubMed37279069

2023

APOE-ε4 synergizes with sleep disruption to accelerate Aβ deposition and Aβ-associated tau seeding and spreading.

Applications

Unspecified application

Species

Unspecified reactive species

Chanung Wang,Aishwarya Nambiar,Michael R Strickland,Choonghee Lee,Samira Parhizkar,Alec C Moore,Erik S Musiek,Jason D Ulrich,David M Holtzman

Cancer research 83:1299-1314 PubMed36652557

2023

Active Remodeling of Capillary Endothelium via Cancer Cell-Derived MMP9 Promotes Metastatic Brain Colonization.

Applications

Unspecified application

Species

Unspecified reactive species

Matthia A Karreman,Alexander T Bauer,Gergely Solecki,Anna S Berghoff,Chanté D Mayer,Katharina Frey,Nils Hebach,Manuel J Feinauer,Nicole L Schieber,Cedric Tehranian,Luc Mercier,Mahak Singhal,Varun Venkataramani,Marc C Schubert,Daniel Hinze,Michael Hölzel,Iris Helfrich,Dirk Schadendorf,Stefan W Schneider,Dana Westphal,Hellmut G Augustin,Jacky G Goetz,Yannick Schwab,Wolfgang Wick,Frank Winkler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aquaporin 4 antibody [4/18]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [4/18] (AB9512)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (58)

Product promise