AB125049

Anti-Aquaporin 4 antibody

Name: Anti-Aquaporin 4 antibody (ab125049) | Abcam
Brand: Abcam Limited
SKU: AB125049
Price: 535 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal Aquaporin 4 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Rat Aqp4 aa 250-300.

View Alternative Names

Aquaporin-4, AQP-4, Mercurial-insensitive water channel, WCH4, MIWC, Aqp4

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 4 antibody (AB125049)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 4 antibody (AB125049)

ab125049, at 1/200 dilution, staining Aquaporin 4 in Rat kidney tissue by Immunofluorescence.

Unknown

Western blot - Anti-Aquaporin 4 antibody (AB125049)

All lanes:

Western blot - Anti-Aquaporin 4 antibody (ab125049) at 1/2000 dilution

All lanes:

Rat kidney lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Rat Aqp4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P47863

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/400", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 4 also known as AQP4 functions as a water channel protein that facilitates the transport of water across cell membranes. It is composed of approximately 323 amino acids resulting in a molecular mass of around 34-38 kDa. This target expresses most in the central nervous system especially in the astrocytes of the brain and spinal cord. Other expression sites include skeletal muscle kidneys and lungs. AQP4 is often studied using various tools including anti-aquaporin 4 antibodies and specific reagents like AQP4 Ab and anti-AQP4.

Biological function summary

Water balance and neural activity regulation are significantly influenced by aquaporin 4. This protein forms a part of the orthogonal array of particles (OAPs) in the plasma membrane which enhances its water permeability function. Through its concentration at the blood-brain barrier AQP4 contributes to maintaining brain homeostasis by regulating osmotic gradients and extracellular space ion concentrations. Its expression and activity may affect critical processes like neuroexcitation and neuroprotection.

Pathways

Aquaporin 4 actively engages in critical water transport and blood-brain barrier integrity pathways. The passive yet selective water transport facilitated by AQP4 impacts pathways including the transcellular pathway that helps in maintaining the homeostatic and osmotic balance important for brain function. Furthermore AQP4 acts in relation to other proteins such as aquaporin 1 and connexin 43 indicating its role in gap junction communication within the brain.

Aquaporin 4 is intricately linked to conditions like neuromyelitis optica (NMO) and cerebral edema. In NMO autoantibodies such as anti-AQP4 target the protein causing demyelination and inflammation of the optic nerve and spinal cord. In cases of cerebral edema an imbalance in AQP4 expression can disrupt water homeostasis leading to exacerbated swelling. AQP4's interaction with proteins like myelin basic protein in these diseases underlines its significance in neurodegeneration and inflammatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a water-specific channel (PubMed : 19800950, PubMed : 7509789, PubMed : 7528931). Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).

See full target information Aqp4

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40304567

2025

Cellular communication networks in fibrosis: Insights from the MASLD pig model.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyi Zhang,Jiakun Miao,Juan Du,Yu Yang,Boce Xia,Huanqi Peng,Shuang Xu,Jiangao Fan,Yanfang Wang,Martine Schroyen,Shulin Yang

International ophthalmology 44:437 PubMed39578251

2024

Carbonic anhydrase inhibitor alleviates retinal barrier toxicity in paclitaxel-induced retinopathy and macular edema by inhibiting CAXIV.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ting Ye,Ya-Li Niu,Zi-Yi Zhou,Yu Sun,Tian-Fang Chang,Yu-Tong Jing,Qian Bai,Zhao-Jie Chu

Glia 71:1921-1946 PubMed37029775

2023

GPR37L1 controls maturation and organization of cortical astrocytes during development.

Applications

Unspecified application

Species

Unspecified reactive species

TrangKimberly Thu Nguyen,Chad R Camp,Juleva K Doan,Stephen F Traynelis,Steven A Sloan,Randy A Hall

Nutrients 13: PubMed34836132

2021

Dose-Dependent Neuroprotective Effects of Bovine Lactoferrin Following Neonatal Hypoxia-Ischemia in the Immature Rat Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Sanches,Yohan van de Looij,Sadou Sow,Audrey Toulotte,Analina da Silva,Laura Modernell,Stéphane Sizonenko

Development (Cambridge, England) 148: PubMed34081130

2021

The histone demethylase PHF8 regulates astrocyte differentiation and function.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Iacobucci,Natalia Padilla,Martina Gabrielli,Claudia Navarro,Marta Lombardi,Marta Vicioso-Mantis,Claudia Verderio,Xavier de la Cruz,Marian A Martínez-Balbás

Frontiers in immunology 11:609476 PubMed33597947

2021

Relationship Between Pregnancy and Acute Disseminated Encephalomyelitis: A Single-Case Study.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwen Deng,Ke Qiu,Ranran Tu,Haiping Zheng,Wei Lu

ASN neuro 12:1759091420960550 PubMed32985231

2020

Alleviation of Cerebral Infarction of Rats With Middle Cerebral Artery Occlusion by Inhibition of Aquaporin 4 in the Supraoptic Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Cui,Shuwei Jia,Jiawei Yu,Dongyang Li,Tong Li,Yang Liu,Jinlong Chang,Xiaoran Wang,Xiaoyu Liu,Yu-Feng Wang

Annals of neurology 79:217-30 PubMed26506088

2016

Vascular Defects and Spinal Cord Hypoxia in Spinal Muscular Atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Eilidh Somers,Robert D Lees,Katie Hoban,James N Sleigh,Haiyan Zhou,Francesco Muntoni,Kevin Talbot,Thomas H Gillingwater,Simon H Parson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Aquaporin 4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 4 antibody (AB125049)

Western blot - Anti-Aquaporin 4 antibody (AB125049)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise