Anti-Aquaporin 4 antibody [EPR7040]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human cerebrum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human stomach. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

ab128906 at 1/100 dilution staining Aquaporin 4 in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat stomach. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat stomach (fresh) tissue labeling Aquaporin 4 with ab128906 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 μg/ml) dilution (Green). Positive staining on rat stomach. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 μg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse cerebrum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat cerebrum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse stomach. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse cerebellum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse kidney. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach (fresh) tissue labeling Aquaporin 4 with ab128906 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 μg/mL) dilution (Green). Positive staining on mouse stomach. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 μg/mL) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• WB

Lab

Western blot - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

'Unboil method' will optimize WB result.

The molecular weight is consistent with what has been described in the literature. (PMID : 22590566, 17897643).

All lanes:

Western blot - Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution

All lanes:

Human stomach lysate unboiled at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 30 kDa,28 kDa

false

Exposure time: 120s

• WB

Lab

Western blot - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. 'Unboil method' will  optimize WB result. Exposure : Lane1 & Lane2 : 20 seconds. Lane3 & Lane4 & Lane5 : 120 seconds.The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID : 18334967, 11742978, 17897643).

All lanes:

Western blot - Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution

Lane 1:

Mouse kidneyMouse kidney lysate unboiled lysate boiled at 20 µg

Lane 2:

Mouse brain lysate unboiled at 20 µg

Lane 3:

Rat stomach lysate unboiled at 20 µg

Lane 4:

Rat kidney lysate unboiled at 20 µg

Lane 5:

Human stomach lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 35 kDa

Observed band size: 100 kDa,28 kDa

false

• WB

Lab

Western blot - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. We recommend not to boil the samples after lysis to get desired WB results. The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID : 18334967, 11742978, 17897643).

All lanes:

Western blot - Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution

Lane 1:

Mouse brain lysate boiled at 15 µg

Lane 2:

Mouse brain lysate unboiled at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 35 kDa

Observed band size: 100 kDa,28 kDa

false

Exposure time: 60s

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Aquaporin 4 antibody [EPR7040] (AB128906)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.