Rabbit Recombinant Monoclonal Aquaporin 4 antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | WB | IHC-Fr | |
---|---|---|---|
Human | Tested | Tested | Expected |
Mouse | Tested | Tested | Tested |
Rat | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Forms a water-specific channel (PubMed:19383790, PubMed:7559426, PubMed:8601457). Plays an important role in brain water homeostasis (PubMed:37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
Aquaporin-4, AQP-4, Mercurial-insensitive water channel, WCH4, MIWC, AQP4
Rabbit Recombinant Monoclonal Aquaporin 4 antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR7040
Affinity purification Protein A
2.6 x 10-12 M
Blue Ice
+4°C
+4°C
Do Not Freeze
ab248213 is the carrier-free version of Anti-Aquaporin 4 antibody [EPR7040] ab128906.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Aquaporin 4 also known as AQP4 functions as a water channel protein that facilitates the transport of water across cell membranes. It is composed of approximately 323 amino acids resulting in a molecular mass of around 34-38 kDa. This target expresses most in the central nervous system especially in the astrocytes of the brain and spinal cord. Other expression sites include skeletal muscle kidneys and lungs. AQP4 is often studied using various tools including anti-aquaporin 4 antibodies and specific reagents like AQP4 Ab and anti-AQP4.
Water balance and neural activity regulation are significantly influenced by aquaporin 4. This protein forms a part of the orthogonal array of particles (OAPs) in the plasma membrane which enhances its water permeability function. Through its concentration at the blood-brain barrier AQP4 contributes to maintaining brain homeostasis by regulating osmotic gradients and extracellular space ion concentrations. Its expression and activity may affect critical processes like neuroexcitation and neuroprotection.
Aquaporin 4 actively engages in critical water transport and blood-brain barrier integrity pathways. The passive yet selective water transport facilitated by AQP4 impacts pathways including the transcellular pathway that helps in maintaining the homeostatic and osmotic balance important for brain function. Furthermore AQP4 acts in relation to other proteins such as aquaporin 1 and connexin 43 indicating its role in gap junction communication within the brain.
Aquaporin 4 is intricately linked to conditions like neuromyelitis optica (NMO) and cerebral edema. In NMO autoantibodies such as anti-AQP4 target the protein causing demyelination and inflammation of the optic nerve and spinal cord. In cases of cerebral edema an imbalance in AQP4 expression can disrupt water homeostasis leading to exacerbated swelling. AQP4's interaction with proteins like myelin basic protein in these diseases underlines its significance in neurodegeneration and inflammatory processes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach (fresh) tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 μg/ml) dilution (Green). Positive staining on mouse stomach. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 μg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat stomach (fresh) tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 μg/ml) dilution (Green). Positive staining on rat stomach. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 μg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat stomach.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cerebrum.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse stomach.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebrum.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebellum.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human cerebrum.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Aquaporin 4 with Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human stomach.The section was incubated with Anti-Aquaporin 4 antibody [EPR7040] ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formulation.Anti-Aquaporin 4 antibody [EPR7040] ab128906 at 1/100 dilution, staining Aquaporin 4 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
'Unboil method' will optimize WB result.
The molecular weight is consistent with what has been described in the literature. (PMID: 22590566, 17897643).
All lanes: Western blot - Anti-Aquaporin 4 antibody [EPR7040] (Anti-Aquaporin 4 antibody [EPR7040] ab128906) at 1/1000 dilution
All lanes: Human stomach lysate unboiled at 15 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 30 kDa, 28 kDa
Exposure time: 120s
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: 5% NFDM/TBST. 'Unboil method' will optimize WB result. Exposure: Lane1 & Lane2: 20 seconds. Lane3 & Lane4 & Lane5: 120 seconds.The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID:18334967, 11742978, 17897643).
All lanes: Western blot - Anti-Aquaporin 4 antibody [EPR7040] (Anti-Aquaporin 4 antibody [EPR7040] ab128906) at 1/1000 dilution
Lane 1: Mouse kidneyMouse kidney lysate unboiled lysate boiled at 20 µg
Lane 2: Mouse brain lysate unboiled at 20 µg
Lane 3: Rat stomach lysate unboiled at 20 µg
Lane 4: Rat kidney lysate unboiled at 20 µg
Lane 5: Human stomach lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 100 kDa, 28 kDa
This data was developed using Anti-Aquaporin 4 antibody [EPR7040] ab128906, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: 5% NFDM/TBST. We recommend not to boil the samples after lysis to get desired WB results. The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID:18334967, 11742978, 17897643).
All lanes: Western blot - Anti-Aquaporin 4 antibody [EPR7040] (Anti-Aquaporin 4 antibody [EPR7040] ab128906) at 1/1000 dilution
Lane 1: Mouse brain lysate boiled at 15 µg
Lane 2: Mouse brain lysate unboiled at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 100 kDa, 28 kDa
Exposure time: 60s
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