Anti-Aquaporin 5 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody (AB78486)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining Aquaporin 5 with ab78486 (1/2000) in rat lung. An anti-Rabbit secondary antibody and HRP-DAB was used. Tissues were processed using heat-induced epitope retrieval.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody (AB78486)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining Aquaporin 5 with ab78486 (1/500) in mouse trachea tissue. An anti-Rabbit secondary antibody and HRP-DAB was used. Tissues were processed using heat-induced epitope retrieval.

• WB

Supplier Data

Western blot - Anti-Aquaporin 5 antibody (AB78486)

All lanes:

Western blot - Anti-Aquaporin 5 antibody (ab78486) at 0.1 µg/mL

All lanes:

Rat salivary gland tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG secondary antibody conjugated to HRP

Predicted band size: 28 kDa

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• IHC

CiteAb

Immunohistochemistry - Anti-Aquaporin 5 antibody (AB78486)

Immunohistochemistry-immunofluorescence using Anti-Aquaporin 5 antibody, ab78486. Publication image from Adler, K. B. et al., 2020, Nat Commun, 32111836. Legend direct from paper.

Lung repair and fibrosis in mice after silica-injury.a The experimental study schematic of the silica-induced fibrosis study in A/J mice; n = 10 biological independent animals per group. b Representative TUNEL staining of apoptotic cells for each treatment group and quantification of percent of Tunel positive cells; Scale bar = 50 µm; each dot represents data from one animal; n = 5 biological independent animals. c Representative H&E staining. Scale bar = 100 µm. d Quantification of fibrosis by Ashcroft score; each dot represents data from one animal; n = 8 biological independent animals. Ashcroft score was performed by averaging the score from two blinded and one non-blinded scorer. e Gomori’s trichrome staining : muscle fibers (red), collagen (blue), nuclei (black-purple) and erythrocytes (red). Scale bar = 100 µm. f Representative picrosirius red staining; Collagen types I and III (red). Scale bar = 100 µm. g Quantification of pulmonary hydroxyproline levels; each dot represents data from one animal; n = 4 biological independent animals. h Representative immunostaining of lung sections for aquaporin 5 (AQP5), pro-surfactant protein C (Pro-SPC) and von Willebrand Factor (vWF) Scale bar = 50 µm. i–k Quantification of percent pixel intensity of AQP5+ (i), ProSPC+ (j), and vWF+ (k). Each dot represents data from one animal; n = 4 biological independent animals. Throughout, data are mean ± s.d. P-value as indicated by non-parametric one-way ANOVA; NS, not significant.

• IHC

CiteAb

Immunohistochemistry - Anti-Aquaporin 5 antibody (AB78486)

Immunohistochemistry-immunofluorescence using Anti-Aquaporin 5 antibody, ab78486. Publication image from Adler, K. B. et al., 2020, Nat Commun, 32111836. Legend direct from paper.

Therapeutic potential of exosome inhalation treatment in rats with pulmonary fibrosis.a Size analysis of fresh, frozen and lyophilized exosome particles by NanoSight. b Representative transmission electron micrograph (TEM) of LSC-Exo. Left scale bar = 0.2 µm. Right scale bar = 50 nm. c Immunoblot analysis of CD63, CD81, TSG101, and CD9 protein in LSC-Exo and MSC-Exo. d The experimental study schematic of the exosome study in SD rats; n = 12 biological independent animals per group. e Representative H&E staining Top : Scale bar = 100 µm Bottom : Scale bar = 50 µm. f Representative Gomori’s trichrome staining Scale bar = 100 µm (bottom); muscle fibers (red), collagen (blue), nuclei (black-purple) and erythrocytes (red). g Representative picrosirius red staining; Collagen types I and III (red); Scale bar = 50 µm. h Quantification of fibrosis by Ashcroft score; Each dot represents data from one animal; n = 12 biological independent animals. Ashcroft score was performed by averaging the score from one blinded and one non-blinded scorer. i Quantification of pulmonary hydroxyproline levels; *P ≤ 0.05; each dot represents data from one animal; n = 4 biological independent animals. j Immunoblot analysis of matrix metalloproteinase 2 (MMP-2), alpha smooth muscle actin (αSMA), SMAD3 and GAPDH loading control. k Representative immunostaining of lung sections for aquaporin 5 (AQP5), alpha-smooth muscle actin (αSMA), and von Willebrand Factor (vWF). Scale bar = 75 µm. l–n Quantification of pixel intensity of AQP5 (l),α-SMA (m), and vWF (n). Each dot represents data from one animal; n = 4 biological independent animals. Throughout, data are mean ± s.d. P-value as indicated by non-parametric one-way ANOVA.

• WB

CiteAb

Western blot - Anti-Aquaporin 5 antibody (AB78486)

Western Blotting using Anti-Aquaporin 5 antibody, ab78486. Publication image from Adler, K. B. et al., 2020, Nat Commun, 32111836. Legend direct from paper.

LSC-Sec inhalation treatment promotes alveolar repair.a Representative immunostaining of lung sections for von Willebrand Factor (vWF), pro-surfactant protein C (Pro-SPC) and aquaporin 5 (AQP5). Scale bar = 100 µm. b–d Quantification of percent pixel intensity of vWF+ (b), percent ProSPC+ nuclei (c), and percent pixel intensity of AQP5+ (d); each dot represents data from one animal; n = 12 biological independent animals. e–f Immunoblot analysis of aquaporin 5 (AQP5), pro-surfactant protein C (Pro-SPC), von Willebrand Factor (vWF), alpha smooth muscle actin (αSMA), SMAD3, matrix metalloproteinase 2 (MMP-2), and beta-actin loading control (B-actin) from lung protein lysate (e) with corresponding quantification of protein levels as fold of sham control (f); each dot represents data from one animal; n = 3 biological independent animals. g–h Representative cytokine array with quantification of relative intensity (g) and corresponding quantification of relative intensity (h). Throughout, data are mean ± s.d. P-value as indicated by non-parametric one-way ANOVA.

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